A simplified point-of-care testing approach for preeclampsia blood biomarkers based on nanoscale field effect transistors

Pham, Thuy T.; Tran, Duy Phu; Nguyen, Minh Cong; Amen, Mohamed T.; Winter, Marnie; Whitehead, Clare; Toh, Justine; Thierry, Benjamin

doi:10.1039/d1nr02461b

Cited by 14 publications

(14 citation statements)

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“…The dilution factor was taken into consideration when calculating the limit of detection for the nucleocapsid protein in blood. The indium oxide nanoribbon FET sensors were fabricated as previously described and integrated within the 3D-printed fluidic device. , All FET measurements were performed using a Keysight Precision Source/Measure Unit B2900A Series (Keysight Technologies, USA). The data were recorded and interpreted via Keysight EasyEXPERT Software (Keysight Technologies, USA).…”

Section: Methodsmentioning

confidence: 99%

“…The indium oxide nanoribbon FET sensors were fabricated as previously described and integrated within the 3D-printed fluidic device. 5,6 All FET measurements were performed using a Keysight Precision Source/Measure Unit B2900A Series (Keysight Technologies, USA). The data were recorded and…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

“…35,37,56 The indium oxide FET sensors were fabricated and biofunctionalized with antibodies against the SARS-CoV-2 nucleocapsid protein as previously reported. 6,16 Finger-pricked blood samples were collected from healthy volunteers after obtaining their written consent (Ethic number 203167 at the University of South Australia's Human Research Ethics Committee). Immediately after the blood collection, the samples were diluted with isotonic glucose solution spiked with different concentrations of the SARS-CoV-2 nucleocapsid protein and then introduced into the sampling inlet of the PoC nanoscale FET biosensor.…”

Section: Efficiency Of the Washingmentioning

confidence: 99%

“…Advances in biosensing technologies are paving the way to the feasibility of performing advanced bioanalytical assays in PoC settings. Among the various biosensing technologies relevant to PoC testing, label-free electrical biosensors, especially those based on nanoscale field-effect transistors (FETs), present significant advantages including ultrahigh sensitivity (typically in the sub-picomolar range), , fast and quantitative measurement capability (in the order of minutes), direct signal transduction, low power consumption, and compatibility with the multiplexed analyte measurement as well as on-chip sample processing and integration. − These features suggest the feasibility of FET sensor-based PoC testing with high diagnostic accuracy, reduced complexity, increased portability, and acceptable cost. , …”

Section: Introductionmentioning

confidence: 99%

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Integrated Platform Addressing the Finger-Prick Blood Processing Challenges of Point-of-Care Electrical Biomarker Testing

et al. 2022

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Continued advances in label-free electrical biosensors pave the way to simple, rapid, cost-effective, high-sensitivity, and quantitative biomarker testing at the point-of-care setting that would profoundly transform healthcare. However, implementation in routine diagnostics is faced with significant challenges associated with the inherent requirement for biofluid sample processing before and during testing. We present here a simple yet robust autonomous finger-prick blood sample processing platform integrated with nanoscale field-effect transistor biosensors and demonstrate the feasibility of measuring the SARS-CoV-2 nucleocapsid protein. The 3Dprinted platform incorporates a high-yield blood-to-plasma separation module and a delay valve designed to terminate the assay at a specific time. The platform is driven by hydrostatic pressure to efficiently and automatically dispense plasma and washing/measurement buffer to the nanosensors. Our model study demonstrates the feasibility of detecting down to 1.4 pg/mL of the SARS-CoV-2 nucleocapsid protein within 25 min and with only minimal operator intervention.

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Section: Methodsmentioning

confidence: 99%

Section: ■ Experimental Sectionmentioning

confidence: 99%

Section: Efficiency Of the Washingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Integrated Platform Addressing the Finger-Prick Blood Processing Challenges of Point-of-Care Electrical Biomarker Testing

et al. 2022

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“…In this field, nanotechnology was also applied with different intents: (1) to improve diagnostic management and (2) to implement treatment strategies. New nanotechnology-based point-of-care tests are being developed to improve the assessment of preeclampsia development risk [ 97 , 98 , 99 ]. For instance, recent advancements have been made in the development of electrochemical immunosensors for the early clinical diagnosis of preeclampsia within the 18–20th gestational weeks [ 100 ].…”

