A simple medium enables bovine embryos to be held for seven days at 4°C

Ideta, Atsushi; Aoyagi, Yoshito; Tsuchiya, K.; Kamijima, Tatsuro; Nishimiya, Yoshiyuki; Tsuda, Sakae

doi:10.1038/srep01173

Cited by 30 publications

(36 citation statements)

References 38 publications

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“…In both experiments, blastocysts produced from oocytes matured in a medium containing 800 pg/ml of ghrelin appeared to be of better quality in comparison with their counterparts, as the hatching rate – used as a quality marker (Ideta et al. ) – was significantly increased. Serum was present in our embryo production system.…”

Section: Discussionmentioning

confidence: 90%

Ghrelin Accelerates In Vitro Maturation of Bovine Oocytes

Dovolou

Messinis

Periquesta

et al. 2014

Reprod Domestic Animals

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Ghrelin, apart from its metabolic role, is nowadays considered as a basic regulator of reproductive functions of mammals, acting at central and gonadal levels. Here, we investigated for possible direct actions of ghrelin on in vitro maturation of bovine oocytes and for its effects on blastocyst yield and quality. In experiment 1, cumulus oocyte complexes (COCs) were matured in the presence of four different concentrations of ghrelin (0, 200, 800 and 2000 pg/ml). In vitro fertilization and embryo culture were carried out in the absence of ghrelin, and blastocyst formation rates were examined on days 7, 8 and 9. In experiment 2, only the 800 pg/ml dose of ghrelin was used. Four groups of COCs were matured for 18 or 24 h (C18, Ghr18, C24 and Ghr24), and subsequently, they were examined for oocyte nuclear maturation and cumulus layer expansion; blastocysts were produced as in experiment 1. The relative mRNA abundance of various genes related to metabolism, oxidation, developmental competence and apoptosis was examined in snap-frozen cumulus cells, oocytes and day-7 blastocysts. In experiment 1, ghrelin significantly suppressed blastocyst formation rates. In experiment 2, more ghrelin-treated oocytes matured for 18 h reached MII compared with controls, while no difference was observed when maturation lasted for 24 h. At 18 and 24 h, the cumulus layer was more expanded in ghrelin-treated COCs than in the controls. The blastocyst formation rate was higher in Ghr18 (27.7 ± 2.4%) compared with Ghr24 (17.5 ± 2.4%). Differences were detected in various genes' expression, indicating that in the presence of ghrelin, incubation of COCs for 24 h caused over-maturation (induced ageing) of oocytes, but formed blastocysts had a higher hatching rate compared with the controls. We infer that ghrelin exerts a specific and direct role on the oocyte, accelerating its maturational process.

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Section: Discussionmentioning

confidence: 90%

Ghrelin Accelerates In Vitro Maturation of Bovine Oocytes

Dovolou

Messinis

Periquesta

et al. 2014

Reprod Domestic Animals

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“…Ideta et al () reported that the addition of serum with a high concentration in the storage medium enhances viability of bovine embryos after hypothermic preservation compared with BSA addition. They suggested that bovine embryos could be stored in 100% bovine serum at hypothermic temperatures (Ideta et al, ). Serum provides energy substrates and amino acids for metabolic and anabolic processes as well as chelation of heavy metal ions or other toxins (Shah, ).…”

Section: Introductionmentioning

confidence: 99%

Hypothermic storage of porcine zygotes in serum supplemented with chlorogenic acid

