A single enzyme PCR-RFLP protocol targeting 16S rRNA/tRNAval region to authenticate four commercially important shrimp species in India

Wilwet, Lidiya; Jeyasekaran, G.; Shakila, R. Jeya; Sivaraman, Balasubramanian; Padmavathy, P.

doi:10.1016/j.foodchem.2017.06.132

Cited by 29 publications

(10 citation statements)

References 19 publications

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“…However, these methods also exhibit some limitations, such as complex procedures, poor stability, and poor reproducibility (Chapela et al, 2007;Larraín, Díaz, Lamas, Uribe, & Araneda, 2014;Rasmussen & Morrissey, 2009). The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique, which was developed based on fingerprinting technology, is most widely used for species identification because of its simple procedure, low cost, and good reproducibility (Sivaraman et al, 2018;Wilwet, Jeyasekaran, Shakila, Sivaraman, & Padmavathy, 2018). Moreover, it just needs PCR thermocycler and electrophoresis apparatus, which means most laboratories could bear the cost, that is also a realistic factor in most developing countries.…”

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confidence: 99%

Methodology and application of PCR‐RFLP for species identification in tuna sashimi

et al. 2020

Food Science & Nutrition

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The Thunnini, or tuna, comprise many species with very different commercial values. The principal raw tuna product on the market is sashimi, for which the species used is difficult to identify through conventional morphological analysis. The present study amplified the cytochrome b gene ( Cytb ) of 4 major tuna species used for preparing sashimi—yellowfin tuna ( Thunnus albacares ), southern bluefin tuna ( Thunnus maccoyii ), bigeye tuna ( Thunnus obesus ), and Atlantic bluefin tuna ( Thunnus thynnus )—and 4 species commonly mislabeled as components of tuna sashimi—albacore tuna ( Thunnus alalunga ), skipjack tuna ( Katsuwonus pelamis ), striped marlin ( Tetrapturus audax ), and swordfish ( Xiphias gladius ). Polymerase chain reaction (PCR) amplicons were digested with 5 restriction enzymes— Eco 147 I, Hinf I, Mbo I, Xag I, and Hind II—to obtain characteristic restriction maps of the above‐mentioned raw tuna species and the commonly mislabeled species. An identification method using PCR restriction fragment length polymorphism (PCR‐RFLP) was established and validated using 39 commercial tuna sashimi samples, which verified that this method provides results consistent with those obtained by classical sequencing. PCR‐RFLP has several advantages over classical sequencing, such as simplicity, speed and accuracy. This technique could support species identification for raw tuna and sashimi.

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confidence: 99%

Methodology and application of PCR‐RFLP for species identification in tuna sashimi

et al. 2020

Food Science & Nutrition

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“…This method can be applied as a tool for improving mislabeling issues, traceability, and authentication. Determination of beef‐jerky species variation, authentication of camel meat and shrimp species are some recent applications of PCR‐RFLP method 44–46 . In the present study, sequencing analysis of coi gene of fish eggs, and digestion with selected restriction enzymes was performed and proved to be efficient for authentication purposes.…”

Section: Discussionmentioning

confidence: 86%

Potential biological markers by DNA‐based tools for determination of Greek PDO geographical origin and authenticity: “Avgotaracho Mesolonghiou” and “Vostizza currant”

et al. 2021

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Background: Food traceability and authentication had become mandatory for food industry and global food trade. Numerous DNA-based methods could contribute against food frauds, because of their advantages such as simplicity, accuracy, and robustness.The aim of this study was to explore whether unique biological markers for two high valuable and popular Greek protected designation of origin (PDO) products could be indicated. For this purpose, "Avgotaracho Mesolonghiou" known as Greek caviar and "Vostizza" currant were subjected to DNA-based analysis. PCR-RAPD, PCR-RFLP, and PCR-DGGE were performed for Greek PDO products and potential biological markers were explored, based on either genomic DNA or bacteria communities.Results: Band profiles resulted in molecular techniques, could be used as a "barcode" to certify the origin and authenticity of PDO products. Conclusion:These methods are proposed, as alternative traceability tools, in order to provide unique markers and could be the key to "farm to table" challenge. Therefore, biological markers could be used throughout the entire commercial supply chain and protect food products, which hold a quality scheme, from adulterations.

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“…The 16 S rDNA is an efficient marker for discrimination among different shrimp species. For examples, Wilwet, Jeyasekaran, Shakila, Sivaraman, and Padmavathy (2018) could efficiently use it to differentiate L. vannamei , Penaeus monodon , P. semisulcatus , and Fenneropenaeus indicus . Moreover, Sharma, Watts, and Singh (2020) used this gene for discrimination of the penaeids L. vannamei , P. duorarum , P. monodon , L. setiferus , and Pleoticus muelleri .…”

Section: Discussionmentioning

confidence: 99%

Molecular tools for assuring human health and environment-friendly frozen shellfish products in the United Arab Emirates markets

Galal-Khallaf

Abdelbaset-Donya

Hamza

et al. 2021

Food Chemistry: Molecular Sciences

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A single enzyme PCR-RFLP protocol targeting 16S rRNA/tRNAval region to authenticate four commercially important shrimp species in India

Cited by 29 publications

References 19 publications

Methodology and application of PCR‐RFLP for species identification in tuna sashimi

Methodology and application of PCR‐RFLP for species identification in tuna sashimi

Potential biological markers by DNA‐based tools for determination of Greek PDO geographical origin and authenticity: “Avgotaracho Mesolonghiou” and “Vostizza currant”

Molecular tools for assuring human health and environment-friendly frozen shellfish products in the United Arab Emirates markets

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