A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels

Identification, cloning, sequencing, and overexpression of the gene encoding proclavaminate amidino hydrolase and characterization of protein function in clavulanic acid biosynthesis

“…The temperatures and time periods used in each cycle were 94ЊC for 1 min, 58ЊC for 1 min, and 72ЊC for 1 min. The amplified DNA fragment (0.96 kb) was isolated from a low-melting-point agarose gel (46), digested with endonucleases EcoRI and HindIII, and ligated into the pMAL-c2 vector at the EcoRI-HindIII cleavage sites. To check for errors owing to base misincorporation during PCR (8), the amplified fragment was independently sequenced.…”

Nucleotide sequence of satellite DNA contained in the eliminated genome ofAscaris lumbricoides

“…The 120 bp satellite monomer was localized by staining with ethidium bromide and the corresponding part of the gel was cut out. Isolation of the DNA from the gel was performed following the modified method of Wieslander (19). After addition of V10 of the volume of 100 mM Tris (HC1), pH 7.8, 5 M NaCl, 10 mM EDTA, the gel slices were melted at 680 C for 10 minutes followed by an incubation at 370 C for another 10 minutes.…”

Structural Characteristics of Nairoviruses (Genus Nairovirus, Bunyaviridae)

“…Viral RNA analyses. The procedures used for extraction of viral RNA from pelleted virus, the resolution of RNA species by electrophoresis in gels of polyacrylamide (Roy & Bishop, 1972) or agarose (Wieslander, 1979), and identification and recovery of the viral RNA species from agarose gels, have been described previously. The gels were fractionated with a Mickle gel slicer (Brinkmann, Gomshall, Surrey, U.K.).…”