Nguyen

Hirata

Tanihara

et al. 2019

Reprod Domestic Animals

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Contents The current study was conducted to investigate the effects of 100% foetal bovine serum (FBS) and 100% porcine follicular fluid (pFF) as a storage medium on the developmental competence of porcine zygotes stored at 25°C for 24 hr. Moreover, we evaluated the additive effects of chlorogenic acid (CGA) in the storage medium. When in vitro‐produced zygotes were stored at 25°C for 24 hr in tubes containing either tissue culture medium (TCM) 199 supplemented with 1 mg/ml bovine serum albumin (BSA), 100% of FBS or 100% of pFF, the rate of blastocyst formation was significantly higher in 100% of FBS than in BSA‐containing TCM 199. When the effects of CGA supplementation in 100% of FBS on the development of zygotes stored at 25°C for 24 hr was evaluated, more zygotes stored with 50 µM CGA developed to blastocysts compared with the other concentrations of CGA. When the formation date and quality of blastocysts derived from zygotes stored in 100% of FBS supplemented with 50 µM CGA were investigated, the highest ratio of blastocysts formation in the storage group appeared 1 day later than in the non‐stored control group. However, a higher proportion of blastocysts with apoptotic nuclei was observed in the stored group as compared to the non‐stored group. In conclusion, 100% of FBS is available for a short storage medium of porcine zygotes. The supplementation of 50 µM CGA into the storage medium improves the rates of blastocyst formation of zygotes after storage, but the quality of embryos from the stored zygotes remains to be improved.

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“…Similarly, serum supplementation during the hypothermic storage of bovine embryos was reported to maintain their viability (Ideta et al . ). Furthermore, supplementing a basic holding medium with serum was reported to maintain the viability of immature porcine oocytes preserved under ambient temperatures (Yang et al .…”

Section: Discussionmentioning

confidence: 97%

“…Under hypothermic conditions serum also seems to act positively on the viability of bovine embryos (Ideta et al . ). Antioxidants such as α‐tocopherol, cysteamine, cysteine (Wang et al .…”

Section: Introductionmentioning

confidence: 97%

“…Increasing pyruvate concentration and neglecting glucose in medium combined with hypothermic conditions have been reported to prevent aging in mouse oocytes by the downregulation of mitochondrial metabolism (Li et al 2012;Wang et al 2014). Under hypothermic conditions serum also seems to act positively on the viability of bovine embryos (Ideta et al 2013). Antioxidants such as α-tocopherol, cysteamine, cysteine (Wang et al 2014), melatonin (Lord et al 2013) or dithiotreitol (DTT) (Tarin et al 1998;Rausell et al 2007) have been reported to prevent/moderate the detrimental effects of ROS in aged mouse oocytes.…”

Section: Introductionmentioning

confidence: 99%

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Effect of medium additives during liquid storage on developmental competence of in vitro matured bovine oocytes

Suttirojpattana

Somfai

Matoba

et al. 2016

Animal Science Journal

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Our aim was to improve the developmental competence of bovine oocytes during their liquid storage by using additives. In vitro matured oocytes were stored for 20 h at 25°C in HEPES buffered TCM 199 medium (base medium). After storage, in vitro embryo development after in vitro fertilization was compared to those of non‐stored (control) ones. Addition of 10% (v/v) newborn calf serum or 10.27 mmol/L pyruvate alone to the base medium did not improve blastocyst formation rates in stored oocytes; however, their simultaneous addition significantly improved the rate compared with those stored in base medium (P < 0.05). Supplementation of the holding medium with dithiothreitol (DTT) at any concentrations did not improve embryo development from stored oocytes. Although supplementation with cyclosporine A (CsA) significantly reduced apoptosis and membrane damage rates during storage, it did not improve the developmental competence of oocytes. 1,2‐bis(2‐aminophenoxy) ethane N,N,N’,N’‐tetraacetic acid tetrakis‐acetoxymethyl ester and ruthenium red had no effect on oocyte apoptotic rates. Blastocyst formation rates in all stored groups remained significantly lower than that of the control. In conclusion, pyruvate and serum had a synergic effect to moderate the reduction of oocyte quality during storage, whereas mitochondrial membrane pore inhibitor CsA and the antioxidant DTT did not affect their developmental competence.

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A simple medium enables bovine embryos to be held for seven days at 4°C

Cited by 30 publications

References 38 publications

Ghrelin Accelerates In Vitro Maturation of Bovine Oocytes

Ghrelin Accelerates In Vitro Maturation of Bovine Oocytes

Hypothermic storage of porcine zygotes in serum supplemented with chlorogenic acid

Effect of medium additives during liquid storage on developmental competence of in vitro matured bovine oocytes

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