A simple and rapid procedure for phytate determination in soybeans and soy products

Kwanyuen, P.; Burton, J. W.

doi:10.1007/s11746-005-1046-9

Cited by 54 publications

(38 citation statements)

References 11 publications

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“…Quantitative HPLC analyses of phytate contents were done according to the method described by Kwanyuen et al [20]. Mass balances for dry matter and phytate were determined.…”

Section: Analyses and Mass Balancesmentioning

confidence: 99%

Fate of Phytic Acid in Producing Soy Protein Ingredients

Deak¹,

Johnson²

2007

J Americ Oil Chem Soc

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Phytic acid (myo-inositol hexaphosphate) is present in soybeans and soy protein products at 1-2% dry matter. Phytate causes poor absorption of essential electrolytes and minerals, and binds to proteins and co-precipitates with isoelectric soy protein isolates. We determined how phytic acid partitioned during different procedures to prepare soy protein ingredients. Procedure and soybean variety significantly affected phytic acid content and recovery. High-sucrose/low-stachyose (HS/LS) soybeans contained significantly (P < 0.05) less phytate than did a typical variety of commodity soybeans (IA2020). In addition, phytate was more readily extracted from the commodity soybeans than from HS/LS soybeans. Among all procedures studied, ethanol-washed soy protein concentrate had the highest phytate contents and yields in the protein products for both soybean varieties (~80 mg/g and 99%, respectively). When protein extraction was carried out at room temperature the protein products had significantly lower phytate yields (60-78%) than when extraction was at 60°C (80-99%). The protein products obtained from normal soybeans had significantly higher phytate contents than the same products made from HS/LS soybeans. When fractionating soy proteins, the glycininrich fraction contained significantly less phytate than the b-conglycinin fraction except for the fractionation procedure performed at room temperature instead of 4°C.

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“…Quantitative HPLC analyses of phytate contents were done according to the method described by Kwanyuen et al [20]. Mass balances for dry matter and phytate were determined.…”

Section: Analyses and Mass Balancesmentioning

confidence: 99%

Fate of Phytic Acid in Producing Soy Protein Ingredients

Deak¹,

Johnson²

2007

J Americ Oil Chem Soc

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“…Therefore, dietary factors that influence the amount of phytate P excreted could potentially alter the WSP fraction of the resultant manures. IP esters analyzed by HPLC (Kwanyuen and Burton, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…with 8 mL of concentrated HNO 3 and 2 mL of 30% H 2 O 2 (vol/vol) with all elements quantified using inductivelycoupled plasma optical-emission spectrometry (4300DV, Perkin-Elmer) detection; and (ii) phytate P was determined by acid extraction followed by HPLC analysis (Agilent HPLC 1100 series, Agilent Technologies, Wilmington, DE; Kwanyuen and Burton, 2005).…”

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confidence: 99%

Interaction of Calcium and Phytate in Broiler Diets. 2. Effects on Total and Soluble Phosphorus Excretion

et al. 2008

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Dietary Ca has been reported to influence the amount of phytate excreted from broilers and affect the solubility of P in excreta. To address the effects of dietary Ca and phytate on P excretion, 12 dietary treatments were fed to broilers from 16 to 21 d of age. Treatments consisted of 3 levels of phytate P (0.10, 0.24, and 0.28%) and 4 levels of Ca (0.47, 0.70, 0.93, and 1.16%) in a randomized complete block design. Feed phytate concentrations were varied by formulating diets with 3 different soybean meals (SBM): a low-phytate SBM, a commercial SBM, and a high phytate Prolina SBM having phytate P concentrations of 0.15 to 0.51%. Fresh excreta was collected from cages during 2 separate 24-h periods; collection I commenced after the start of dietary treatments (16 to 17 d) and collection II followed a 3-d adaptation period (19 to 20 d). Ileal samples were also collected at 21 d. Excreta samples were analyzed for total P, water

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“…Phytate was determined by using the method of Prachuab Kwanyuen and Joe W. Burton (2005). The HPLC system used for eluting phytate consisted of a binary pump, a vacuum micro-degasser for buffers, and an autosampler.…”

Section: Hplc Analysis Of the Samplesmentioning

confidence: 99%

“…Whether hybridization, bioengineering or mutation breeding, it is necessary that the breeders can screen individual plant of low or moderate phytate content effectively at early generations of breeding procedure (Henrik B.P, et al, 2002;Vicky Roslinsky, et al, 2007;Holm Preben B, et al, 2002). Presently, there are many methods on determining phytate content such as HPLC or HPIC (Prachuab Kwanyuen and Joe W. Burton, 2005;Skoghund E, et al, 1998;Chen QC, 2004). Although those means are effective and exact on assaying the phytate contents of food, feed and excretion, they are trivial and low effective on selecting individual plant at early generations because of single grain seed disposal and gigantic samples analyzed.…”

Section: Introductionmentioning

confidence: 99%

Selecting Wheat Seeds of Moderate Phytate Using Colorimetric Method

Li¹,

Wang²,

Gao³

et al. 2011

JAS

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A simple and effective method of determining the phytate content of a single wheat seed was considered necessary for selecting the materials of moderate phytate during early generations breeding. 23 wheat genotypes (16 winter wheat cultivars and 7 spring wheat cultivars) were firstly used as determining samples of phytate content (PC) and inorganic phosphorus content (IPC) of the whole meal, secondarily they were planted in two locations for two times (2004 and 2005). The all single plants were harvested by a plot from those sites, then 6 individual plants were randomly selected to save their seeds for each plot, and the seeds of other plants were grinded into whole meal to assay their PC and IPC. Subsequently, every seed from per individual plant was cut into two semi-grain seeds: the semi-grain seed without embryo (SGSWOE) and the semi-grain seed with embryo (SGSWE); the SGSWOE was carried out to assay colorimetric scoring values by inorganic phosphorus content (CSVIPC), while according to the CSVIPC index, the satisfied SGSWEs were saved to plant at next generation or the dissatisfied SGSWEs were eliminated. The results showed that there was significantly negative correlation between PC and IPC of the wheat meal from either the first samples or the materials harvested at those sites, and for the latter there was significantly negative correlation between PC and CSVIPC. There was no significant difference of CSVIPC among the SGSWEOs and SGSWE of the spikes and individual plants from the same plot or the same genotype at any sites. In conclusion the CSVIPC of the SGSWOEs can indirectly express PC level of the wheat meal: the higher the former was, the lower the latter was. So the CSVIPC of SGSWOE can be applied to assaying the level of corresponding SGSWE according to their index, and screening the individual plant of moderation phytate. Moreover, this method is characterized by high efficiency during breeding processing.

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A simple and rapid procedure for phytate determination in soybeans and soy products

Cited by 54 publications

References 11 publications

Fate of Phytic Acid in Producing Soy Protein Ingredients

Fate of Phytic Acid in Producing Soy Protein Ingredients

Interaction of Calcium and Phytate in Broiler Diets. 2. Effects on Total and Soluble Phosphorus Excretion

Selecting Wheat Seeds of Moderate Phytate Using Colorimetric Method

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