A single nucleotide polymorphism in the BART promoter region of Epstein-Barr virus isolated from nasopharyngeal cancer cells

Kim, Hyoji; Burassakarn, Ati; Yuting, Kang; Iizasa, Hisashi; Yoshiyama, Hironori

doi:10.1016/j.bbrc.2019.10.028

Cited by 12 publications

(6 citation statements)

References 31 publications

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“…We have recently reported a characteristic SNP in the promoter of BART that increases BART promoter activity. This SNP is frequently detected in EBV-associated NPC with an odds ratio of 5.7 (55). Therefore, studying the promoter of BART and the SNPs associated with it can help develop strong candidates that suppress BART transcription.…”

Section: Single Nucleotide Polymorphisms (Snps) In the Viral Mirna Prmentioning

confidence: 99%

Role of Viral and Host microRNAs in Immune Regulation of Epstein-Barr Virus-Associated Diseases

et al. 2020

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Epstein-Barr virus (EBV) is an oncogenic human herpes virus that was discovered in 1964. Viral non-coding RNAs, such as BamHI-A rightward fragment-derived microRNAs (BART miRNAs) or BamHI-H rightward fragment 1-derived miRNAs (BHRF1 miRNA) in EBV-infected cells have been recently reported. Host miRNAs are also upregulated upon EBV infection. Viral and host miRNAs are important in maintaining viral infection and evasion of host immunity. Although miRNAs in EBV-infected cells often promote cell proliferation by targeting apoptosis or cell cycle, this review focuses on the regulation of the recognition of the host immune system. This review firstly describes the location and organization of two clusters of viral miRNAs, then describes evasion from host immune surveillance systems by modulating viral gene expression or inhibiting innate and acquired immunity by viral miRNAs as well as host miRNAs. Another topic is the enigmatic depletion of viral miRNAs in several types of EBV-infected tumor cells. Finally, this review introduces the strong correlation of nasopharyngeal cancer cases with a newly identified single nucleotide polymorphism that enhances BART miRNA promoter activity.

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Section: Single Nucleotide Polymorphisms (Snps) In the Viral Mirna Prmentioning

confidence: 99%

Role of Viral and Host microRNAs in Immune Regulation of Epstein-Barr Virus-Associated Diseases

et al. 2020

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“…LMP1 conferred apoptotic resistance by inhibiting the DNA damage response and pro-apoptotic protein level synthesis by activating cellular pathways, especially the PI3K/Akt pathway [33][34][35]. In addition to LMP1, EBV-miRNAs also inhibit apoptosis of cancer cells [36,37]. EBV-miR-BART4 and EBV-miR-BART5-3p inhibited apoptosis by targeting genes, such as PTEN and p53 [38,39].…”

Section: Discussionmentioning

confidence: 99%

Epstein–Barr Virus Infection of Oral Squamous Cells

et al. 2020

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The Epstein–Barr virus (EBV) is a human herpesvirus associated with various cancers. The number of reports that describe infection of EBV in oral squamous carcinoma cells is increasing. However, there is no available in vitro model to study the possible role of EBV in the development of oral squamous cell carcinoma. Herein, we report establishment of a latent EBV infection of well-differentiated HSC1 cells and poorly differentiated SCC25 cells. Viral copy numbers per cell in EBV-infected HSC1 and SCC25 cells are 2 and 5, respectively. Although the EBV copy number was small, spontaneous viral replication was observed in EBV-infected HSC1 cells. Contrarily, infectious viral production was not observed in EBV-infected SCC25 cells, despite containing larger number of EBV genomes. Chemical activation of cells induced expression of viral lytic BZLF1 gene in EBV-infected HSC1 cells, but not in EBV-infected SCC25 cells. EBV infection activated proliferation and migration of HSC1 cells. However, EBV-infection activated migration but not proliferation in SCC25 cells. In conclusion, EBV can infect squamous cells and establish latent infection, but promotion of cell proliferation and of lytic EBV replication may vary depending on stages of cell differentiation. Our model can be used to study the role of EBV in the development of EBV-associated oral squamous cell carcinoma.

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“…Numerous studies revealed that various genes involved in GC are targets of EBV miRNAs and are pro-tumorigenic, some of which are detailed in Table 3. Particular attention is paid to miR-BART5-3p, and miR-BART1-5p in EBVaGC [79], since they were found to bind and facilitate degradation of the important tumor suppressor P53 mRNA [80]. Besides, miR-BART1-5 and miR-BART9 are involved in cell growth and migration by inducing the reduction of important proteins like E-cadherin, which are necessary to maintain epithelial cell cohesion [81,82].…”

Section: Ebv Latency and Epigenetic Activitymentioning

confidence: 99%

Overview of Epstein–Barr-Virus-Associated Gastric Cancer Correlated with Prognostic Classification and Development of Therapeutic Options

Brisotto

Repetto

et al. 2020

IJMS

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Gastric cancer (GC) is a deadly disease with poor prognosis that is characterized by heterogeneity. New classifications based on histologic features, genotypes, and molecular phenotypes, for example, the Cancer Genome Atlas subtypes and those by the Asian Cancer Research Group, help understand the carcinogenic differences in GC and have led to the identification of an Epstein–Barr virus (EBV)-related GC subtype (EBVaGC), providing new indications for tailored treatment and prognostic factors. This article provides a review of the features of EBVaGC and an update on the latest insights from EBV-related research with a particular focus on the strict interaction between EBV infection and the gastric tumor environment, including the host immune response. This information may help increase our knowledge of EBVaGC pathogenesis and the mechanisms that sustain the immune response of patients since this mechanism has been demonstrated to offer a survival advantage in a proportion of patients with GC.

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A single nucleotide polymorphism in the BART promoter region of Epstein-Barr virus isolated from nasopharyngeal cancer cells

Cited by 12 publications

References 31 publications

Role of Viral and Host microRNAs in Immune Regulation of Epstein-Barr Virus-Associated Diseases

Role of Viral and Host microRNAs in Immune Regulation of Epstein-Barr Virus-Associated Diseases

Epstein–Barr Virus Infection of Oral Squamous Cells

Overview of Epstein–Barr-Virus-Associated Gastric Cancer Correlated with Prognostic Classification and Development of Therapeutic Options

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