A single-cell landscape of triptolide-associated testicular toxicity in mice

Zhang, Wei; Xia, Siyu; Ou, Jinhuan; Cao, Min; Cheng, Guangqing; Li, Zhijie; Wang, Jigang; Yang, Chuanbin

doi:10.1016/j.jpha.2023.04.006

Cited by 10 publications

(1 citation statement)

References 44 publications

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“…Compared with the bulk RNA-seq method that determines RNA information of mixed cell population, single-cell RNA sequencing (scRNA-seq) enables comprehensive detection of the transcriptional information of individual cells, expanding our understanding of cellular heterogeneity. [14][15][16][17] Therefore, scRNA-seq advanced our knowledge of biological processes and toxicology studies at a high resolution. For instance, scRNA-seq has been used to dissect liver injury and diseases in both human and animal models, [18][19][20][21] providing comprehensive insights into the underlying mechanisms in different cell types of liver tissues.…”

Section: Introductionmentioning

confidence: 99%

Insight into Tetrabromobisphenol A‐Associated Liver Transcriptional Landscape via Single Cell RNA Sequencing

Bai,

Zhu,

et al. 2023

Advanced Biology

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In recent years, there has been growing concern over the rising incidence of liver diseases, with increasing exposure to environmental toxins as a significant contributing factor. However, the mechanisms of liver injury induced by environmental pollutants are largely unclear. Here, using tetrabromobisphenol A (TBBPA), a widely used brominated flame retardant, as an example, environmental toxin‐induced liver toxicity in mice is characterized via single‐cell sequencing technology. Heterogeneous gene expression profiles after exposure to TBBPA in major cell types of the liver are demonstrated. In hepatocytes, pathway analysis of differentially expressed genes reveals the enhanced interferon response and diminished metabolic processes. The disrupted endothelial functions in TBBPA‐treated cells are then shown. Moreover, the activation of M2‐polarization in Kupffer cells, as well as activated effector T and B cells are unveiled in TBBPA‐treated cells. Finally, ligand‐receptor pair analysis shows that TBBPA disrupts cell‐cell communication and induces an inflammatory microenvironment. Overall, the results reveal that TBBPA‐induced dysfunction of hepatocytes and endothelial cells may then activate and recruit other immune cells such as Kuffer cells, and T/NK cells into the liver, further increasing inflammatory response and liver injury. Thus, the results provide novel insight into undesiring environmental pollutant‐induced liver injury.

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Section: Introductionmentioning

confidence: 99%

Insight into Tetrabromobisphenol A‐Associated Liver Transcriptional Landscape via Single Cell RNA Sequencing

Bai,

Zhu,

et al. 2023

Advanced Biology

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A Single‐Cell Transcriptome Profiling of Triptolide‐Induced Nephrotoxicity in Mice

Wu,

Guo,

Xia

et al. 2024

Advanced Biology

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Triptolide (TP), an active component isolated from the traditional Chinese herb Tripterygium wilfordii Hook F (TWHF), shows great promise for treating inflammation‐related diseases. However, its potential nephrotoxic effects remain concerning. The mechanism underlying TP‐induced nephrotoxicity is inadequately elucidated, particularly at single‐cell resolution. Hence, single‐cell RNA sequencing (scRNA‐seq) of kidney tissues from control and TP‐treated mice is performed to generate a thorough description of the renal cell atlas upon TP treatment. Heterogeneous responses of nephron epithelial cells are observed after TP exposure, attributing differential susceptibility of cell subtypes to excessive reactive oxygen species and increased inflammatory responses. Moreover, TP disrupts vascular function by activating endothelial cell immunity and damaging fibroblasts. Severe immune cell damage and the activation of pro‐inflammatory Macro_C1 cells are also observed with TP treatment. Additionally, ligand‐receptor crosstalk analysis reveals that the SPP1 (osteopontin) signaling pathway targeting Macro_C1 cells is triggered by TP treatment, which may promote the infiltration of Macro_C1 cells to exacerbate renal toxicity. Overall, this study provides comprehensive information on the transcriptomic profiles and cellular composition of TP‐associated nephrotoxicity at single‐cell resolution, which can strengthen the understanding of the pathogenesis of TP‐induced nephrotoxicity and provide valuable clues for the discovery of new therapeutic targets to ameliorate TP‐associated nephrotoxicity.

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Triptolide induces apoptosis and cytoprotective autophagy by ROS accumulation via directly targeting peroxiredoxin 2 in gastric cancer cells

Chen,

Zhong,

Song

et al. 2024

Cancer Letters

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A single-cell landscape of triptolide-associated testicular toxicity in mice

Cited by 10 publications

References 44 publications

Insight into Tetrabromobisphenol A‐Associated Liver Transcriptional Landscape via Single Cell RNA Sequencing

Insight into Tetrabromobisphenol A‐Associated Liver Transcriptional Landscape via Single Cell RNA Sequencing

A Single‐Cell Transcriptome Profiling of Triptolide‐Induced Nephrotoxicity in Mice

Triptolide induces apoptosis and cytoprotective autophagy by ROS accumulation via directly targeting peroxiredoxin 2 in gastric cancer cells

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