A Simple, Low-Cost Platform for Real-Time Isothermal Nucleic Acid Amplification

Craw, Pascal; Mackay, Ruth; Naveenathayalan, Angel; Hudson, Christopher R.; Branavan, Manoharanehru; Sadiq, S Tariq; Balachandran, W.

doi:10.3390/s150923418

Cited by 20 publications

(17 citation statements)

References 15 publications

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“…Although not all for ZIKV, several groups have already developed low-cost devices using inexpensive insulating materials (e.g. thermoses) [40, 43, 44] and simple heat-producing elements [45–48], including non-electrical exothermal reactions [43, 49, 50] (Fig. 4b,c).…”

Section: Developmental Diagnostic Methodsmentioning

confidence: 99%

Future developments in biosensors for field-ready Zika virus diagnostics

2017

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Since early reports of the recent Zika virus outbreak in May 2015, much has been learned and discussed regarding Zika virus infection and transmission. However, many opportunities still remain for translating these findings into field-ready sensors and diagnostics. In this brief review, we discuss current diagnostic methods, consider the prospects of translating other flavivirus biosensors directly to Zika virus sensing, and look toward the future developments needed for high-sensitivity and high-specificity biosensors to come.

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Section: Developmental Diagnostic Methodsmentioning

confidence: 99%

Future developments in biosensors for field-ready Zika virus diagnostics

2017

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“…The results of this study could be used to develop fast and cost-efficient tests (Craw et al, 2015 ) to detect the presence of specific pathogens or their virulence factors in enclosed environments such as public transport, pharmaceutical cleanrooms, hospitals, and intensive care units. This study has broadened our understanding of the role of pathogens in such highly controlled environments and should be considered for microbial monitoring of the ISS during sustained presence of humans in space and future manned missions to Mars.…”

Section: Discussionmentioning

confidence: 99%

Functional Metagenomics of Spacecraft Assembly Cleanrooms: Presence of Virulence Factors Associated with Human Pathogens

et al. 2016

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Strict planetary protection practices are implemented during spacecraft assembly to prevent inadvertent transfer of earth microorganisms to other planetary bodies. Therefore, spacecraft are assembled in cleanrooms, which undergo strict cleaning and decontamination procedures to reduce total microbial bioburden. We wanted to evaluate if these practices selectively favor survival and growth of hardy microorganisms, such as pathogens. Three geographically distinct cleanrooms were sampled during the assembly of three NASA spacecraft: The Lockheed Martin Aeronautics' Multiple Testing Facility during DAWN, the Kennedy Space Center's Payload Hazardous Servicing Facility (KSC-PHSF) during Phoenix, and the Jet Propulsion Laboratory's Spacecraft Assembly Facility during Mars Science Laboratory. Sample sets were collected from the KSC-PHSF cleanroom at three time points: before arrival of the Phoenix spacecraft, during the assembly and testing of the Phoenix spacecraft, and after removal of the spacecraft from the KSC-PHSF facility. All samples were subjected to metagenomic shotgun sequencing on an Illumina HiSeq 2500 platform. Strict decontamination procedures had a greater impact on microbial communities than sampling location Samples collected during spacecraft assembly were dominated by Acinetobacter spp. We found pathogens and potential virulence factors, which determine pathogenicity in all the samples tested during this study. Though the relative abundance of pathogens was lowest during the Phoenix assembly, potential virulence factors were higher during assembly compared to before and after assembly, indicating a survival advantage. Decreased phylogenetic and pathogenic diversity indicates that decontamination and preventative measures were effective against the majority of microorganisms and well implemented, however, pathogen abundance still increased over time. Four potential pathogens, Acinetobacter baumannii, Acinetobacter lwoffii, Escherichia coli and Legionella pneumophila, and their corresponding virulence factors were present in all cleanroom samples. This is the first functional metagenomics study describing presence of pathogens and their corresponding virulence factors in cleanroom environments. The results of this study should be considered for microbial monitoring of enclosed environments such as schools, homes, hospitals and more isolated habitation such the International Space Station and future manned missions to Mars.

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“…There are various reports of device innovations to improve the device portability and cost. Researchers have miniaturized optical detection methods from microscopy [ 112 , 198 , 199 ] and integrated with novel light sources like organic LEDs, filter-free photodiodes [ 200 ], and waveguides [ 201 ] to enhance the signal intensity and assay sensitivity, primarily in PCR-based systems. Hung et al [ 202 ] developed supercritical angle fluorescence (SAF) detection with a microlens array for use with solid-phase PCR.…”

Section: Detectionmentioning

confidence: 99%

Towards Multiplex Molecular Diagnosis—A Review of Microfluidic Genomics Technologies

Basha

Yousuff

et al. 2017

Micromachines

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Highly sensitive and specific pathogen diagnosis is essential for correct and timely treatment of infectious diseases, especially virulent strains, in people. Point-of-care pathogen diagnosis can be a tremendous help in managing disease outbreaks as well as in routine healthcare settings. Infectious pathogens can be identified with high specificity using molecular methods. A plethora of microfluidic innovations in recent years have now made it increasingly feasible to develop portable, robust, accurate, and sensitive genomic diagnostic devices for deployment at the point of care. However, improving processing time, multiplexed detection, sensitivity and limit of detection, specificity, and ease of deployment in resource-limited settings are ongoing challenges. This review outlines recent techniques in microfluidic genomic diagnosis and devices with a focus on integrating them into a lab on a chip that will lead towards the development of multiplexed point-of-care devices of high sensitivity and specificity.

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A Simple, Low-Cost Platform for Real-Time Isothermal Nucleic Acid Amplification

Cited by 20 publications

References 15 publications

Future developments in biosensors for field-ready Zika virus diagnostics

Future developments in biosensors for field-ready Zika virus diagnostics

Functional Metagenomics of Spacecraft Assembly Cleanrooms: Presence of Virulence Factors Associated with Human Pathogens

Towards Multiplex Molecular Diagnosis—A Review of Microfluidic Genomics Technologies

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