A Simple Paper-Based α-Amylase Separating System for Potential Application in Biological Sciences

Hyung, Sujin; Karima, Gul; Shin, Kyusoon; Kim, Kwang Sik; Hong, Jong Wook

doi:10.1007/s13206-021-00022-3

Cited by 5 publications

(5 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The advantage of this method is that paper-based sensors are less expensive to manufacture, but the distribution of α-amylase during the infiltration of liquid specimens into the paper needs to be considered. Hyung et al conducted a study on this and found that α-amylase aggregates at the head of the paper [66]. Applying starch only to the head of the paper, they found that there was also no α-amylase distribution at the end where there was no starch, indicating that the amylase had been depleted previously.…”

Section: Amylose-iodine Colorimetrymentioning

confidence: 99%

Sensing of Digestive Enzymes—Diagnosis and Monitoring of Pancreatitis

Yin,

Cui,

Yang

et al. 2023

Chemosensors

View full text Add to dashboard Cite

This paper is a comprehensive review of the techniques for the detection of pancreatic enzymes, which are common biochemical indicators of pancreatitis, including amylase, trypsin, chymotrypsin, elastase, and lipase. Pancreatitis is a disease with self-digestion due to the abnormal activation of digestive enzymes in the pancreas. Hospitalization is often required due to the lack of convenient therapeutic agents. The main recent results are reported in this review, especially the techniques that enable portability and Point-of-Care testing (POCT). This is because timely diagnosis at the early stage and avoiding recurrence after recovery are the keys to treatment. It is also important to reduce the rate of misdiagnosis and to avoid overtreatment. Various detection methods are discussed, with particular attention given to the implementation of chemical sensing and probe design. The new sensing technology for digestive enzymes makes it possible to perform early screening for pancreatitis in remote areas or in one’s own home.

show abstract

Section: Amylose-iodine Colorimetrymentioning

confidence: 99%

Sensing of Digestive Enzymes—Diagnosis and Monitoring of Pancreatitis

Yin,

Cui,

Yang

et al. 2023

Chemosensors

View full text Add to dashboard Cite

show abstract

“…It exists in two proteoforms: non-glycosylated (∼56 kDa) and glycosylated (∼59 kDa) [31] , [32] , [33] . Various techniques are employed for α-amylase depletion, including the use of a syringe-based potato starch device [34] , gel filtration [35] , affinity chromatography with lectin ConA [31] , co-precipitation and paper-based chips [36] . Deutsch et al .…”

Section: Introductionmentioning

confidence: 99%

Enhancing saliva diagnostics: The impact of amylase depletion on MALDI-ToF MS profiles as applied to COVID-19

LaCasse,

Chivte,

Kress

et al. 2024

Journal of Mass Spectrometry and Advances in the Clinical Lab

View full text Add to dashboard Cite

“…It exists in two proteoforms: non-glycosylated (~56 kDa) and glycosylated (~59 kDa). [31], [32], [33] Several techniques are currently utilized for α-amylase depletion including a syringe-based potato starch device [34], gel filtration [35], affinity chromatography by lectin ConA [31], co-precipitation and paper-based chips [36]. Deutsch et al designed and patented an amylase depleting device to separate salivary α-amylase from the whole saliva by affinity adsorption to potato starch.…”

Section: Introductionmentioning

confidence: 99%

Amylase Depletion Significantly Improves the Maldi-Tof Profile of Saliva – Application to Covid-19 Diagnostics

LaCasse

Chivte

Kress

et al. 2023

Preprint

View full text Add to dashboard Cite

Human saliva contains a plethora of proteins whose presence and concentration can be monitored for diagnosis and progression of disease. Saliva has been extensively probed for the diagnosis of several systemic and infectious diseases because of the ease with which it can be collected. However, amylase, the most abundant protein found in saliva can obscure the detection of low-abundance proteins by MALDI-ToF MS (matrix-assisted laser desorption/ionization-time of flight mass spectrometry) and diminish the diagnostic utility of this specimen type. In the present study, we used a device to deplete salivary amylase from water-gargle samples through affinity adsorption. After depletion, profiling of the saliva proteome was performed by MALDI-ToF MS on gargle samples from subjects whose COVID-19 (coronavirus disease 2019) status was confirmed by NP (nasopharyngeal) swab RT-qPCR (reverse transcription polymerase chain reaction). Amylase depletion led to the enhancement of signal intensities of various peaks as well as the detection of previously unobserved peaks in the MALDI-ToF spectra. The overall specificity and sensitivity after amylase depletion was 100% and 85.17% respectively for detecting COVID-19. Our simple, rapid and inexpensive technique to deplete salivary amylase can be used to unmask spectral diversity in saliva by MALDI-ToF MS, reveal low-abundant proteins and aid in the establishment of novel biomarkers for diseases.

show abstract

A Simple Paper-Based α-Amylase Separating System for Potential Application in Biological Sciences

Cited by 5 publications

References 34 publications

Sensing of Digestive Enzymes—Diagnosis and Monitoring of Pancreatitis

Sensing of Digestive Enzymes—Diagnosis and Monitoring of Pancreatitis

Enhancing saliva diagnostics: The impact of amylase depletion on MALDI-ToF MS profiles as applied to COVID-19

Amylase Depletion Significantly Improves the Maldi-Tof Profile of Saliva – Application to Covid-19 Diagnostics

Contact Info

Product

Resources

About