2022
DOI: 10.1101/2022.09.28.509835
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A Single-Cell Level Comparison of Human Inner Ear Organoids and the Human Cochlea and Vestibular Organs

Abstract: Genetic inner ear disorders are among the most common congenital abnormalities and lead to hearing loss and balance disorders. Ideally, tissue culture models of the inner ear should contain a functional unit combining otic sensory and nonsensory cell types to recapitulate the varied etiologies of inner ear disorders. Here, we evaluated cell type diversity of late-stage human pluripotent stem cell-derived inner ear organoids using single-cell transcriptomic analysis, electron microscopy and immunohistochemistry… Show more

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Cited by 2 publications
(1 citation statement)
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“…According to previous studies 26,27 , we annotated cell types based on the known cell-type-specific markers (Supplementary Fig. 1a), including Tympanic border cell (TBC, Emilin2 + ) 28 , Fibroblast cell (FC, Coch + , Otos + ) 29,30 , Inner phalangeal cell/Inner border cell (IPhC/IBC, Slc1a3 + , S100a6 + ) [31][32][33] , Deiter cell/Pillar cell/Inner sulcus cell/Outer sulcus cell (DC/PC/ ISC/OSC, Gata3 + , Fbxo2 + , Skp1a + , Epyc + , Bmp4 + ) 26,34-37 , Reissner's membrane cell (RMC, Vom1 + ) 38 , Capillary endothelial cells (CEC, Ly6c1 + ) 39 , Capillary pericytes (Rgs5 + ) 30 , OHC (Ocm + , Slc26a5 + ) 40,41 , and IHC (Otof + , Slc17a8 + ) 25,42 . The marker genes expressed in each cluster are listed in Supplementary Data 1.…”
Section: Resultsmentioning
confidence: 99%
“…According to previous studies 26,27 , we annotated cell types based on the known cell-type-specific markers (Supplementary Fig. 1a), including Tympanic border cell (TBC, Emilin2 + ) 28 , Fibroblast cell (FC, Coch + , Otos + ) 29,30 , Inner phalangeal cell/Inner border cell (IPhC/IBC, Slc1a3 + , S100a6 + ) [31][32][33] , Deiter cell/Pillar cell/Inner sulcus cell/Outer sulcus cell (DC/PC/ ISC/OSC, Gata3 + , Fbxo2 + , Skp1a + , Epyc + , Bmp4 + ) 26,34-37 , Reissner's membrane cell (RMC, Vom1 + ) 38 , Capillary endothelial cells (CEC, Ly6c1 + ) 39 , Capillary pericytes (Rgs5 + ) 30 , OHC (Ocm + , Slc26a5 + ) 40,41 , and IHC (Otof + , Slc17a8 + ) 25,42 . The marker genes expressed in each cluster are listed in Supplementary Data 1.…”
Section: Resultsmentioning
confidence: 99%