A Simple and Rapid UPLC-PDA Method for Quality Control of Nardostachys jatamansi

Abstract

is a well-documented herbal agent used to treat digestive and neuropsychiatric disorders in oriental medicinal systems. However, few simple, rapid, and comprehensive methods were reported for quality assessment and control of . Herein, a UPLC with photodiode array detection method was developed for both fingerprint investigation of and simultaneous quantitative analysis of the six serotonin transporter modulatory constituents in . For chromatographic fingerprinting, 24 common peaks were selected as characteristic peaks to assess the consistency of samples from different retail sources. Six of the common peaks (5, 7, 12: , and 16: - 18: ) were identified as desoxo-narchinol A, buddleoside, isonardosinone, nardosinone, kanshone H, and (-)-aristolone, respectively, by phytochemical investigation. Five of the six compounds significantly either enhanced or inhibited serotonin transporter activity, while (-)-aristolone (18: ) didn't show any serotonin transporter activity. In quantitative analysis, the six compounds showed good linearity (> 0.999) within test ranges. The precision, expressed as relative standard deviation, was in the range of 0.25 - 2.77%, and the recovery of the method was in the range of 92 - 105%. The UPLC-photodiode array detection-based fingerprint analysis and quantitative methods reported here could be used for routine quality control of .

Degradation Profiling of Nardosinone at High Temperature and in Simulated Gastric and Intestinal Fluids

“…Then, the mixture was subjected to an ODS reversed-phase column eluted with gradient MeOH-H 2 O (30:70, 40:60, 50:50, 60:40, 70:30, 85:15, and 100:0 for 5, 5, 30, 13, 27, 4, and 10 column volumes, respectively, v/v) to obtain 9 fractions (Fr. [1][2][3][4][5][6][7][8][9]. Fraction 4 from the 50% MeOH elution, viz., 2-deoxokanshone M (1), was obtained (5.773 g) with a yield of 64.23%.…”

“…Nardostachys jatamansi DC., which has been extensively used for centuries in Himalayan countries to treat neurological and cardiovascular diseases [1,2], presents bioactive constituents such as nardosinone, nardosinonediol, isonardosinone, desoxo-narchinol A, and aristolone, which play crucial roles in the plant's therapeutic properties [2]. Among these constituents, nardosinone is integral for the authentication and quality evaluation of N. jatamansi [3][4][5], and it is stipulated to constitute at least 0.1% of the dried processed roots and rhizomes of the plant, according to the Chinese Pharmacopoeia [6]. Despite a plethora of pharmacological benefits of nardosinone, including anti-inflammatory [7], antidepressant [8], cardioprotective [9], anti-neuroinflammatory [10], anti-arrhythmic [11], and other potential pharmacological effects [12][13][14][15], its practical application as a clinical candidate drug is still limited.…”

Analysis and subsequent molecular docking of selected phytochemicals with SLC6A3 and SLC6A4 as potential therapeutic agents for Obsessive-Compulsive Disorder (OCD)

“…In continuation to the previous study, there is an attempt to screen out the potentially useful phytochemicals against this moodomics disorder. Several potentially useful phytochemicals are known to associate with SLC6A3 and SLC6A4, which have been identified through the text mining of various publications documenting the association between known phytochemicals, dopamine transporter and serotonin transporter [9][10] [11][12] [13][14] [15]. As the first step, a list of phytochemicals is prepared by going through various papers and then their canonical SMILES line notations are retrieved from PubChem Compund, which are used for calculating the molecular properties of these phytochemicals through the use of Molinspiration, followed by selection of the ones suitable as drug candidates using Lipinski's Rule of five and finally, the ones which are selected have their best docking configurations identified using AutoDock v4.2.6 in order to obtain potential therapeutics for OCD patients [16][17].…”

Nardostachys chinensis Batalin: A review of traditional uses, phytochemistry, and pharmacology

“…There is an increasing trend in number of research papers produced on N. chinensis. Recently, a number of studies have been conducted on pharmacology and phytochemistry of N. chinensis (Jin, Xiao, Qun, Li, & Liu, 2018;Kapoor, Yadav, Chopra, Mahapatra, & Agrawal, 2017;Ko et al, 2018;Le et al, 2018;Liu et al, 2018;Rehman et al, 2017;Shen et al, 2017;Shen et al, 2018;Song et al, 2018;Zhang et al, 2018).…”