A simple method of storing nonsynnematous and synnematous isolates of Hirsutella thompsonii while conserving their pleomorphism, pathogenicity and genetic purity

Abstract

Five nonsynnematous and six synnematous isolates of the acaropathogenic fungus Hirsutella thompsonii were successfully stored as mycelial beads in sterile water under refrigeration. They were viable for up to 96 months without any adverse effect on their pleomorphic characteristics. Besides, there was loss neither in pathogenicity nor in genetic purity. Thus, mycelial beads can now be an addition to the list of fungal propagules that are amenable to storage in sterile water over a long period.