A simple and reliable analytical method for simultaneous quantification of first line antitubercular drugs in human plasma by LCMS/MS

Abstract

The present study describes the optimization of a simple and reliable method for the determination of four first line antitubercular drugs in human plasma. The studied analytes were Isoniazid (H),...

A high‐throughput LC–MS/MS method for simultaneous determination of isoniazid, ethambutol and pyrazinamide in human plasma

“…Our previous study showed that the efficacy of phospholipid removal is more than 95%. 24 Compared with previously published studies, [12][13][14][15][16][17][18][19][20][21][22] our method provides a short analysis time and an efficient clean-up sample procedure which prolongs the column lifetime. The quantification range is enough to quantify selected drugs after their therapeutical doses.…”

“…Nowadays, the combination of liquid chromatography with tandem mass spectrometry (LC–MS/MS) presents a powerful and sensitive technique to perform TDM on a large number of samples. Several LC–MS/MS methods have been published for simultaneously quantifying anti‐TB drugs in human plasma 12–22 . However, these methods lack good separation and/or peak shape, and suffer from disadvantages like long run time, large sample volume, low sensitivity, and time‐consuming and strenuous sample preparation.…”

“…Several LC–MS/MS methods have been published for simultaneously quantifying anti‐TB drugs in human plasma. 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 However, these methods lack good separation and/or peak shape, and suffer from disadvantages like long run time, large sample volume, low sensitivity, and time‐consuming and strenuous sample preparation.…”

“…Blood was collected in a tube with potassium EDTA as an anticoagulant, centrifuged at 2500 Â g for 15 min at room temperature and stored at À20 C until analysis. RESULTS AND DISCUSSION3.Method development and LC-MS/MS optimizationSeveral LC-MS/MS-based methods have been developed to quantify first-line anti-TB drugs, including isoniazid, ethambutol and pyrazinamide, in human plasma,[12][13][14][15][16][17][18][19][20][21][22] but not all of them are suitable for application in routine and high-throughput TDM. The main limitations of these studies are poor chromatographic separation of analytes, large sample volume requirements, inadequate concentration range, laborious sample preparation and thus low throughput of analysis.…”

Pyrazinamide-induced Polyarthralgia and Myalgia in a Case of Stable Chronic Kidney Disease

“…The plasma concentrations were estimated using a simple and validated liquid chromatography-tandem mass spectrometry (LCMS/MS) in the Department of Pharmaceutical Chemistry, Poona College of Pharmacy, Pune, India, developed for simultaneous measurement of all the first-line antitubercular drugs. 5 Suspecting drug-induced polyarthralgia and myopathy, pyrazinamide was discontinued and replaced with levofloxacin 500 mg/d as per NTEP guidelines. 6 Febuxostat dose was increased to 80 mg/d and ibuprofen 400 mg/d for 5 days was added in her drug chart.…”