A simple lectin-based biochip might display the potential clinical value of glycomics in patients with spontaneous intracerebral hemorrhage

Abstract

Background: Intracerebral hemorrhage (ICH) is a cerebrovascular disease with extremely high disability and mortality rates. Glycans play critical roles in biological processes. However, whether glycans can serve as potential biomarkers for determining clinical diagnosis and prognosis in ICH remains determined. Methods: In this study, we established a lectin-biochip to measure serum glycans levels in ICH patients (n=48) and healthy controls (n=16). An enzyme-linked immunosorbent assay (ELISA) was carried out to determine serum levels of IL-10 and TNF-α in the patients. Correlation analyses of the serum glycan and cytokine levels and the clinicopathological parameters of patients were performed. Results: The biochip-based data revealed that the serum levels of α-Man/α-Glc (ConA), Galβ3GalNAc (PNA), GalNAc (VVA), Fucα6GlcNAc (AAL), α-Fuc (LTL), and Galβ3GalNAc-Ser/Thr (AIL) significantly increased in the super-acute phase of ICH in comparison with healthy controls. Clinicopathological analysis indicated the serum levels of ConA, VVA, and LTL had significant associations with the National Institute of Health Stroke Scale (NIHSS), and serum VVA levels had a significant association with the Mini-Mental State Examination (MMSE) at day 90 after ICH. Correlation coefficient analysis revealed significant correlations between TNF-α and ConA (P<0.001) as well as between IL-10 and ConA (P<0.001), PNA (P=0.02), VVA (P<0.001), and MAL (P=0.04), respectively.Conclusions: We established a proof-of-concept platform for detecting serum glycomics and highlighted their potential value in diagnosing and predicting ICH patients' outcomes.

T‐antigen as a biomarker of progression‐free survival in patients with glioblastoma

“…We described these lectins, which specifically bind to different glycans, in our previous study. 17 Each lectin was dissolved in 10 mM HEPES buffer (pH 8.5) with 0.001% BSA to a concentration of 1 mg/mL. The lectin solutions were individually spotted onto the biochip surface and incubated at 25°C for 2 h to form a lectin probe‐coated surface.…”

“…Lectins, including Lotus tetragonolobus lectin (LTL, L‐1320), Lens culinaris agglutinin (LCA, L‐1040), Narcissus pseudonarcissus lectin (NPL, L‐1370), Jacalin (L‐1150), Ricinus communis agglutinin I (RCA‐I, L‐1080), Peanut agglutinin (PNA, L‐1070), Vicia villosa lectin (VVL, L‐1230), Wheat germ agglutinin (WGA, L‐1020), Maackia amurensis lectin I (MAL‐I, L‐1310), Sambucus nigra lectin (SNA, L‐1300), and concanavalin A (Con A L‐1000‐500), were commercially purchased from Vector Laboratories (CA, USA). We described these lectins, which specifically bind to different glycans, in our previous study 17 . Each lectin was dissolved in 10 mM HEPES buffer (pH 8.5) with 0.001% BSA to a concentration of 1 mg/mL.…”

Clinical Value of Glycan Changes in Cerebrospinal Fluid for Evaluation of Post-Neurosurgical Bacterial Meningitis with Hemorrhagic Stroke Patients

“…This study recruited 136 patients with hemorrhagic stroke of whom 62 were diagnosed with intracerebral hemorrhage (ICH) and 74 were diagnosed with aneurysmal subarachnoid hemorrhage (aSAH). PNBM was diagnosed in 53 out of the 136 patients based on the diagnostic criteria issued by IDSA's Clinical Practice Guidelines for Healthcare-Associated Ventriculitis and Meningitis 2017 [23] and a Chinese Expert Consensus of Diagnostic and Therapy for the Neurosurgical Central Nervous System Infections in 2021. Demographic and clinicopathological features are summarized in Table 1.…”