A Simple and Rapid Laboratory Method for Determination of Haemostasis Potential in Plasma

He, Shuting; Antovič, Aleksandra; Blombäck, Margareta

doi:10.1016/s0049-3848(01)00332-2

Cited by 129 publications

(108 citation statements)

References 10 publications

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“…A more sensitive assay for fibrin generation was used on the basis of a method described previously 36,37 and modified by us. 38 Fibrin generation curves were created by recalcination of rats' plasma samples directly in microplate wells with CaCl 2 (36 mmol/L) dissolved in Tris buffer (66-mmol/L Tris; 130-mmol/L NaCl; pH=7.4) in 37°C.…”

Section: Coagulation Parameters and Fibrin Generationmentioning

confidence: 99%

Antithrombotic Properties of Water-Soluble Carbon Monoxide-Releasing Molecules

Kramkowski

Leszczyńska

Mogielnicki

et al. 2012

ATVB

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Objective-We compared the antithrombotic effects in vivo of 2 chemically different carbon monoxide-releasing molecules (CORM-A1 and CORM-3) on arterial and venous thrombus formation and on hemostatic parameters such as platelet activation, coagulation, and fibrinolysis. The hypotensive response to CORMs and their effects on whole blood gas analysis and blood cell count were also examined. Methods and Results-CORM-A1 (10-30 µmol/kg, i.v.), in a dose-dependent fashion, significantly decreased weight of electrically induced thrombus in rats, whereas CORM-3 inhibited thrombosis only at the highest dose used (30 µmol/kg). CORM-A1 showed a direct and stronger inhibition of platelet aggregation than CORM-3 in healthy rats, both in vitro and in vivo. The antiaggregatory effect of CORM-A1, but not CORM-3, correlated positively with weight of the thrombus. Concentration of active plasminogen activator inhibitor-1 in plasma also decreased in response to CORM-A1, but not to CORM-3. Neither CORM-A1 nor CORM-3 had an effect on plasma concentration of active tissue plasminogen activator. CORM-3, but not CORM-A1, decreased the concentration of fibrinogen, fibrin generation, and prolonged prothrombin time. Similarly, laser-induced venous thrombosis observed intravitally via confocal system in green fluorescent protein mice was significantly decreased by CORMs. Although both CORM-A1 and CORM-3 (30 µmol/kg) decreased platelets accumulation in thrombus, only CORM-A1 (3-30 µmol/kg) inhibited platelet activation to phosphatidylserine on their surface. Conclusion-CORM-3 and CORM-A1 inhibited thrombosis in vivo, however CORM-A1, which slowly releases carbon monoxide, and displayed a relatively weak hypotensive effect had a more pronounced antithrombotic effect associated with a stronger inhibition of platelet aggregation associated with a decrease in active plasminogen activator inhibitor-1 concentration. In contrast, the fast CO releaser CORM-3 that displayed a more pronounced hypotensive effect inhibited thrombosis primarily through a decrease in fibrin generation, but had no direct influence on platelet aggregation and fibrynolysis. (Arterioscler Thromb Vasc Biol. 2012;32:2149-2157.)Key Words: carbon monoxide ◼ carbon monoxide-releasing molecules ◼ fibrin generation ◼ green fluorescent protein mice ◼ intravital microscopy ◼ plasminogen activator inhibitor-1 ◼ platelet aggregation ◼ rat ◼ thrombosis

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Section: Coagulation Parameters and Fibrin Generationmentioning

confidence: 99%

Antithrombotic Properties of Water-Soluble Carbon Monoxide-Releasing Molecules

Kramkowski

Leszczyńska

Mogielnicki

et al. 2012

ATVB

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“…The assay of overall haemostasis potential (OHP), recently developed by our group [1,2], is a global, simple and time-saving method for determining the combined effects of coagulation and fibrinolysis. The assay is based on the measurement of an area under the fibrin aggregation curve, using citrated plasma samples to which tiny amounts of exogenous thrombin (0.04 IU/ml) and tissueplasminogen activator (t-PA) (330 ng/ml) have been added.…”

Section: Introductionmentioning

confidence: 99%

Identifying hypocoagulable states with a modified global assay of overall haemostasis potential in plasma

Antovič

Blombäck

Sten-Linder

et al. 2005

Blood Coagulation &Amp; Fibrinolysis

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To test the sensitivity of the global assay of overall haemostasis potential (OHP) in detecting hypocoagulation, the OHP was assayed in plasma containing exogenous thrombin (0.04 IU/ml), tissue-plasminogen activator (330 ng/ml), Ca and a platelet reagent. Commercial plasmas with factor II, V, VIII, IX, X, XI, XII or VII deficiency were mixed with normal plasma in different proportions to imitate different severities. Samples from patients with haemophilia and factor XII deficiency were also examined. No clot was found in the absence of factor II/factor X, indicating that the tiny dose of thrombin worked solely as a trigger for the intrinsic pathway activation. Changed levels of the investigated coagulants, apart from factor XII, influenced the outcome. OHPs were decreased in patients with haemophilia but were unchanged or even increased in those with factor XII deficiency. This modified OHP method may therefore be useful for estimating the bleeding tendency in haemophilic patients and to find suitable doses and intervals for prophylactic treatment. It may also be of use in investigations of the effect of antifibrinolytic drugs as well as for identifying a thrombotic tendency in patients with factor XII deficiency. For detection of other coagulation factor deficiencies, our investigations with the commercial plasmas suggest that the OHP assay is also valuable, especially when the intrinsic pathway of the coagulation cascade is impaired.

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“…The coagulation was initiated by thromboplastin, together with exogenous tPA to determine HP and FP [17].…”

Section: Resultsmentioning

confidence: 99%

calix[4]arene C-145 Effects on Plasma Haemostasis

DS¹,

Pashevin²,

LV³

2015

Pharm Anal Acta

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Methods: Parameters of coagulation system of rabbits intravenously injected with C-145 were monitored. We measured thrombin time (TT), ecamulin time (ET) and activated partial thromboplastin time (APTT), determined the levels of fibrinogen, prothrombin, protein C, PAI-1, monitored overall haemostatic potential before and after the injection.Results: C-145 was administrated intravenously in the dose of 7.5 mg/kg of rabbit weight. 4 hours after the injection the thrombin time and activated partial thromboplastin time of rabbit's blood plasma were prolonged 2 and 1,5 times respectively. Such prolongation was observed after 24 hours as well. However, the total fibrinogen and prothrombin levels did not change. Parameters of fibrinolytic system (PAI-1, clot lysis half-time) and anticoagulant system (protein C) remained unchanged.Conclusions: Therefore we assumed that calix[4]arene C-145's effects on haemostasis consisted in selective inhibition of fibrin polymerization and formation of three-dimensional fibrin network.

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A Simple and Rapid Laboratory Method for Determination of Haemostasis Potential in Plasma

Cited by 129 publications

References 10 publications

Antithrombotic Properties of Water-Soluble Carbon Monoxide-Releasing Molecules

Antithrombotic Properties of Water-Soluble Carbon Monoxide-Releasing Molecules

Identifying hypocoagulable states with a modified global assay of overall haemostasis potential in plasma

calix[4]arene C-145 Effects on Plasma Haemostasis

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