A simple chromatographic technique for the estimation of urinary levels of normetadrenaline and metadrenaline

Hanson, Paul R.; Smith, Joanna; Robinson, R.; Stott, A.W.

doi:10.1016/s0009-9120(80)80006-3

Cited by 3 publications

(4 citation statements)

References 18 publications

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“…31,42 Individuals with pheochromocytoma would be expected to have markedly elevated NME concentrations. 12,42 Concentrations of metanephrines can be measured using high-performance liquid chromatography (HPLC), spectrophotometry, mass spectrometry, and radioimmunoassay in human samples 5,19,24,33,39,41 as well as in canine samples. 6,14,17,26,27,34,37 In feline samples, chromatographic methods have been used to measure metanephrines in plasma 42 and urine.…”

Section: Discussionmentioning

confidence: 99%

“…Compared to catecholamines, analysis of metanephrines may be preferable because the metabolites are more stable and can be found in higher concentrations in human plasma and urine as well as in canine urine samples. 12,19,26,27,30 In people and dogs, concentrations of catecholamines and metanephrines can be measured in both plasma and urine samples. 10,12,15,22,23,26,27,34,37,39 In cats, few studies are available on analysis of catecholamine and metanephrine concentrations in blood and urine.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of an ELISA for metanephrines in feline urine

et al. 2018

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Catecholamines can be used to evaluate neuroendocrine tumors, stress, and potentially pain, but catecholamines degrade rapidly. Their metabolites normetanephrine (NME) and metanephrine (ME) have better stability in urine. In cats, urine sampling in a home environment would be beneficial to reduce effects of clinical stress and simplify sampling. We evaluated a human urine ELISA for analysis of NME and ME in feline urine, and investigated the effects of acidification, cat tray pellets, and storage time at room temperature up to 8.5 h. In 26 feline urine samples, mean NME concentration was 192 ± 80 ng/mL, mean intra- and inter-assay CV was 6.5% and 4.2%, respectively, and spike recovery was 98-101%, but dilutional recovery was unsatisfactory. For ME, mean intra- and inter-assay CV was 10.2% and 4.1%, respectively. Mean urine ME concentration was 32.1 ± 18.3 ng/mL, close to the kit's lowest standard, and spike recovery was 65-90%; the ELISA could not be validated for ME. The stability study, performed for NME on 12 urine samples, did not identify differences between acidified and non-acidified samples, cat tray pellets, or storage time, and no interaction effects. The ME ELISA was not suitable for feline urine; performance of the NME ELISA was acceptable, except for dilution recovery. For analysis of NME, feline urine can be sampled at home using cat tray pellets and stored at room temperature up to 8.5 h without acidification.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of an ELISA for metanephrines in feline urine

et al. 2018

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“…8,20 These metabolites, collectively called metanephrines, are stable and have been found in higher concentrations than catecholamines in canine urine, as well as in human plasma and urine. 10,15,16,19,30 However, acidification of urine samples is still recommended by manufacturers of assays for the metabolites.…”

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Quantification of normetanephrine in canine urine using ELISA: evaluation of factors affecting results

et al. 2021

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Catecholamine release increases in dogs with pheochromocytomas and in situations of stress. Although plasma catecholamines degrade rapidly, their metabolites, normetanephrine (NME) and metanephrine (ME), are stable in acidified urine. Our aim was to verify a human urine ELISA kit for the quantification of NME and ME in canine urine and to determine the effects on metabolite stability of sampling time (morning or midday) and day (ordinary or day spent in a clinic). We analyzed 179 urine samples from 17 healthy dogs. For NME, the mean intra-assay CV was 6.0% for all samples and 4.3% for the canine control; inter-assay CVs were 3.3, 3.8, and 12% for high and low concentration human urine positive controls supplied in the ELISA kit and a positive canine control, respectively; spike-recovery was 90–101%. For ME, mean intra-assay CV was 6.5% for samples and 9.0% for the canine control; inter-assay CVs were 12.7, 7.2, and 22.5% for high and low concentration human urine positive controls supplied in the ELISA kit and a positive canine control, respectively; spike-recovery was 85–89%. Dilution recovery was unsatisfactory for both metabolites. Based on our verification results, NME was selected for remaining analyses. We found no effect on NME concentrations of acidification or room temperature storage for up to 24 h. The NME:creatinine ratio was higher after the first of 3 clinic days compared to the same morning (111.2 ± 5.5 vs. 82.9 ± 5.3; p < 0.0001), but not on the other days. NME verification results were generally superior to ME. Dilution studies were unsatisfactory for both metabolites. Given that NME was stable without acidification at room temperature, urine samples can be collected at home. The clinic environment can cause higher NME:creatinine ratios, especially in unaccustomed dogs.

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Anwendung der HPLC in der Klinisch-chemischen Analytik

Schöneshöfer

1989

Analytiker-Taschenbuch

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A simple chromatographic technique for the estimation of urinary levels of normetadrenaline and metadrenaline

Cited by 3 publications

References 18 publications

Evaluation of an ELISA for metanephrines in feline urine

Evaluation of an ELISA for metanephrines in feline urine

Quantification of normetanephrine in canine urine using ELISA: evaluation of factors affecting results

Anwendung der HPLC in der Klinisch-chemischen Analytik

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