A Simple Assay for Measuring Catalase Activity: A Visual Approach

Iwase, Tomoyuki; Tajima, Akiko; Sugimoto, Shinya; Okuda, Ken Ichi; Hironaka, Ippei; Kamata, Yuko; Takada, Koji; Mizunoe, Yoshimitsu

doi:10.1038/srep03081

Cited by 246 publications

(191 citation statements)

References 22 publications

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“…Catalase activity was measured as previously described described (Iwase et al, 2013). Briefly, HEK cultures expressing the different plasmids were washed with PBS, detached by trypsinization and centrifuged (800 g – 5 min).…”

Section: Methodsmentioning

confidence: 99%

Nrf2 stabilization prevents critical oxidative damage in Down syndrome cells

Zamponi

Coşkun

et al. 2018

Aging Cell

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SummaryMounting evidence implicates chronic oxidative stress as a critical driver of the aging process. Down syndrome (DS) is characterized by a complex phenotype, including early senescence. DS cells display increased levels of reactive oxygen species (ROS) and mitochondrial structural and metabolic dysfunction, which are counterbalanced by sustained Nrf2‐mediated transcription of cellular antioxidant response elements (ARE). Here, we show that caspase 3/PKCδdependent activation of the Nrf2 pathway in DS and Dp16 (a mouse model of DS) cells is necessary to protect against chronic oxidative damage and to preserve cellular functionality. Mitochondria‐targeted catalase (mCAT) significantly reduced oxidative stress, restored mitochondrial structure and function, normalized replicative and wound healing capacity, and rendered the Nrf2‐mediated antioxidant response dispensable. These results highlight the critical role of Nrf2/ARE in the maintenance of DS cell homeostasis and validate mitochondrial‐specific interventions as a key aspect of antioxidant and antiaging therapies.

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Section: Methodsmentioning

confidence: 99%

Nrf2 stabilization prevents critical oxidative damage in Down syndrome cells

Zamponi

Coşkun

et al. 2018

Aging Cell

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“…AT activity was determined according to the methodology described by Iwase et al (2013). In this analysis, 100 µL of sample or standard were incubated with 100 µL of 1% Triton-X and 100 µL of 30% of hydrogen peroxide.…”

Section: Methodsmentioning

confidence: 99%

Catalase, superoxide dismutase, glutathione peroxidase and oxygen radical absorbance capacity in the gut of juvenile pacu Piaractus mesopotamicus and dourado Salminus brasiliensis fed bovine first milk secretion

Moretti¹,

Nordi²,

Cruz³

et al. 2017

lajar

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ABSTRACT. Besides the immunological benefits of colostrum consumption, this lacteal secretion has a great concentration of biological molecules that can affect gut physiology and fish healthy. This study evaluated the antioxidant potential in the gut of juvenile pacu, Piaractus mesopotamicus, and dourado, Salminus brasiliensis, fed twice a day with diets containing 0, 10 or 20% of lyophilized bovine colostrum (LBC). The whole medium and posterior intestine was collected after 30 or 60 experimental days and the activity of catalase, superoxide dismutase and glutathione peroxidase was determined as one international unit per milligram of protein, and the oxygen radical absorbance capacity as µM of equivalent Trolox per milligram of protein. Only the juvenile pacu were affected by the diet containing LBC (P < 0.05). The juvenile fed 10% LBC showed higher oxygen absorbance capacity than the juveniles fed 20% LBC (P < 0.05). Interaction between diet and period was observed to superoxide dismutase (SOD) activity (P < 0.05), juvenile pacu fed 0% LBC and 10% LBC did not change enzyme activity at 30 and 60 days, whereas juvenile fed 20% LBC showed higher value at 60 days compared to 30 days (P < 0.05). The present result reveal that the consumption of diet containing LBC improved SOD activity in the gut of juvenile pacu indicating a possible protective action of this lacteal secretion in an omnivorous fish.

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“…Catalase activity: The isolate possess catalase, when a small amount of fungal isolate is added to hydrogen peroxide, bubbles of oxygen are observed according to the method of Tadayuki et al [29].…”

Section: Enzyme Analysismentioning

confidence: 99%

Impact of Enzymes and Toxins Potentiality of Four Aspergillus Species to Cause Aspergillosis

Zohri¹,

Aboul-Nasr²,

Adam³

et al. 2017

Biol Med

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Aspergillus species are the main causing agents of invasive apergillosis chest disease. Eighty isolates of Aspergillus species, A. flavus (20), A. fumigatus (15), A. niger (30) and A. terreus (15 isolates), previously isolated and identified from aspergillosis suspected patients at our lab in Assiut university hospitals, were assayed for their enzymes and toxins profile. The results revealed that, all of the tested isolates were able to utilize calf lung tissue and produce catalase and peroxidase enzymes. Meanwhile, 82.5-90% of the fungal isolates had the ability to produce protease, lipase, urease and phospholipase, whereas, 70% of isolates exhibited hemolytic activities. Thin layer chromatography (TLC) of the cleaned extracts of the tested isolates exhibited the ability of all A. flavus isolates assayed to produce aflatoxins B1 and G1, 53%, moreover, A. fumigatus isolates produced fumagillin and gliotoxin. On the other side, 43.3% and 23.3% of A. niger isolates produced ochratoxins and gliotoxin respectively. Virulence assay of 10 µl of gliotoxin standard and cleaned extracts of A. fumigatus toxic isolates showed necrotic area on Guinea Pigs lungs compared to the control. Thus, opportunistic fungi isolated from aspergillosis patients possess high enzymatic and toxic profile that might play an important role in their mycopathy.

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A Simple Assay for Measuring Catalase Activity: A Visual Approach

Cited by 246 publications

References 22 publications

Nrf2 stabilization prevents critical oxidative damage in Down syndrome cells

Nrf2 stabilization prevents critical oxidative damage in Down syndrome cells

Catalase, superoxide dismutase, glutathione peroxidase and oxygen radical absorbance capacity in the gut of juvenile pacu Piaractus mesopotamicus and dourado Salminus brasiliensis fed bovine first milk secretion

Impact of Enzymes and Toxins Potentiality of Four Aspergillus Species to Cause Aspergillosis

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