A simple and efficient Agrobacterium tumefaciens mediated transgenic system for tetraploid potato cultivar Desiree

Abstract

As the fourth staple food crop in the world, potatoes can provide enough energy, protein, and nutrients necessary for humans. However, the population growth and negative effects of climate change call for improved potato yields and resilience. Genetic engineering is a convenient way to improve potato varieties and create new germplasm resources. Agrobacterium tumefaciens mediated transformation is a stable and widely used method for genetic improvement. In this study, a simple and efficient transgenic system was built by testing different potato materials, explants type, selection medium, selection agent, A. tumefaciens EHA105 strain carried different vectors. The results shown this simple system produced positive transformed seedlings in about 40 days. The lower ploidy change ratio for tetraploid variety Desiree compared to diploid recipient. Both SEG and SE could function as a single regeneration medium. Leaf discs explants is eligible. The concentration of 20μg/L is suitable for chlorsulfuron selection. The positive transgenic efficiency of tetraploid recipient Desiree is up to 68.79%, This simple and fast transgenic system provides a powerful tool for supporting basic research on potato functional genes and creating new potato materials for genetic engineering breeding.