A simplified technique for embryo biopsy for preimplantation genetic diagnosis

Kaskar, K.; Gill, Jimmy; DeSplinter, Traci

doi:10.1016/j.fertnstert.2007.06.093

Cited by 14 publications

(10 citation statements)

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“…Various methods exist to achieve this goal, most of which require lots of practice and experience (5,9). We have found that the displacement method is very easy to learn and does not require a lot of additional training (8). In addition, this method allows for the rapid removal of the blastomere, thereby limiting the exposure of the embryo to Ca/Mg free biopsy medium.…”

Section: Discussionmentioning

confidence: 99%

“…However, this increases patients' cost and is inconvenient for both the host laboratory and the outside technician. By using the current displacement biopsy method, the technician can use less time and fewer embryos for practice before performing biopsy on patient's embryos (8).…”

Section: Discussionmentioning

confidence: 99%

“…A less traumatic displacement method has been developed for biopsy of human day 3 embryos, which involves a zona opening and the introduction of medium into the perivitelline space (PVS) causing the blastomere to be extruded through the opened zona (7,8). This displacement method of obtaining blastomeres is simple and easy to learn.…”

mentioning

confidence: 99%

“…This displacement method of obtaining blastomeres is simple and easy to learn. Most importantly, this method significantly reduces the biopsy time and does not negatively impact the blastomere integrity or the subsequent development of the embryo to the blastocyst stage (8). However, a direct comparison has not been done between the two methods in terms of embryo development, pregnancy, and implantation.…”

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confidence: 99%

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Comparison of development and implantation of human embryos biopsied with two different methods: aspiration and displacement

Wang

Kaskar

Ren

et al. 2009

Fertility and Sterility

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Comparison of development and implantation of human embryos biopsied with two different methods: aspiration and displacement

Wang

Kaskar

Ren

et al. 2009

Fertility and Sterility

Self Cite

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“…Various methods have been employed, such as partial zona dissection, Acidic Tyrode’s solution, or the laser assisted hatching system [17–20]. Each method has its own risks and benefits.…”

Section: Introductionmentioning

confidence: 99%

A simple, less invasive stripper micropipetter-based technique for day 3 embryo biopsy

Cedillo¹,

Ocampo-Bárcenas²,

Maldonado³

et al. 2016

Fertil Res and Pract

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BackgroundPreimplantation genetic screening (PGS) is an important procedure for in vitro fertilization (IVF). A key step of PGS, blastomere removal, is abundant with many technical issues. The aim of this study was to compare a more simple procedure based on the Stipper Micropipetter, named S-biopsy, to the conventional aspiration method.MethodsOn Day 3, 368 high-quality embryos (>7 cells on Day3 with <10% fragmentation) were collected from 38 women. For each patient, their embryos were equally separated between the conventional method (n = 188) and S-biopsy method (n = 180). The conventional method was performed using a standardized protocol. For the S-biopsy method, a laser was used to remove a significantly smaller portion of the zona pellucida. Afterwards, the complete embryo was aspirated with a Stripper Micropipetter, forcing the removal of the blastomere. Selected blastomeres went to PGS using CGH microarrays. Embryo integrity and blastocyst formation were assessed on Day 5. Differences between groups were assessed by either the Mann-Whitney test or Fisher Exact test.ResultsBoth methods resulted in the removal of only one blastomere. The S-biopsy and the conventional method did not differ in terms of affecting embryo integrity (95.0% vs. 95.7%) or blastocyst formation (72.7% vs. 70.7%). PGS analysis indicated that aneuploidy rate were similar between the two methods (63.1% vs. 65.2%). However, the time required to perform the S-biopsy method (179.2 ± 17.5 s) was significantly shorter (5-fold) than the conventional method.ConclusionThe S-biopsy method is comparable to the conventional method that is used to remove a blastomere for PGS, but requires less time. Furthermore, due to the simplicity of the S-biopsy technique, this method is more ideal for IVF laboratories.Electronic supplementary materialThe online version of this article (doi:10.1186/s40738-016-0027-4) contains supplementary material, which is available to authorized users.

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Integrating Preimplantation Genetic Diagnosis into the ART Laboratory

Drury

2010

Reproductive Endocrinology and Infertility

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A simplified technique for embryo biopsy for preimplantation genetic diagnosis

Cited by 14 publications

References 10 publications

Comparison of development and implantation of human embryos biopsied with two different methods: aspiration and displacement

Comparison of development and implantation of human embryos biopsied with two different methods: aspiration and displacement

A simple, less invasive stripper micropipetter-based technique for day 3 embryo biopsy

Integrating Preimplantation Genetic Diagnosis into the ART Laboratory

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