Section: Preeclampsia and Fetal Growth Restrictionmentioning

confidence: 99%

Nanotechnologies in Obstetrics and Cancer during Pregnancy: A Narrative Review

Bertozzi

Corradetti

Seriau

et al. 2022

JPM

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Nanotechnology, the art of engineering structures on a molecular level, offers the opportunity to implement new strategies for the diagnosis and management of pregnancy-related disorders. This review aims to summarize the current state of nanotechnology in obstetrics and cancer in pregnancy, focusing on existing and potential applications, and provides insights on safety and future directions. A systematic and comprehensive literature assessment was performed, querying the following databases: PubMed/Medline, Scopus, and Endbase. The databases were searched from their inception to 22 March 2022. Five independent reviewers screened the items and extracted those which were more pertinent within the scope of this review. Although nanotechnology has been on the bench for many years, most of the studies in obstetrics are preclinical. Ongoing research spans from the development of diagnostic tools, including optimized strategies to selectively confine contrast agents in the maternal bloodstream and approaches to improve diagnostics tests to be used in obstetrics, to the synthesis of innovative delivery nanosystems for therapeutic interventions. Using nanotechnology to achieve spatial and temporal control over the delivery of therapeutic agents (e.g., commonly used drugs, more recently defined formulations, or gene therapy-based approaches) offers significant advantages, including the possibility to target specific cells/tissues of interest (e.g., the maternal bloodstream, uterus wall, or fetal compartment). This characteristic of nanotechnology-driven therapy reduces side effects and the amount of therapeutic agent used. However, nanotoxicology appears to be a significant obstacle to adopting these technologies in clinical therapeutic praxis. Further research is needed in order to improve these techniques, as they have tremendous potential to improve the accuracy of the tests applied in clinical praxis. This review showed the increasing interest in nanotechnology applications in obstetrics disorders and pregnancy-related pathologies to improve the diagnostic algorithms, monitor pregnancy-related diseases, and implement new treatment strategies.

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Recent Advances in 1D Photonic Crystals: Diverse Morphologies and Distinctive Structural Colors for Multifaceted Applications

Kou,

Zhang,

2024

Advanced Optical Materials

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Abstract1D photonic crystals (1DPCs) with hierarchically structured lamellar periodic frameworks that enable precise control of light‐matter interactions and contribute robust structural colors represent a groundbreaking advancement in optical materials. The microstructural characteristics and the constituent material properties of these photonic materials play pivotal roles in determining their optical performance and functionality. In recent years, a diverse array of novel 1D photonic structures crafted from various materials emerged, showcasing their tremendous potential in advanced applications. This article provides an in‐depth review of the recent developments in 1DPCs, emphasizing their morphological designs, fabrication strategies, and optical applications. In detail, 1DPCs featuring distinct geometrical morphologies, including lamellar, helical, fibrous, spherical, and nanochained structures are systematically introduced, highlighting the unique optical properties arising from their periodic microstructures. Then, various fabrication methods, involving some innovative techniques utilizing standing‐wave optics, UV dual photopolymerization, and inkjet printing, are succinctly summarized for constructing different photonic structures by using diverse building materials. Subsequently, some typical application examples of 1D photonic materials are listed and discussed involving visual sensing, intelligent displays, anti‐counterfeiting technology, photonic pigments, and optical devices. Finally, the passage addresses the current challenges and presents a forward‐looking perspective on the future fabrication and application of 1DPCs.

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A simplified point-of-care testing approach for preeclampsia blood biomarkers based on nanoscale field effect transistors

Abstract: Rapid diagnosis of preeclampsia is necessary to ensure timely administration of appropriate care and prevent the potentially catastrophic complications of the condition affecting both mothers and babies. While the diagnostic...

Cited by 14 publications

References 54 publications

Integrated Platform Addressing the Finger-Prick Blood Processing Challenges of Point-of-Care Electrical Biomarker Testing

Integrated Platform Addressing the Finger-Prick Blood Processing Challenges of Point-of-Care Electrical Biomarker Testing

Nanotechnologies in Obstetrics and Cancer during Pregnancy: A Narrative Review

Recent Advances in 1D Photonic Crystals: Diverse Morphologies and Distinctive Structural Colors for Multifaceted Applications

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