A simple spectrophotometric method for metallothionein evaluation in marine organisms: an application to Mediterranean and Antarctic molluscs

Viarengo, Aldo; Ponzano, Enrica; Dondero, Francesco; Fabbri, Rita

doi:10.1016/s0141-1136(96)00103-1

Cited by 801 publications

(329 citation statements)

References 30 publications

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“…After acidic ethanol/chloroform fractionation of the tissue homogenate, the levels of MT protein were evaluated using Ellman's reagent by the spectrophotometric assay as described in Viarengo et al (1997). The amount of MT protein was calculated based on a GSH standard curve and presented as nmol of SH groups per mg of protein (nmol/mg protein).…”

Section: Biochemical Assaysmentioning

confidence: 99%

Multi-biomarker responses as indication of contaminant effects in Gambusia affinis from impacted rivers by municipal effluents

Huang

Liu

Liang

et al. 2016

Science of The Total Environment

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Section: Biochemical Assaysmentioning

confidence: 99%

Multi-biomarker responses as indication of contaminant effects in Gambusia affinis from impacted rivers by municipal effluents

Huang

Liu

Liang

et al. 2016

Science of The Total Environment

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“…MTs concentration was measured in digestive glands (molluscs) and liver (fish) tissues according to Viarengo et al (1997), on a Perkin Elmer UV/VIS spectrophotometer Lamda 20. The method is based on the estimation of the sulphydryl content of MTs proteins by spectrophotometric determination of the -SH groups using Ellman's reagent.…”

Section: Metallothioneins Contentmentioning

confidence: 99%

Biochemical biomarker responses to pollution in selected sentinel organisms across the Eastern Mediterranean and the Black Sea

Tsangaris

Moschino

Strogyloudi

et al. 2015

Environ Sci Pollut Res

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“…Next, 50 µL of EDTA solution (4 mM EDTA-2Na, 1 M HCl) and 1000 µL of Ellman solution (2 mM DTNB (5,5'-dithio-bis(2-nitrobenzoic acid), 0.2 M sodium phosphate, 2 M NaCl) were added to the tubes mixed and centrifuged (3000 g for 5 min) for absorbance determination at 412 nm. Sulfhydryl content was determined by comparison to a glutathione (GSH) curve, and metallothionein concentration was calculated considering the metallothionein cysteine content described for mussels of 30% (Viarengo et al, 1997 with minor modifications).…”

Section: Determination Of Metallothionein Concentrationmentioning

confidence: 99%

Localization of Inorganic and Organic Mercury in the Liver and Kidney of Cyprinus carpio by Autometallography

Prodocimo

Neto²,

Grötzner³

et al. 2024

Ecotoxicol. Environ. Contam.

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Mercury bioaccumulation in different tissues largely depends upon metal chemical form and has important implications on tissue-specific toxicity. Then, simple tools for mercury tissue localization are very useful for understanding the toxicity of mercurial compounds in fishes. In the present study, inorganic and organic mercury distribution was investigated in carp Cyprinus carpio, utilizing autometallography. Juvenile C. carpio were exposed through force-feeding with mercury contaminated pelleted food at doses of 0.5 µg g -1 of Hg +2 or MeHg twice at 15 days interval, and killed 15 days after the second exposure for sampling. Organic mercury was observed inside hepatocytes and in biliary ducts of liver, whereas inorganic form of mercury was observed only in biliary ducts. In the kidney, mercury was observed mainly in renal tubules, with the predominance of inorganic over organic mercury. The present study demonstrated that autometallography is a useful tool for the tissue localization of two mercurial compounds in the liver and kidney of C. carpio after trophic exposure.Keywords: Autometallography, mercury, liver, kidney, Cyprinus carpio. Localização do mercúrio inorgânico e orgânico em fígado e rim de Cyprinus carpio através da autometalografia ResumoA bioacumulação de mercúrio em diferentes tecidos depende da forma química do metal e tem implicações importantes na toxicidade de tecidos específicos. Deste modo, ferramentas simples para a localização de mercúrio em tecidos são úteis para a compreensão da toxicidade de compostos mercuriais em peixes. No presente estudo, a distribuição de mercúrio inorgânico e orgânico foi investigada em carpa Cyprinus carpio, utilizando autometalografia. Exemplares juvenis de C. carpio foram expostos através de alimentação forçada com pellets de ração contaminada com mercúrio em doses de 0.5 µg g -1 Hg +2 ou MeHg duas vezes em um intervalo de quinze dias, e sacrificados 15 dias após a segunda exposição para amostragem. O mercúrio orgânico foi observado no interior dos hepatócitos e nos ductos biliares. No rim, o mercúrio foi observado principalmente nos túbulos renais, com predominância do mercúrio inorgânico sobre o orgânico. O presente estudo demonstrou que a autometalografia é uma ferramenta útil para a localização tecidual de dois compostos mercuriais em fígado e rim de C. carpio após exposição trófica.Palavras-chave: Autometalografia, mercúrio, fígado, rim, Cyprinus carpio.

show abstract

A simple spectrophotometric method for metallothionein evaluation in marine organisms: an application to Mediterranean and Antarctic molluscs

Cited by 801 publications

References 30 publications

Multi-biomarker responses as indication of contaminant effects in Gambusia affinis from impacted rivers by municipal effluents

Multi-biomarker responses as indication of contaminant effects in Gambusia affinis from impacted rivers by municipal effluents

Biochemical biomarker responses to pollution in selected sentinel organisms across the Eastern Mediterranean and the Black Sea

Localization of Inorganic and Organic Mercury in the Liver and Kidney of Cyprinus carpio by Autometallography

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