A simple on-line fibre optics UV detector for microcolumn HPLC

Janeček, M.; Foret, František; Šlais, Karel; Boček, Petr

doi:10.1007/bf02262091

Cited by 9 publications

(6 citation statements)

References 5 publications

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“…Despite the extremely small path lengths, detection is feasible at the sub-ng level. Fiber optics have been suggested to collimate the excitation light onto the flow cell and for the collection of the UV light that has passed through the flow cell [139,140]. The limits of detection that were obtained with this system are, however, not favorable compared to on-column detection.…”

Section: Uv Absorbance Detectionmentioning

confidence: 99%

Microcolumn liquid chromatography: instrumentation, detection and applications

Vissers¹,

Claessens

Cramers

1997

Journal of Chromatography A

208

101

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This review discusses many different aspects of microcolumn liquid chromatography (LC) and reflects the areas of microcolumn LC research interest over the past decades. A brief theoretical discussion on a number of major issues, such as column characterisation, chromatographic dilution effects and extracolumn band broadening in microcolumn LC is given. Recent progress in column technology and the demands and developments of instrumentation and accessories for microcolumn LC are also reviewed. Besides that, the developments in a large number of established and also more recent detection techniques for microcolumn LC are also discussed. The potential of hyphenation of microcolumn LC with other techniques, more particularly of multidimensional chromatography and microcolumn LC coupled to mass spectrometry is reviewed. Finally, the perspective of microcolumn LC separation methods is stressed by a number of relevant applications.

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Section: Uv Absorbance Detectionmentioning

confidence: 99%

Microcolumn liquid chromatography: instrumentation, detection and applications

Vissers¹,

Claessens

Cramers

1997

Journal of Chromatography A

208

101

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“…and 0.22 mm 0.d. (9). This capillary is inserted into the capillary (1) to contact the adjoining capillary (16).…”

Section: Microcolumn Liquid Chromatographmentioning

confidence: 99%

“…A detection cell is placed at the end of the column. The miniaturized cell of the UV detector, with optical fibers [8,9], in which the volume does not exceed 30 nL exhibits parameters suitable for application in the LC/GC combination. The cell is connected by the capillary through an interface with the gas chromatograph.…”

Section: Microcolumn Liquid Chromatographmentioning

confidence: 99%

“…The cell is connected by the capillary through an interface with the gas chromatograph. The ends of the capillaries are adjusted so that they can be easily connected together (8), as well as connected to the UV detector of the liquid chromatograph (9) and to the gap column of the gas capillary (2), exceeding the extended part of the capillary approximately by 15 mm and reaching the gap. At the other end, it passes through a seal (15) and enters by a distance of 1 to 2 mm into the capillary (1).…”

Section: Microcolumn Liquid Chromatographmentioning

confidence: 99%

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New μLC/GC interface

Rusek

Ciganková

Krejčí

et al. 1994

J Microcolumn Separations

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“…Optical fibers have also been useful in thermal lens spectrometry (14)(15)(16), as an optic providing well-defined beam properties. Optical fiber technology has also been incorporated into LC and flow injection analysis (FIA) absorbance detection methods (10,14,(17)(18)(19)(20). Although open optics are also popular, fiber optics solve detection problems associated with miniaturization.…”

Section: Introductionmentioning

confidence: 99%