A Simple, Rapid, Fluorometric Assay for Dopamine by In Situ Reaction of Boronic Acids and cis-Diol

Abstract

An efficient, sensitive, and low-cost method has been developed for turn-on fluorescence sensing of dopamine (DA). The method relies on the rapid reaction of DA and 3-Hydroxyphenylboronic acid (3-HPBA) via specific recognition between boronic acids and cis-diol of DA in alkaline solution. The reaction product shows an excitation wavelength of 417 nm and the maximum emission peak at 470 nm. The proposed method allows the determination of DA in the range of 50 nM–25 μM, and the whole detection can be completed within 5 minutes. Furthermore, the presented approach has good selectivity and has been successfully applied to DA sensing in human serum samples, showing great potential in clinical diagnosis.

Rapid exocytosis kinetics measured by amperometry within volcano microelectrodes

“…The catecholamine content of PC12 cells was determined by fluorometric measurement following reaction of catecholamines with 3-hydroxyphenyl boronic acid. 60 The detailed lysis and assay protocol are detailed in the ESI (section 4 and ESI Fig. 6 † ).…”

Coumarin‐Pyrazole Based Fluorescent Sensor for Selective Detection of Dopamine in Aqueous Medium

“…The literature contains various examples of dopamine detection, [18] including dopamine transport agents and other receptors that work only in the organic medium [19] . Aqueous detection of dopamine is accomplished using membrane‐associate receptors, sol gel films and solid state sensors [20] .…”

“…The literature contains various examples of dopamine detection, [18] including dopamine transport agents and other receptors that work only in the organic medium. [19] Aqueous detection of dopamine is accomplished using membraneassociate receptors, sol gel films and solid state sensors. [20] Cyclophanes have been used for selective detection of dopamine that also includes the recent example of shape selective recognition with non-covalent interactions.…”

Simultaneous Electrochemical Detection of Ascorbic Acid, Dopamine, and Uric Acid at Magnetic Nanoparticles/Reduced Graphene Oxide Modified Electrode

“…In recent past, a few analytical techniques have been developed for the simultaneous determination of AA, DA, and UA which includes chromatography [7], fluorimetry [8], chemiluminescence [9], and capillary electrophoresis mass spectrometry method [10]. Although these approaches offer excellent sensitivity in AA, DA, and UA sensing, they often have limitations, including a complex system, a lengthy analysis time, high analyte consumption, hence the need for improvement over time.…”