1978
DOI: 10.1099/00222615-11-4-541
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A Simple and Safe Volumetric Alternative to the Method of Miles, Misra and Irwin for Counting Viable Bacteria

Abstract: Radclife Infirmary, Oxford THE method of Miles, Misra and Irwin (1938) has for many years been the standard technique for the determination of viable bacterial counts by surface inoculation of solid media. However, this method requires precision and skill if accurate results are to be obtained. One problem is the difficulty of preparing pipettes that accurately and consistently deliver 50 drops per ml.The method is also not without hazard to the operator. Johannson and Ferris (1946) have demonstrated aerosol… Show more

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Cited by 14 publications
(7 citation statements)
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“…Each swab was then vortexed in 1 mL of neutralizing broth (Difco) and serial dilutions of 1:10, 1:100, and 1:1000 were prepared. SDA plates were inoculated according to Miles and Misra's method [16]. Each test was carried out in triplicate.…”
Section: Efficacy Of Hospital Disinfectants In Clearance Of C Aurismentioning
confidence: 99%
“…Each swab was then vortexed in 1 mL of neutralizing broth (Difco) and serial dilutions of 1:10, 1:100, and 1:1000 were prepared. SDA plates were inoculated according to Miles and Misra's method [16]. Each test was carried out in triplicate.…”
Section: Efficacy Of Hospital Disinfectants In Clearance Of C Aurismentioning
confidence: 99%
“…These were isolated from the rhizosphere of industrial chicory plants (Cichorium intybus L.), capable of solubilizing 117.32 mg L −1 P in vitro [26]. Strains were multiplied using the methodology, as described by Slack and Wheldon [27], which, consisted in reproducing in standard nutrient broth I (Merck) under constant agitation at 150 rpm and 25 • C for two days (Lab Companion model SI-600 benchtop shaker; Lab Companion, Jeio Tech's laboratory instrument brand, Daejeon, South Korea). In order to determine the concentration, the optical density was measured in a spectrophotometer (MECASYS, POP Optizen Bio, Daejeon, South Korea) at 600 nm and its equivalence in CFU mL −1 was calculated.…”
Section: Origin Of the Strain And Inoculum Preparationmentioning
confidence: 99%
“…They were then filtered and the P determined by colorimetry with nitro-vanado-molybdate at 720 nm [27]. Olsen-P was extracted with Na 2 CO 3 0.5 mol L −1 , pH 8.5, and also determined by colorimetric method at 820 nm.…”
Section: Analysis Of P In the Plants And Soil And Ph Levelsmentioning
confidence: 99%
“…The model blood samples were inoculated onto agar plates using a modified version of the method described by Miles and co-workers [24][25][26]. Ten-fold serially diluted (10 1 -10 7 ) blood samples in 0.9% sterile NaCl were dropped onto seven sections (15 ml per section) per agar plate (90 mm Petri dishes).…”
Section: Evaluation Of Culture Mediamentioning
confidence: 99%