A single-cell based mass cytometry study on heterogeneous interactions between upconversion nanoparticles and human immune cells

Gerelkhuu, Zayakhuu; Perumalsamy, Haribalan; Maddahfar, Mahnaz; Jin, Dong-Yan; Song, Jaewoo; Yoon, Tae Hyun

doi:10.1039/d2en00821a

Environ. Sci.: Nano

2023

DOI: 10.1039/d2en00821a

|View full text |Cite

A single-cell based mass cytometry study on heterogeneous interactions between upconversion nanoparticles and human immune cells

Zayakhuu Gerelkhuu

Haribalan Perumalsamy

Mahnaz Maddahfar

et al.

Abstract: To encourage the use of lanthanide-doped upconversion nanoparticles (UCNPs) in a wide range of biomedical applications, it is necessary to perform comprehensive assessments of their toxicity. Particularly, the cellular association...

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

References 49 publications

(69 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

Overcoming the age-dependent SARS-CoV-2 vaccine response through hybrid immunity: analysis of humoral and cellular immunity with mass cytometry profiling

Gerelkhuu,

Park,

Lee

et al. 2024

Immun Ageing

View full text Add to dashboard Cite

Background Age-dependent immune responses to coronavirus disease 2019 (COVID-19) vaccinations and breakthrough infections (BIs) in young and middle-aged individuals are unclear. Methods This nationwide multicenter prospective cohort study analyzed immune responses in participants of the ChAdOx1 (ChAd)-ChAd-mRNA vaccine group using cytometry by time-of-flight, anti-spike protein antibody (Sab) and anti-nucleocapsid antibody (Nab) titers, plaque reduction neutralization tests (PRNTs), and interferon-gamma (IFN-γ) release assays at various time points. Results We evaluated 347 participants with an average age of 38.9 ± 9.4 years (range: 21–63). There was a significant inverse correlation between age and Sab levels after the second dose (slope − 14.96, P = 0.032), and this was more pronounced after the third dose (slope − 208.9, P < 0.001). After BIs, older participants showed significantly higher Sab titers (slope 398.8, P = 0.001), reversing the age-related decline observed post-vaccination. This reversal was also observed in PRNTs against wild-type SARS-CoV-2 and the BA.1 and BA.5 variants. IFN-γ responses increased markedly after the third dose and Bis, but showed a weak positive correlation with age, without statistical significance. Immune cell profiling revealed an age-dependent decrease in the proportions of B-cell lineage cells. The proportions of naive CD4+ and CD8+ T cells were inversely correlated with age, whereas the proportions of mature T cell subsets with memory function, including memory CD4+ T, CD8+ TEM, CD8+ TEMRA, and TFH cells, increased with age. Conclusions Age-dependent waning of the serologic response to COVID-19 vaccines occurred even in middle-aged individuals, but was reversed after BIs. IFN-γ responses were preserved, compensating for the decrease in naive T cell populations, with an increase in memory T cell populations.

show abstract

Overcoming the age-dependent SARS-CoV-2 vaccine response through hybrid immunity: analysis of humoral and cellular immunity with mass cytometry profiling

Gerelkhuu,

Park,

Lee

et al. 2024

Immun Ageing

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

A single-cell based mass cytometry study on heterogeneous interactions between upconversion nanoparticles and human immune cells

Abstract: To encourage the use of lanthanide-doped upconversion nanoparticles (UCNPs) in a wide range of biomedical applications, it is necessary to perform comprehensive assessments of their toxicity. Particularly, the cellular association...

Cited by 1 publication

References 49 publications

Overcoming the age-dependent SARS-CoV-2 vaccine response through hybrid immunity: analysis of humoral and cellular immunity with mass cytometry profiling

Overcoming the age-dependent SARS-CoV-2 vaccine response through hybrid immunity: analysis of humoral and cellular immunity with mass cytometry profiling

Contact Info

Product

Resources

About