A simplified, rapid LC-MS/MS assay for serum and salivary creatinine

Bernstone, Laura; Jayanti, Anuradha; Keevil, Brian

doi:10.1016/j.clinms.2018.11.004

Cited by 4 publications

(1 citation statement)

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“…Colorimetric assays using enzymatic and Jaffé methods are frequently used in clinical practice. However, these methods are prone to interference from hemolysis, lipemia, bilirubin, glucose, hemoglobin, and creatine 2 , 3 . The low-cost Jaffé method is still widely used.…”

Section: Introductionmentioning

confidence: 99%

Assessment of creatinine concentration in whole blood spheroids using paper spray ionization–tandem mass spectrometry

Sham

Badu‐Tawiah

McWilliam

et al. 2022

Sci Rep

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Accurate quantification of blood creatinine is important to estimate the glomerular filtration rate. Existing techniques using liquid chromatography tandem mass spectrometry (LC–MS/MS) have a high accuracy and eliminate most interferences encountered in routine enzymatic and Jaffé methods. However, they require laborious and time-consuming sample treatment and data acquisition. The aim of this study is to develop a fast and simple method to enable a direct analysis of whole blood creatinine with performance measures that are comparable to conventional LC–MS/MS. 5μL whole blood is formed as a three-dimensional spheroid on hydrophobic silanized paper substrates which then undergoes paper-spray ionization—tandem mass spectrometry (PSI–MS/MS). The method is validated using real human samples and compared with LC–MS/MS. PSI–MS/MS whole blood analysis exhibited a lower limit of quantification of 2.5 μg/mL, precision ≤ 6.3%, recovery in the range of 88–94% and excellent linearity (R2 > 0.99; 2.5—20 μg/mL) covering the normal range for creatinine levels. Creatinine levels were comparable to those measured by LC–MS/MS with small deviations of less than 0.3 μg/mL. This simple, fast and accurate microsampling technique for direct analysis of creatinine from whole blood shows promise for routine clinical screening and monitoring. This approach can be readily extended for other analytes of interest and, due to inherent advantages relating to cost, storability, speed, and simplicity, it can be especially advantageous for use in resource-limited settings.

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Section: Introductionmentioning

confidence: 99%