A Simplified Bacterial Spore Stain

Bartholomew, J. W.; Mittwer, Tod

doi:10.3109/10520295009110979

Cited by 69 publications

(43 citation statements)

References 5 publications

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“…Although many workers pointed out that the cell size increased with every budding (7)(8)(9)(10), and the size of cells bearing various numbers of bud scars presented by YANAGITA et al3, the actual size as a function of the cellular divisional ages does not appear in the literature.…”

Section: Discussionmentioning

confidence: 87%

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Increase in Cell Size as a Measure of Growth of Saccharomyces Cerevisiae

Hayashibe

Sando

Abe

1973

J. Gen. Appl. Microbiol.

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Section: Discussionmentioning

confidence: 87%

“…On the other hand, it was pointed out by a number of workers that the surface area of the budding yeast cells gradually increased with repeated budding (7)(8)(9)(10).…”

mentioning

confidence: 99%

Increase in Cell Size as a Measure of Growth of Saccharomyces Cerevisiae

Hayashibe

Sando

Abe

1973

J. Gen. Appl. Microbiol.

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“…The following gram-positive organisms were obtained from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (http://www.dsmz.de), were grown in the media indicated, and were tested to determine their abilities to produce FuBA: Corynebacterium flavescens DSM20296 (medium M53), Corynebacterium glutamicum DSM20300 (medium M53), Dietzia maris DSM43672 (medium M65), Dietzia papillomatosis DSM44961 (medium M65), Gordonia amarae DSM43392 (medium M65), Gordonia hydrophobica DSM44015 (medium M535), Mycobacterium avium DSM44156 (medium M645), Mycobacterium phlei DSM43239 (medium M645), Nocardia asteroides DSM43757 (liquid standing culture in medium M65), Nocardia polyresistens DSM44932 (medium M65), Tsukamurella paurometabola DSM20162 (medium M53), Tsukamurella spumae DSM44113 (medium M65), Williamsia maris DSM44693 (medium M65), Williamsia muralis DSM44343 (medium M535), Actinospica acidiphila DSM44926 (medium M987), Bacillus mycoides DSM2048 (medium M1), Enterococcus aquimarinus DSM17690 (medium M92), Geodermatophilus obscurus DSM43160 (medium M65), Streptosporangium cinnabarium DSM44094 (medium M547), and Streptomyces coelicolor DSM40233 (medium M65). The presence of spores was detected by malachite staining (5).…”

Section: Methodsmentioning

confidence: 99%

Widespread Abundance of Functional Bacterial Amyloid in Mycolata and Other Gram-Positive Bacteria

Jordal

Dueholm

Larsen

et al. 2009

Appl Environ Microbiol

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Until recently, extracellular functional bacterial amyloid (FuBA) has been detected and characterized in only a few bacterial species, including Escherichia coli, Salmonella, and the gram-positive organism Streptomyces coelicolor. Here we probed gram-positive bacteria with conformationally specific antibodies and revealed the existence of FuBA in 12 of 14 examined mycolata species, as well as six other distantly related species examined belonging to the phyla Actinobacteria and Firmicutes. Most of the bacteria produced extracellular fimbriae, sometimes copious amounts of them, and in two cases large extracellular fibrils were also produced. In three cases, FuBA was revealed only after extensive removal of extracellular material by saponification, indicating that there is integrated attachment within the cellular envelope. Spores of species in the genera Streptomyces, Bacillus, and Nocardia were all coated with amyloids. FuBA was purified from Gordonia amarae (from the cell envelope) and Geodermatophilus obscurus, and they had the morphology, tinctorial properties, and ␤-rich structure typical of amyloid. The presence of approximately 9-nm-wide amyloids in the cell envelope of G. amarae was visualized by transmission electron microscopy analysis. We conclude that amyloid is widespread among gram-positive bacteria and may in many species constitute a hitherto overlooked integral part of the spore and the cellular envelope.

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“…The isolates were also studied for their colony morphology, cell shape and gram reaction as per the standard procedure given by Barthalomew and Mittewar (1950) and Anonymous (1957). The isolates were subjected to biochemical characterization by employing the standard procedures given by Cappuccino and Sherman (1992).…”

Section: Characterization Of Fp Isolatesmentioning

confidence: 99%