A Simple In-Vivo Method for Evaluation of Antibiofilm and Wound Healing Activity Using Excision Wound Model in Diabetic Swiss Albino Mice

Alrouji, Mohammed; Kuriri, Fahd A.; Alqasmi, Mohammed; AlSudais, Hamood; Alissa, Mohammed; Alsuwat, Meshari A.; Asad, Mohammed; Joseph, Babu; Almuhanna, Yasir

doi:10.3390/microorganisms11030692

Cited by 6 publications

(5 citation statements)

References 36 publications

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“…There are several animal models for the development of biofilm. The method adopted in this study was developed and validated in our laboratory ( Alrouji et al., 2023 ). The selection of two different concentrations was based on pilot studies and skin irritation studies.…”

Section: Discussionmentioning

confidence: 99%

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Effect of Ducrosia anethifolia methanol extract against methicillin resistant Staphylococcus aureus and Pseudomonas aeruginosa biofilms on excision wound in diabetic mice

Almuhanna

2024

Front. Cell. Infect. Microbiol.

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BackgroundDucrosia anethifolia is an aromatic desert plant used in Saudi folk medicine to treat skin infections. It is widely found in Middle Eastern countries.MethodsA methanolic extract of the plant was prepared, and its phytoconstituents were determined using LC-MS. In-vitro and in-vivo antibacterial and antibiofilm activities of the methanolic extract were evaluated against multidrug-resistant bacteria. The cytotoxic effect was assessed using HaCaT cell lines in-vitro. Diabetic mice were used to study the in-vivo antibiofilm and wound healing activity using the excision wound method.ResultsMore than 50 phytoconstituents were found in the extract after LC-MS analysis. The extract exhibited antibacterial activity against both the tested pathogens. The extract was free of irritant effects on mice skin, and no cytotoxicity was observed on HaCaT cells with an IC50 value of 1381 µg/ml. The ointment formulation of the extract increased the healing of diabetic wounds. The microbial load of both pathogens in the wounded tissue was also reduced after the treatment. The extract was more effective against methicillin-resistant Staphylococcus aureus (MRSA) than MDR-P. aeruginosa in both in vitro and in vivo experiments. Further, skin regeneration was also observed in histological studies.ConclusionsThe results showed that D. anethifolia methanol extract supports wound healing in infected wounds in diabetic mice through antibacterial, antibiofilm, and wound healing activities.

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Section: Discussionmentioning

confidence: 99%

“…This was done using a method standardized in our laboratory ( Alrouji et al., 2023 ). Streptozocin and nicotinamide were used to induce diabetes ( Yan, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

Effect of Ducrosia anethifolia methanol extract against methicillin resistant Staphylococcus aureus and Pseudomonas aeruginosa biofilms on excision wound in diabetic mice

Almuhanna

2024

Front. Cell. Infect. Microbiol.

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“…The study involved both in vitro and in vivo evaluations to confirm its beneficial antibiofilm effect. We selected a mouse model that has been reported earlier as a simple method for the study of the antibiofilm effect [17]. The method involved the use of diabetic mice with an application of preformed biofilm.…”

Section: Discussionmentioning

confidence: 99%

“…For the determination of the antibiofilm activity of the formulation, a method described earlier was used [17]. The pathogens were grown on a coverslip immersed in LB broth in a culture bottle inoculated with either MRSA or P. aeruginosa.…”

Section: Antibiofilm Activity In Vivomentioning

confidence: 99%

Effect of Achillea fragrantissima Extract on Excision Wound Biofilms of MRSA and Pseudomonas aeruginosa in Diabetic Mice

Almuhanna

Alqasmi

AlSudais

et al. 2023

IJMS

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Achillea fragrantissima, a desert plant commonly known as yarrow, is traditionally used as an antimicrobial agent in folklore medicine in Saudi Arabia. The current study was undertaken to determine its antibiofilm activity against methicillin-resistant Staphylococcus aureus (MRSA) and multi-drug-resistant Pseudomonas aeruginosa (MDR-P. aeruginosa) using in vitro and in vivo studies. A biofilm model induced through an excision wound in diabetic mice was used to evaluate its effect in vivo. The skin irritation and cytotoxic effects of the extract were determined using mice and HaCaT cell lines, respectively. The Achillea fragrantissima methanolic extract was analyzed with LC-MS to detect different phytoconstituents, which revealed the presence of 47 different phytoconstituents. The extract inhibited the growth of both tested pathogens in vitro. It also increased the healing of biofilm-formed excision wounds, demonstrating its antibiofilm, antimicrobial, and wound-healing action in vivo. The effect of the extract was concentration-dependent, and its activity was stronger against MRSA than MDR-P. aeruginosa. The extract formulation was devoid of a skin irritation effect in vivo and cytotoxic effect on HaCaT cell lines in vitro.

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“…Mice were positioned dorsal side up on a sterile sheet. A wound of 5 mm in diameter was created (Chen et al, 2021;Alrouji et al, 2023).…”

Section: Animal Modelmentioning

confidence: 99%

Adipose Derived Stem Cell‐Conditioned Media As Alternative of Stem Cell Therapy for Diabetic Wound Healing

Ayesha Nawaz,

Sania Akram,

Hazrat Zaman

et al. 2023

LGUJLS

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Diabetes mellitus (DM) is a set of metabolic disorder primarily characterized by chronic hyperglycaemia state, which is linked with several complications including retinopathy, neuropathy, and delayed wound healing. Adipose derived stem cells conditioned media (ADSCs-CM) contains several cytokines, and growth factors that can be used for treatment of diabetes-associated wounds. This study was conducted to explore the therapeutic potential of ADSCs- CM in excisional wound model in diabetic mice. Diabetes was induced in mice through Streptozotocin (STZ). Then, excisional wound splinting model in diabetic mice was developed. Subcutaneous injections of ADSC-CM (pure) and ADSC-CM conjugated with zinc sulphate were applied on the mice wound bed. Wound diameter, weight, and blood glucose levels of all the mice groups were measured at the start and end of the experiment. Additionally, qPCR was performed to analyse the expression level of some crucial genes (MMP-2, NANOG and OCT4) associated with wound healing. Overall, the results showed reduced wound size, blood glucose level, and weight restoration in ADSCs-CM treated mice as compared with control groups. Furthermore, ADSCs-CM also modulated the gene expression of MMP-2, NANOG, and OCT4, contributing to the enhanced wound healing observed in diabetic mice. This suggests ADSC-CM’s potential as an alternative therapeutic option for achieving improved wound healing outcomes in diabetic patients.

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A Simple In-Vivo Method for Evaluation of Antibiofilm and Wound Healing Activity Using Excision Wound Model in Diabetic Swiss Albino Mice

Cited by 6 publications

References 36 publications

Effect of Ducrosia anethifolia methanol extract against methicillin resistant Staphylococcus aureus and Pseudomonas aeruginosa biofilms on excision wound in diabetic mice

Effect of Ducrosia anethifolia methanol extract against methicillin resistant Staphylococcus aureus and Pseudomonas aeruginosa biofilms on excision wound in diabetic mice

Effect of Achillea fragrantissima Extract on Excision Wound Biofilms of MRSA and Pseudomonas aeruginosa in Diabetic Mice

Adipose Derived Stem Cell‐Conditioned Media As Alternative of Stem Cell Therapy for Diabetic Wound Healing

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