A silicon nitride ion sensitive field effect transistor‐based immunosensor for determination of urinary albumin

Saengdee, Pawasuth; Thanapitak, Surachoke; Ongwattanakul, Songpol; Srisuwan, Awirut; Pankiew, Apirak; Thornyanadacha, Nutthaphat; Chaisriratanakul, Woraphan; Jeamsaksiri, Wutthinan; Promptmas, Chamras

doi:10.1002/elsa.202100078

Cited by 5 publications

(2 citation statements)

References 29 publications

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“…As shown in Table 2 , the coefficient of variation (%CV) was calculated to be 10.69% for anti-HLA and 8.92% for anti-MICA, suggesting that acceptable reproducibility was obtained from the proposed biosensor. The reproducibility of this study is in accordance with the previous report, which utilized the Si 3 N 4 -ISFET immunosensor to detect the urinary albumin that reported the %CV of the inter-assay to detect 100 µg/mL of the human serum albumin: 9.67% [ 25 ].…”

Section: Resultssupporting

confidence: 90%

Biosensors Based on Ion-Sensitive Field-Effect Transistors for HLA and MICA Antibody Detection in Kidney Transplantation

et al. 2022

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This work demonstrates the ability of the Ion-Sensitive Field-Effect Transistor (ISFET)-based immunosensor to detect antibodies against the human leukocyte antigen (HLA) and the major histocompatibility complex class-I-related chain A (MICA). The sensing membrane of the ISFET devices was modified and functionalized using an APTES-GA strategy. Surface properties, including wettability, surface thickness, and surface topology, were assessed in each module of the modification process. The optimal concentrations of HLA and MICA proteins for the immobilization were 10 and 50 μg/mL. The dose-response curve showed a detection range of 1.98–40 µg/mL for anti-HLA and 5.17–40 µg/mL for anti-MICA. The analytical precision (%CV) was found to be 10.69% and 8.92% for anti-HLA and -MICA, respectively. Moreover, the electrical signal obtained from the irrelevant antibody was considerably different from that of the specific antibodies, indicating the specific binding of the relevant antibodies without noise interference. The sensitivity and specificity in the experimental setting were established for both antibodies (anti-HLA: sensitivity = 80.00%, specificity = 86.36%; anti-MICA: sensitivity = 86.67%, specificity = 88.89%). Our data reveal the potential of applying the ISFET-based immunosensor to the detection of relevant anti-HLA and -MICA antibodies, especially in the field of kidney transplantation.

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Section: Resultssupporting

confidence: 90%

Biosensors Based on Ion-Sensitive Field-Effect Transistors for HLA and MICA Antibody Detection in Kidney Transplantation

et al. 2022

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“…Nevertheless, there are only a small number of immunosensors reported in these arenas. A silicon-based ISFET has been reported for the detection of urinary albumin [72]. Herein, Saengdee has developed a low-cost immunosensor based on the binding event between the recombinant Ag85B antigen and anti-Ag85B antibody on the ISFET surface by monitoring the gate potential change at a constant drain current.…”

Section: Functional Model Of Bonding Between the Protein And The Unde...mentioning

confidence: 99%

Modelling Prospects of Bio-Electrochemical Immunosensing Platforms

Gandhi

2024

Electrochem

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Electrochemistry is a hotspot in today’s research arena. Many different domains have been extended for their role towards the Internet of Things, digital health, personalized nutrition, and/or wellness using electrochemistry. These advances have led to a substantial increase in the power and popularity of electroanalysis and its expansion into new phases and environments. The recent COVID-19 pandemic, which turned our lives upside down, has helped us to understand the need for miniaturized electrochemical diagnostic platforms. It also accelerated the role of mobile and wearable, implantable sensors as telehealth systems. The major principle behind these platforms is the role of electrochemical immunoassays, which help in overshadowing the classical gold standard methods (reverse transcriptase polymerase chain reaction) in terms of accuracy, time, manpower, and, most importantly, economics. Many research groups have endeavoured to use electrochemical and bio-electrochemical tools to overcome the limitations of classical assays (in terms of accuracy, accessibility, portability, and response time). This review mainly focuses on the electrochemical technologies used for immunosensing platforms, their fabrication requirements, mechanistic objectives, electrochemical techniques involved, and their subsequent output signal amplifications using a tagged and non-tagged system. The combination of various techniques (optical spectroscopy, Raman scattering, column chromatography, HPLC, and X-ray diffraction) has enabled the construction of high-performance electrodes. Later in the review, these combinations and their utilization will be explained in terms of their mechanistic platform along with chemical bonding and their role in signal output in the later part of article. Furthermore, the market study in terms of real prototypes will be elaborately discussed.

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2D Materials in Advanced Electronic Biosensors for Point‐of‐Care Devices

Nisar,

Dastgeer,

Shazad

et al. 2024

Advanced Science

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Since two‐dimensionalal (2D) materials have distinct chemical and physical properties, they are widely used in various sectors of modern technologies. In the domain of diagnostic biodevices, particularly for point‐of‐care (PoC) biomedical diagnostics, 2D‐based field‐effect transistor biosensors (bio‐FETs) demonstrate substantial potential. Here, in this review article, the operational mechanisms and detection capabilities of biosensing devices utilizing graphene, transition metal dichalcogenides (TMDCs), black phosphorus, and other 2D materials are addressed in detail. The incorporation of these materials into FET‐based biosensors offers significant advantages, including low detection limits (LOD), real‐time monitoring, label‐free diagnosis, and exceptional selectivity. The review also highlights the diverse applications of these biosensors, ranging from conventional to wearable devices, underscoring the versatility of 2D material‐based FET devices. Additionally, the review provides a comprehensive assessment of the limitations and challenges faced by these devices, along with insights into future prospects and advancements. Notably, a detailed comparison of FET‐based biosensors is tabulated along with various other biosensing platforms and their working mechanisms. Ultimately, this review aims to stimulate further research and innovation in this field while educating the scientific community about the latest advancements in 2D materials‐based biosensors.

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A silicon nitride ion sensitive field effect transistor‐based immunosensor for determination of urinary albumin

Cited by 5 publications

References 29 publications

Biosensors Based on Ion-Sensitive Field-Effect Transistors for HLA and MICA Antibody Detection in Kidney Transplantation

Biosensors Based on Ion-Sensitive Field-Effect Transistors for HLA and MICA Antibody Detection in Kidney Transplantation

Modelling Prospects of Bio-Electrochemical Immunosensing Platforms

2D Materials in Advanced Electronic Biosensors for Point‐of‐Care Devices

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