A simple, rapid and yet less expensive method to detect chitinase in agarose plates

Anil, Kondreddy; Seshagirirao, Kottapalli; Podile, Appa Rao

doi:10.1016/j.jbbm.2007.01.015

Cited by 10 publications

(8 citation statements)

References 6 publications

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“…After solidification, 20 mL of sterile distilled water, 10 mg of chitinase from Streptomyces griceus (Sigma-Aldrich), and 10 mg of recombinant CTL1 were pipetted into separate wells in the plate. Plates were incubated at 37°C for 2 h, stained with 0.01% of Calcofluor White M2R (Sigma-Aldrich), washed with distilled water, and visualized under UV light to detected the zone of clearance around the well left by the degradation of chitin by chitinase activity (Anil et al, 2007).…”

Section: Chitinase Activity Assaymentioning

confidence: 99%

Chitinase-Like Protein CTL1 Plays a Role in Altering Root System Architecture in Response to Multiple Environmental Conditions

et al. 2009

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Plant root architecture is highly responsive to changes in nutrient availability. However, the molecular mechanisms governing the adaptability of root systems to changing environmental conditions is poorly understood. A screen for abnormal root architecture responses to high nitrate in the growth medium was carried out for a population of ethyl methanesulfonatemutagenized Arabidopsis (Arabidopsis thaliana). The growth and root architecture of the arm (for anion altered root morphology) mutant described here was similar to wild-type plants when grown on low to moderate nitrate concentrations, but on high nitrate, arm exhibited reduced primary root elongation, radial swelling, increased numbers of lateral roots, and increased root hair density when compared to the wild-type control. High concentrations of chloride and sucrose induced the same phenotype. In contrast, hypocotyl elongation in the dark was decreased independently of nitrate availability. Positional cloning identified a point mutation in the AtCTL1 gene that encodes a chitinase-related protein, although molecular and biochemical analysis showed that this protein does not possess chitinase enzymatic activity. CTL1 appears to play two roles in plant growth and development based on the constitutive effect of the arm mutation on primary root growth and its conditional impact on root architecture. We hypothesize that CTL1 plays a role in determining cell wall rigidity and that the activity is differentially regulated by pathways that are triggered by environmental conditions. Moreover, we show that mutants of some subunits of the cellulose synthase complex phenocopy the conditional effect on root architecture under nonpermissive conditions, suggesting they are also differentially regulated in response to a changing environment.

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Section: Chitinase Activity Assaymentioning

confidence: 99%

Chitinase-Like Protein CTL1 Plays a Role in Altering Root System Architecture in Response to Multiple Environmental Conditions

et al. 2009

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“…MMC1 was more efficient for recovering chitinolytic bacteria from the environmental samples. Bacteria associated with plankton and particulates occur at higher concentrations than as freeliving oceanic bacteria, whether freshwater, coastal, or estuarine systems (Bidle and Flecher 1995), which explains Skujins et al (1965) b Roberts and Selitrennikoff (1988) modified by Wen et al (2002) c Wet weight -Not used (Ramírez et al 2004;Anil et al 2007), which increases the cost of the experiment. However, without the addition of these substrates, the halo produced by chitinolytic bacteria obtained in this study was seen easily.…”

Section: Resultsmentioning

confidence: 99%

Culture medium for isolating chitinolytic bacteria from seawater and plankton

Souza

Burbano-Rosero

Almeida

et al. 2009

World J Microbiol Biotechnol

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Chitin degradation is a key step in the cycling of nutrients in marine ecosystems and chitinolytic bacteria are the primary agents of this process. Chitinases, produced by bacteria, have potential applications in agriculture, medicine and in a wide range of biotechnological processes. We utilized a simple, rapid and cost-effective method of colloidal chitin preparation and a culture medium, in which colloidal chitin is the sole carbon source for the purpose of counting and isolating chitinolytic bacteria from seawater and plankton. This culture medium could be useful to isolate bacteria with the ability to produce extracellular chitinases for biotechnological applications.

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“…The insect cuticle is composed of chitin. Chitin is a polymer of N-acetyl glucosamine and the second abundant component on the Contact time (h) X 2 1 72 37 surface of the earth after cellulose [9]. It is present in bacterial, yeasts and fungi cell walls [10].…”

Section: Methodsmentioning

confidence: 99%

Effectiveness of a Biological Insecticide Derived from Scorpion Fish Offal against the Chickpea Weevil

Laribi-Habchi¹

2014

J Bioprocess Biotech

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The aim of this study is to evaluate the biological activity of a crude chitinase extracted from fish offal (scorpion) on the chickpea weevil Callosobruchus maculatus (Coleoptera: Bruchidae) at various concentrations: 3%, 6%, 9%, 12%, 15%, 18%, 21% and 24% (V/V). The biological tests were performed under the typical experimental conditions: temperature and relative humidity at 28°C and 75%, respectively. The results have shown that the concentration of 24% of crude chitinase extracted has 100% of insecticide activity after a contact time of 1 h. The lethal dose (LD50) is estimated for every sample and for a range time from 1 to 72 h. The lower percentage of mortality (10%) is obtained for low values of concentrations and contact time (dose of 3% of crude chitinase and 1 h of contact time). The maximum mortality rate is observed for the high values of two parameters (dose of 24% of crude chitinase and 72 h contact time). However, it is observed that for a higher dose (24%) and for a lower contact time (1 h), the mortality rate increased rapidly. The novelty of this work consists in the use of a new bioinsecticide obtained from offal scorpion fish.

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A simple, rapid and yet less expensive method to detect chitinase in agarose plates

Cited by 10 publications

References 6 publications

Chitinase-Like Protein CTL1 Plays a Role in Altering Root System Architecture in Response to Multiple Environmental Conditions

Chitinase-Like Protein CTL1 Plays a Role in Altering Root System Architecture in Response to Multiple Environmental Conditions

Culture medium for isolating chitinolytic bacteria from seawater and plankton

Effectiveness of a Biological Insecticide Derived from Scorpion Fish Offal against the Chickpea Weevil

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