A simple and sensitive immunoassay for the determination of human chorionic gonadotropin by graphene-based chemiluminescence resonance energy transfer

A facile and sensitive peptide-modulating graphene oxide nanoribbon catalytic nanoplasmon analytical platform for human chorionic gonadotropin

“…We can see that the RRS analytical system is more sensitive than the Abs and was thus chosen for sample detection. Compared to the reported hCG methods, [25][26][27][28][29][30][31][32][33][34][35][36] this RRS method is not only sensitive and selective but also facile, of low-cost and without nanoparticle aggregation. The clinical method of chemiluminescent immunoassay had a DL of 0.143 ng/mL, limit of quantitation (LQ) of 5 ng/mL and linear range of 5-540 ng/mL hCG; 37 the DL and LQ of this RRS method are better than the clinical method, and the linear range of the two methods are close to each other, as the upper LQ divided by the lower LQ is 108 and 100, respectively.…”

“…Recently, some new assays were developed for the detection of hCG. [31][32][33][34][35][36] Zhao et al 31 reported a microplate magnetic chemiluminescence enzyme immunoassay for the determination of hCG using the alkaline phosphatase (ALP) as a labeled reagent and 3-(2′-spiroadamantane)-4-methoxy-4-(3″-phosphoryloxy) phenyl-1,2-dioxetane (AMPPD) as a chemiluminescence reagent. Chang et al 32 combined catalytic gold nanoparticles with an hCG-specific peptide aptamer to create a simple, sensitive, label-free colorimetric assay for hCG.…”

“…Ding and Yang 33 reported an antibody-free and label-free mechanism for detecting hCG using a short oligopeptide as an hCG receptor to bind hCG, with a DL of 2 nmol/L hCG. Lei et al 34 reported a strategy of chemiluminescence resonance energy transfer for detection of 0.12 nmol/mL hCG using graphene as an efficient long-range energy acceptor and the magnetic nanoparticles for magnetic separation and immobilization of HRP-labeled anti-hCG antibody. Chen et al 35 reported an immunomagnetic assay for quantitative analysis of total β-subunit of hCG concentration in urine.…”

Electrochemical biosensors for hormone analyses

“…It is secreted by the anterior pituitary and regulated milk production in the mammary glands and the synthesis of progesterone in the corpus luteum. Serafin et al μIU/mL Lin et al (1996) hCG GCE/Fe 3 O 4 nanoparticles/HRP-labeled anti-hCG/hCG/anti-hCG antibody conjugated graphene Antibody 0.1-10 mIU/mL 0.06 mIU/mL Lei et al (2014) developed a novel electrochemical immunosensor for the determination of prolactin hormone (PRL) by using glassy carbon electrode as a transducer that was modified with a poly(pyrrolepropionic acid)/multiwalled carbon nanotubes hybrid nanomaterials. Anti-PRL labeled with alkaline phosphatase (AP) was reacted with PRL in the sample.…”

Chemiluminescence resonance energy transfer imaging on magnetic particles for single-nucleotide polymorphism detection based on ligation chain reaction

“…Due to the emission of light caused by a chemical reaction without an external excitation source, CL has virtually nonspecific signals and is extremely sensitive with simple instrumental set-up. Chemiluminescence resonance energy transfer (CRET) occurs via nonradiative dipole-dipole energy transfer between a CL donor and a suitable acceptor molecule that are in close proximity without the need of a light source (Lei et al, 2014;Chen and Li, 2014;Mun et al, 2014). The CRET signal can be easily readout by photometers or luminometers, especially by sensitive imaging photon detector of cooled low-light charge-coupled device (CCD) for chemiluminescence imaging assay (Bi et al, 2013;Zong et al, 2012).…”