A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses

Russell, W. C.; Newman, C.E.; Williamson, D. H.

doi:10.1038/253461a0

Cited by 757 publications

(261 citation statements)

References 4 publications

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“…In view of these findi tgs, it is therefore essential to exclude Mycoplasma infection hen evaluating putative atypical angiotensin binding sites on altured eukaryotic cells. Accordingly, the IEC-18 cells used in 1 le present study were free of such contamination as assessed 1-y staining with 4'-6-diamidino-2'-phenylindole [17] (data not lown). Atypical angiotensin binding sites have also been described ~n chick embryo chorioallantoic membrane [23] and turkey ~drenal gland [24], as well as oocytes [25] and cardiac memtranes [26] isolated from Xenopus laevis.…”

Section: Discussionmentioning

confidence: 79%

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Identification of atypical (non‐AT₁, non‐AT₂) angiotensin binding sites with high affinity for angiotensin I on IEC‐18 rat intestinal epithelial cells

Smith

1995

FEBS Letters

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Section: Discussionmentioning

confidence: 79%

“…Cells were used after 3-4 days when they formed confluent monolayers. Cultures were free from contamination with Myeoplasma as determined by staining with 4'-6-diamidino-2'-phenylindole [17].…”

Section: ~ In~oducfionmentioning

confidence: 99%

Identification of atypical (non‐AT₁, non‐AT₂) angiotensin binding sites with high affinity for angiotensin I on IEC‐18 rat intestinal epithelial cells

Smith

1995

FEBS Letters

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“…Antibiotic-free media testing and microbiological culture using tryptose soy broth and fluid thioglycollate medium were performed to test for the presence of aerobic, anaerobic, facultative anaerobic bacteria and fungi. A modified DIPA (4′,6-diamidino-2′-phenylindole) staining method according to (Russell et al 1975) was conducted to test for Mycoplasma spp. Briefly, prior to detection, T-YFP-SF1 and YFP-SF1 cells were cultured in antibiotic-free media for at least 1 wk or a minimum of two passages.…”

Section: Methodsmentioning

confidence: 99%

Establishment and characterization of fibroblast cell lines from the skin of the Yangtze finless porpoise

Wang

Nie

et al. 2011

In Vitro Cell.Dev.Biol.-Animal

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The Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis), as the sole freshwater subspecies of N. phocaenoides, is endemic to the Yangtze River and its adjacent lakes. Its population has declined significantly over recent decades. In this study, we established a skin-derived finite fibroblast cell line of the Yangtze finless porpoise, named YFP-SF1, using primary cell culture methods, and an immortalized cell line, T-YFP-SF1, through co-transfection (GFP and SV40 T antigens) techniques. YFP-SF1 proliferated continuously with a minimum population doubling time of 31 h and exhibited age-dependent changes in growth rate. T-YFP-SF1 cells exhibited fibroblast morphology and were characterized by a shorter doubling time, higher attachment efficiencies, colony formation at a low seeding density, and growth in low serum concentrations. Anchorage independence and foci formation in the cell monolayer were observed from passage 36. The chromosome number of YFP-SF1 and T-YFP-SF1 remained stable at 2n=44 in the early passages, and the viability of thawed cells remained above 90% after cryopreservation in liquid nitrogen. Taken together, we have established fibroblast cell lines of Yangtze finless porpoise for the first time, which might assist as an in vitro model for this endangered mammal.

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“…After two washings with I ml of cold HBS, cells were incubated with HBS containing protcase inhibitors for 5 min at 0°C and then treated with BSOCOES as described above. Cells were counted on lysates by using 4',6-diamidine-2'-phenylindole dihydrochloride (DAPI; Boehringer, Mannheim, Germany) [39]. Briefly, 5 ~1 of cell lysates were mixed with I.5 ml of IO mM Tris-HCI, IO mM EDTA, 100 mM sodium chloride, 0.05% Triton X-100, 100 @ml DAPI, pH 7.0, and fluorescence was measured.…”

Section: Tetanus Toxin Cell Binding Umd Cross-linkingmentioning

confidence: 99%

Tetanus toxin receptor Specific cross‐linking of tetanus toxin to a protein of NGF‐differentiated PC 12 cells

Schiavo¹,

Ferrari

Rossetto³

et al. 1991

FEBS Letters

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. In the presence of protein cross-linking agents, ['*'l]tetanus toxin, bound to these cells at O'C, forms a cross-linked product with apparent molecular weight of 120 kDa. The formation of ['*'I]tetanus toxin conjugate involves the heavy chain of the toxin, is prevented by cold toxin and it is largely reduced by pretreating cells with proteases. The cross-linked product is formed only upon incubation of the toxin with NGF-differentiated cells. These results suggest that a protein with apparent molecular weight of 20 kDa is involved in the neurospecific binding of tetanus toxin.

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A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses

Cited by 757 publications

References 4 publications

Identification of atypical (non‐AT₁, non‐AT₂) angiotensin binding sites with high affinity for angiotensin I on IEC‐18 rat intestinal epithelial cells

Identification of atypical (non‐AT₁, non‐AT₂) angiotensin binding sites with high affinity for angiotensin I on IEC‐18 rat intestinal epithelial cells

Establishment and characterization of fibroblast cell lines from the skin of the Yangtze finless porpoise

Tetanus toxin receptor Specific cross‐linking of tetanus toxin to a protein of NGF‐differentiated PC 12 cells

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A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses

Cited by 757 publications

References 4 publications

Identification of atypical (non‐AT1, non‐AT2) angiotensin binding sites with high affinity for angiotensin I on IEC‐18 rat intestinal epithelial cells

Identification of atypical (non‐AT1, non‐AT2) angiotensin binding sites with high affinity for angiotensin I on IEC‐18 rat intestinal epithelial cells

Establishment and characterization of fibroblast cell lines from the skin of the Yangtze finless porpoise

Tetanus toxin receptor Specific cross‐linking of tetanus toxin to a protein of NGF‐differentiated PC 12 cells

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Identification of atypical (non‐AT₁, non‐AT₂) angiotensin binding sites with high affinity for angiotensin I on IEC‐18 rat intestinal epithelial cells

Identification of atypical (non‐AT₁, non‐AT₂) angiotensin binding sites with high affinity for angiotensin I on IEC‐18 rat intestinal epithelial cells