A simple and inexpensive automated technique for measurement of serum nitrite/nitrate

Tatsch, Etiane; Bochi, Guilherme Vargas; Pereira, Renata da Silva; Kober, Helena; Agertt, Vanessa Albertina; Campos, Marli Matiko Anraku de; Gomes, Patrícia; Duarte, Marta Maria Medeiros Frescura; Moresco, Rafael Noal

doi:10.1016/j.clinbiochem.2010.12.011

Cited by 262 publications

(116 citation statements)

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“…Inclusion criteria were patients with overweight or obese (BMI of 25 or more), T2DM aged 40-85 years old and with stable CHD condition. According to the criteria of American Diabetes Association [19], patients who have one out of three of the following criteria were diagnosed with T2DM: fasting plasma glucose (FPG) ≥ 126 mg/dL, blood glucose 2-hpp ≥ 200 mg/dL and had HbA1C ≥ 6.5 %. In addition, patients who had one or more of the following criteria were considered as stable CHD condition: record of myocardial infarction, document of at least 50 % stenosis in one or more coronary vessels upon cardiac catheterization evaluated by the angiography, document of the exercise-induced ischemia by treadmill electrocardiogram or nuclear perfusion stress imaging and a history of coronary revascularization [17].…”

Section: Participantsmentioning

confidence: 99%

The effects of coenzyme Q10 administration on glucose homeostasis parameters, lipid profiles, biomarkers of inflammation and oxidative stress in patients with metabolic syndrome

et al. 2015

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Section: Participantsmentioning

confidence: 99%

The effects of coenzyme Q10 administration on glucose homeostasis parameters, lipid profiles, biomarkers of inflammation and oxidative stress in patients with metabolic syndrome

et al. 2015

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“…As the macrophage activation involves increase in the nitric oxide (NO) levels, NO quantification was performed after 24 h PBMCs exposure to haloperidol and risperidone by applying the modified Griess method previously described by Tatsch et al (2011). The assay quantifies nitrite/nitrate production using the Cobas Mira automated analyzer.…”

Section: Nitric Oxide Quantificationmentioning

confidence: 99%

Haloperidol and Risperidone at high concentrations activate an in vitro inflammatory response of RAW 264.7 macrophage cells by induction of apoptosis and modification of cytokine levels

et al. 2015

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Antipsychotic drugs, such as haloperidol and risperidone, are used in long-term treatment of psychiatric patients and thus increase the risk of obesity and other metabolic dysfunctions. Available evidence suggests that these drugs have pro-inflammatory effect, which contributes to the establishment of endocrine disturbances. However, results yielded by extant studies are inconsistent. Therefore, in this work, we tested the in vitro effects of different high concentrations of haloperidol and risperidone on the activation of isolated macrophages (RAW 264.7 cell line). The results indicated that macrophages were activated by both drugs. In addition, the activation involved an increase in nitric oxide levels and apoptosis events by modulation of caspases 8 and 3 levels and a decrease of the Bcl-2/BAX gene expression ratio. Cells treated with haloperidol and risperidone also presented higher concentrations of inflammatory cytokines (IL-1β, IL-6, TNFα) and low levels of IL-6 anti-inflammatory cytokine in a dose-dependent manner. Despite the limitation of cell line studies based solely on macrophages cells, we suggest that antipsychotic drugs could potentially exacerbate inflammatory processes in peripheral tissues (blood and fat). The continued activation of macrophages could contribute to the development of obesity and other endocrine disturbances caused by the use of antipsychotic drugs.

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“…Serum high-sensitivity C-reactive protein (hs-CRP) concentrations were evaluated by commercial ELISA kit (LDN, Nordhorn, Germany) with intra- and inter-assay CVs of 2.9 and 4.9%, respectively. The plasma nitric oxide (NO) concentrations were assessed using the Griess method [23]. Plasma total antioxidant capacity (TAC) concentrations were determined by the method of ferric-reducing antioxidant power developed by Benzie and Strain [24], total glutathione (GSH) levels were determined using the method of Beutler and Gelbart [25] and malondialdehyde (MDA) concentrations were determined by the thiobarbituric acid reactive substances spectrophotometric test [26].…”

Section: Methodsmentioning

confidence: 99%

The Effects of Folate Supplementation on Carotid Intima-Media Thickness and Metabolic Status in Patients with Metabolic Syndrome

Talari¹,

Rafiee²,

Farrokhian³

et al. 2016

Ann Nutr Metab

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Background: This study was carried out to evaluate the effects of folate supplementation on carotid intima-media thickness (CIMT) and metabolic status among patients with metabolic syndrome (MetS). Methods: This randomized, double-blind, placebo-controlled trial was conducted among 60 patients with type 2 diabetes mellitus and coronary heart disease. They were all overweight in the age range 40-85 years. Participants were randomly divided into 2 groups: group A (n = 30) received 5 mg folate supplements and group B (n = 30) received placebo for 12 weeks. Results: Folate supplementation resulted in a significant reduction in maximum levels of left CIMT (-0.05 ± 0.13 vs. +0.02 ± 0.11 mm, p = 0.01) compared with the placebo. Changes in fasting plasma glucose (-2.2 ± 37.5 vs. +30.2 ± 65.8 mg/dl, p = 0.02), serum insulin concentration (-2.0 ± 10.7 vs. +3.0 ± 7.6 µIU/ml, p = 0.04) and homeostasis of assessment-estimated insulin resistance (-0.6 ± 2.3 vs. +0.9 ± 2.3, p = 0.01) in supplemented patients were significantly different from those of patients in the placebo group. Changes in serum triglycerides (p = 0.04), high-density lipoprotein-cholesterol (p = 0.001), high sensitivity C-reactive protein (p = 0.01) and plasma nitric oxide concentrations (p < 0.001) were significantly different between the supplemented patients and placebo group. Conclusions: Overall, 5 mg/day folate supplementation for 12 weeks among patients with MetS had beneficial effects on CIMT and the metabolic status.

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A simple and inexpensive automated technique for measurement of serum nitrite/nitrate

Cited by 262 publications

References 9 publications

The effects of coenzyme Q10 administration on glucose homeostasis parameters, lipid profiles, biomarkers of inflammation and oxidative stress in patients with metabolic syndrome

The effects of coenzyme Q10 administration on glucose homeostasis parameters, lipid profiles, biomarkers of inflammation and oxidative stress in patients with metabolic syndrome

Haloperidol and Risperidone at high concentrations activate an in vitro inflammatory response of RAW 264.7 macrophage cells by induction of apoptosis and modification of cytokine levels

The Effects of Folate Supplementation on Carotid Intima-Media Thickness and Metabolic Status in Patients with Metabolic Syndrome

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