A simplified procedure for measuring the class of anti-bacterial antibodies by mixed reverse passive antiglobulin haemagglutination (MRPAH)

Eggert, F M; Edebo, L.; Gurner, B.W.; Coombs, R.R.A.

doi:10.1016/0022-1759(79)90076-0

Cited by 24 publications

(9 citation statements)

References 7 publications

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“…This is the way we updated antiglobulin tests on bacteria [20, 34, 35] so that end points are read by haemagglutination and not by bacterial agglutination. The antibody-sensitized bacteria are, after washing, reacted with red cells coupled with antiglobulin.…”

Section: The Antiglobulin Reaction Is 50 Years Oldmentioning

confidence: 99%

Historical Note: Past, Present and Future of the Antiglobulin Test

Coombs

1998

Vox Sanguinis

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This is a review of two invited talks on the antiglobulin reaction on the occasion of the 50th Anniversaries of the International Blood Group Reference Laboratory, now at Bristol, and the National Blood Service, London, and coincidentally the 51st anniversary of the antiglobulin test! The first talk (as specially requested) is a very personal reminiscence of the discovery, with Rob Race and Arthur Mourant, of the antiglobulin test in blood group serology. The second talk traces developments in antiglobulin testing in general immunology using, of course, isotype-specific antiglobulin reagents as and when they became available. Special emphasis was given to: (1) testing for poorly agglutinating bacterial antibodies; (2) special procedures for measuring reaginic (IgE) antibodies to various allergens; (3) the incorporation of an antiglobulin step or stage in many of the routine automated immunoassay procedures; (4) the special experimental procedures in the form of mixed antiglobulin reactions to reveal antibodies to the surface antigens on tissue cells, and, finally, (5) by chemically coupling antiglobulin to red blood cells, a distinctive integrated immunoassay system was developed, enabling reverse passive haemagglutination as an assay for immunoglobulins, rosetting reactions to reveal native IgG receptors on lymphocytes or antibody immunoglobulin reacting with CD marker antigens; this same reagent, namely red-cell-labelled antiglobulin, can be used to detect antibodies reacting with either bacteria or antigens adsorbed on the walls of wells of microtitre plates. The need for improved stabilization and preservation of the antiglobulin-linked red cells to prolong their ‘shelf-life’ is stressed.

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Section: The Antiglobulin Reaction Is 50 Years Oldmentioning

confidence: 99%

Historical Note: Past, Present and Future of the Antiglobulin Test

Coombs

1998

Vox Sanguinis

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“…The bulk of salivary and colostral rabbit E agglutination results from the activity of 1 IS IgA [Eggert, 1977] but [Eggert, 1977;Eggert et al, 1979], In both saliva and amniotic fluid the SB A F for 5. mutans serotype c was higher than the SB A F for other streptococci.…”

Section: Levels O F Activities In Normal Whole Saliva and Amniotic Fluidmentioning

confidence: 99%

The Nature of Secretory Agglutinins and Aggregating Factors

Eggert¹

1980

Int Arch Allergy Immunol

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Secretory conglutinin-like factor (SKF), secretory bacterial aggregating factors (SBAF) and 11S secretory IgA antibodies of human saliva and full term amniotic fluid were quantitated by microtitration and partially characterized. The SBAF of both saliva and amniotic fluid aggregated a variety of oral streptococci, but no secretory IgA antibodies were found in amniotic fluid. The SKF and SBAF are distinguished from IIS IgA antibodies by being inhibited with EDTA and by being more susceptible to inhibition with reducing agents. These active factors are also distinguished from submaxillary mucins. Components of normal serum inhibit both SKF and SBAF, but blood-group reactive sugars cannot block either SKF or SBAF.

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“…The sensitivity of the PRIST system is quoted as 0.5 IU/ml (1.2 ng/ml) by Pharmacia. Coombs et al [1978] and Eggert et al [1979] have shown that mixed reverse pas sive antiglobulin haemagglutination (MrPAH) is a most sensitive method for measurement of classes of anti-bacterial an tibodies. The antiglobulin is linked to an in dicator red cell by the chromic chloride method.…”

Section: Introductionmentioning

confidence: 99%

Measurement of Human Serum IgE and IgA by Reverse Passive Antiglobulin Haemagglutination

Scott¹,

Thornley²,

Coombs³

et al. 1981

Int Arch Allergy Immunol

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Serum IgE levels can be measured by reverse passive antiglobulin haemagglutination (RPAH) of trypsin-treated human or sheep red cells coupled to sheep IgG anti-human IgE by chromic chloride. The results show a high correlation with those obtained by the radioactive single radial immunodiffusion method. Interfering anti-sheep IgG factors can be easily removed by absorption with small amounts of whole sheep or bovine serum cross-linked with glutaraldehyde. Standardisation with the British standard for IgE shows that the detection limit of the RPAH method is 0.5 IU/ml (1.2 ng/ml). The system is therefore comparable in sensitivity to the paper radio-immunosorbent test, and has the advantages of being simple, rapid and cheap. The RPAH method can be used to measure any class of immunoglobulin. For IgA the detection limit is found to be 10^––⁴ IU/ml (1.4ng/ml).

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A simplified procedure for measuring the class of anti-bacterial antibodies by mixed reverse passive antiglobulin haemagglutination (MRPAH)

Cited by 24 publications

References 7 publications

Historical Note: Past, Present and Future of the Antiglobulin Test

Historical Note: Past, Present and Future of the Antiglobulin Test

The Nature of Secretory Agglutinins and Aggregating Factors

Measurement of Human Serum IgE and IgA by Reverse Passive Antiglobulin Haemagglutination

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