A simple enzymatic microdetermination of cytochrome b5 in erythrocytes

Takeshita, M; Yubisui, Toshitsugu; Tanishima, Kiyoh; Yoneyama, Yoshimasa

doi:10.1016/0003-2697(80)90387-5

Cited by 2 publications

(3 citation statements)

References 16 publications

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“…The separated erythrocytes were stored on ice before the next treatment. Cytochrome b5 and cytochrome b5 reductase in isolated erythrocytes were stable for at least 14 days and 25 days respectively at 40C (Takeshita et al, 1980;Tanishima et al, 1980).…”

Section: Separation Methodsmentioning

confidence: 98%

“…In erythrocytes from hereditary methaemoglobinaemic patients there has been no proof that cytochrome b5 does not exhibit abnormality either in content or in ability to transfer electrons from cytochrome b5 reductase to methaemoglobin. We first developed an assay method for cytochrome b5 content of erythrocytes (Takeshita et al, 1980) and applied it to the study of methaemoglobinaemia.…”

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confidence: 99%

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Age-dependent decay of cytochrome b5 and cytochrome b5 reductase in human erythrocytes

Matsuki¹,

Tamura²,

Takeshita³

et al. 1981

Biochemical Journal

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Age-dependent decrease in cytochrome b5 was observed in erythrocytes from both a normal person and a patient with hereditary methaemoglobinaemia without neurological symptoms. With aging, concentrations of cytochrome b5 in erythrocytes from the patient were almost the same as those in the control. Age-dependent decrease in cytochrome b5 reductase activity in the control erythrocytes was also shown; however, the reductase activity was very low in erythrocytes from the patient over the whole age range. Our studies show that methaemoglobin content of erythrocytes seems to be dependent on the content of cytochrome b5 in the cells, both in the control subject and in the patient.

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Section: Separation Methodsmentioning

confidence: 98%

mentioning

confidence: 99%

Age-dependent decay of cytochrome b5 and cytochrome b5 reductase in human erythrocytes

Matsuki¹,

Tamura²,

Takeshita³

et al. 1981

Biochemical Journal

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“…Cytochrome b5 content was determined using the method of Takeshita et al . (1980). The standard assay for GSH content was carried out under conditions similar to those described by Smith & Anderson (1992).…”

Section: Methodsmentioning

confidence: 99%

Survival physiology and sex ratio of the Chinese white pine beetleDendroctonus armandi(Coleoptera: Scolytinae) during host colonization and overwintering

Dai

Zheng

Wang

et al. 2019

Bull. Entomol. Res.

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The Chinese white pine beetle Dendroctonus armandi (Coleoptera: Scolytinae) typically displays bivoltinism at altitudes below 1700 m in the Qinling Mountains, China. The periods of host colonization and larval overwintering are two important phases in the life cycle of bark beetles, as it is during these periods that they have to contend with host plant defences and periods of intense cold, respectively. Although during different seasons, the females and males of Chinese white pine beetles show varying tolerances to host plant terpenoids, the sex ratio and survival physiology condition of the two beetle generations are unknown. We investigated the sex ratio of individuals, and also examined the body mass, energy stores, and detoxication enzymes of males and females in each of the two generations in order to determine the overall population stability of each generation. We identified a female-biased sex ratio among adults in both generations. Furthermore, patterns of body mass, energy stores, and detoxication enzymes were found to differ between the two sexes and two seasons. Compared with the males, the females have a larger body mass and higher amounts of stored lipids, which are assumed to be adaptations designed to overcome host resistance and facilitate subsequent oviposition.

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A simple enzymatic microdetermination of cytochrome b5 in erythrocytes

Cited by 2 publications

References 16 publications

Age-dependent decay of cytochrome b5 and cytochrome b5 reductase in human erythrocytes

Age-dependent decay of cytochrome b5 and cytochrome b5 reductase in human erythrocytes

Survival physiology and sex ratio of the Chinese white pine beetleDendroctonus armandi(Coleoptera: Scolytinae) during host colonization and overwintering

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