A simple Streptomyces spore-based impedimetric biosensor to detect lindane pesticide

Rodriguez, María Lorena López; Benimeli, Claudia Susana; Madrid, Rossana E.; Giacomelli, Carla E.

doi:10.1016/j.snb.2014.10.030

Cited by 20 publications

(6 citation statements)

References 74 publications

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“…Although, there is no standard method for determining immunosensor calibration curve [44], usually the changes in model elements extracted from fitting of raw impedance data to an equivalent model are reported as the sensor output [45]. Occasionally, the impedance at a particular frequency is also used.…”

Section: Calibration Curvementioning

confidence: 99%

Adjunction of Avidin to a Cysteamine Self-Assembled Monolayer for Impedimetric Immunosensor

Ouerghi¹,

Diouani²,

Belkacem³

et al. 2016

JBNB

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In this work an impedimetric immunosensor based on affinity immobilization method of a biotin labelled anti-human IgG antibody, used as a model system, was reported. The experimental procedure involves the growth of a self-assembled monolayer of a thiol (cysteamine) carrying terminal amine groups on gold electrodes. Glutarardehyde, a homobifunctional cross-linker, was used as a coupling reagent for the covalent linking of avidin to the amine groups of cysteamine. The attachment of the biotin labeled antibodies (anti-Human IgG) to the subsequent modified gold electrode was achieved by affinity interactions tacking advantage of the strong avidin-biotin bridge. The stepwise assembly process of the electrode was interrogated by means of cyclic voltammetry, electrochemical impedance spectroscopy and contact angle measurements. The response of the antibody modified electrode to their target antigens was investigated in the presence of BSA (bovin serum albumin) in order to alleviate non-specific adsorption problems. A proposed electrical model was used to analyse the experimental data. The resulting immunosensor has a linear dynamic range of 100-900 ng•ml −1 of antigen and a detection limit of 100 ng•ml −1 .

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Section: Calibration Curvementioning

confidence: 99%

Adjunction of Avidin to a Cysteamine Self-Assembled Monolayer for Impedimetric Immunosensor

Ouerghi¹,

Diouani²,

Belkacem³

et al. 2016

JBNB

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“…Its well-established neurotoxicity, carcinogenicity, and consequent health risks led to a worldwide ban of lindane by the Stockholm Convention in 2007 [68,69]. In fact, some countries are still using it for economic reasons [30,64]. Further, it is still found in ecological niches such as water bodies and in crops, resulting in major environmental problems [68].…”

Section: Electrochemical Detection Of Lindanementioning

confidence: 99%

“…The biosensor itself was based on the activity of Steptomyces strain M7, which utilizes lindane as the carbon source for growth. The biosensor reached a detection limit of 0.03 µM [30]. Table 2 summarizes electrochemical methods and main performance characters reported for the determination of lindane at different electrodes.…”

Section: Electrochemical Detection Of Lindanementioning

confidence: 99%

“…The technique uses the principle of creating a negative imprint of the targeted molecule on the electrode surface to achieve better selectivity and sensitivity [27,28]. Another approach that has recently been gaining attention is to immobilize microorganisms on the electrode surfaces and monitor the interaction between these organisms when they get in contact with the targeted analyte [29,30]. An alternative method is to modify the electrode surfaces with nanomaterials such as carbon nanotubes or manganese dioxide nanotubes which will result in increased surface area, increased conductivity and hence better response [31,32].…”

Section: Introductionmentioning

confidence: 99%

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Recent Development on the Electrochemical Detection of Selected Pesticides: A Focused Review

Noori¹,

Mortensen

Geto³

2020

Sensors

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Pesticides are heavily used in agriculture to protect crops from diseases, insects, and weeds. However, only a fraction of the used pesticides reaches the target and the rest slips through the soil, causing the contamination of ground-and surface water resources. Given the emerging interest in the on-site detection of analytes that can replace traditional chromatographic techniques, alternative methods for pesticide measuring have recently encountered remarkable attention. This review gives a focused overview of the literature related to the electrochemical detection of selected pesticides. Here, we focus on the electrochemical detection of three important pesticides; glyphosate, lindane and bentazone using a variety of electrochemical detection techniques, electrode materials, electrolyte media, and sample matrix. The review summarizes the different electrochemical studies and provides an overview of the analytical performances reported such as; the limits of detection and linearity range. This article highlights the advancements in pesticide detection of the selected pesticides using electrochemical methods and point towards the challenges and needed efforts to achieve electrochemical detection suitable for on-site applications.

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“…The low frequency effects (<1000 Hz) show how capacitance changes to the impedance occur over a range of frequencies. 29,30 Formation of the PRSV immunocomplex modulates the electrical double layer. These changes typically occur at low frequencies as the behavior of the double layer is modulated by the ionic charge distribution within the double layer.…”

Section: Biosensor Development For the Detection Of Prsv Coat Proteinsmentioning

confidence: 99%

The detection of papaya ringspot virus coat protein using an electrochemical immunosensor

et al. 2016

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A simple Streptomyces spore-based impedimetric biosensor to detect lindane pesticide

Cited by 20 publications

References 74 publications

Adjunction of Avidin to a Cysteamine Self-Assembled Monolayer for Impedimetric Immunosensor

Adjunction of Avidin to a Cysteamine Self-Assembled Monolayer for Impedimetric Immunosensor

Recent Development on the Electrochemical Detection of Selected Pesticides: A Focused Review

The detection of papaya ringspot virus coat protein using an electrochemical immunosensor

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