A simple and rapid ultra‐high‐performance liquid chromatography–tandem mass spectrometry method to determine plasma biotin in hemodialysis patients

Abstract

A simple, rapid, and selective method for determination of plasma biotin was developed using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). After single-step protein precipitation with methanol, biotin and stable isotope-labeled biotin as an internal standard (IS) were chromatographed on a pentafluorophenyl stationary-phase column (2.1 × 100 mm, 2.7 μm) under isocratic conditions using 10 mm ammonium formate-acetonitrile (93:7, v/v) at a flow rate of 0.6 mL/min. The total chromatographic runtime was 5 min for each injection. Detection was performed in a positive electrospray ionization mode by monitoring selected ion transitions at m/z 245.1/227.0 and 249.1/231.0 for biotin and the IS, respectively. The calibration curve was linear in the range of 0.05-2 ng/mL using 300 μL of plasma. The intra- and inter-day precisions were all <7.1%. The accuracy varied from -0.7 to 8.2%. The developed UHPLC-MS/MS method was successfully applied to determine plasma biotin concentrations in hemodialysis patients. Copyright © 2016 John Wiley & Sons, Ltd.

Clinical risk assessment of biotin interference with a high-sensitivity cardiac troponin T assay

“…A limitation of the study was that the samples used were not specifically collected for the purpose of measuring biotin levels. Prior studies have shown that biotin is stable under such conditions [42]. Our data reflect biotin distribution in the intended use population for troponin testing, but might differ from broader or more selected populations.…”

Quantitative measurement of biotin by LC-MS/MS in human serum and the effect of multivitamin and renal function on biotin concentration

“…Usually biotin absorption and its renal excretion are so fast, its half-life is 8 to 16 hours (15,16). However, patients with decreased renal function will excrete the biotin inefficiently and serum biotin levels are known to be elevated (17,18). Elevated concentrations of biotin and its metabolites were associated with muscle cramp of hemodialysis patient (18)(19)(20).…”

“…However, patients with decreased renal function will excrete the biotin inefficiently and serum biotin levels are known to be elevated (17,18). Elevated concentrations of biotin and its metabolites were associated with muscle cramp of hemodialysis patient (18)(19)(20). In Seoul National University Hospital, some multivitamins contain biotin between 22.5 μg and 300 μg.…”

Plasma Levels of Biotin Metabolites Are Elevated in Hemodialysis Patients with Cramps

“…In the new UHPLC-MS/MS method, the necessary detection limit of biotin separately was 0.05 μg/l. In the plasma of HD patients, the pentafluorophenyl stationary phase column was applied to avoid a strong matrix effect and many interference peaks in the biotin region (Yagi et al 2016). However, with that method we have not yet been able to determine metabolites selectively, such as bisnorbiotin, biotin sulfoxide and biotin sulfone in the plasma of HD patients.…”

“…Their method, however, had the drawback of requiring many procedures, and was unsuitable for multiple measurements. Recently, we have successfully developed a simple ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method to quantify plasma biotin of HD patients with sufficient sensitivity (Yagi et al 2016). In the present study, we applied UHPLC-MS/MS and ELISA to determine the plasma levels of biotin and TABS, respectively, in HD patients and healthy subjects.…”