A single nucleotide polymorphism in the promoter region of let-7 family is associated with lung cancer risk in Chinese

Abstract

ABSTRACT. Lung cancer is a complex polygenic disease and many genetic factors are involved in the development of the disease. As one of the most important and widely studied families of microRNA, let-7 appears to play an important role in initiation and progression of lung cancer. Any small changes in miRNA level or its target point can cause significant changes in gene function. In this study, we examined whether a single-nucleotide polymorphism in the promoter region of the let-7 family (rs10877887) is associated with the susceptibility to L.Q. Shen et al. 4506©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 4505-4512 (2015) and prognosis of lung adenocarcinoma cancer. A hospital-based casecontrol research model was used in our study. The single-nucleotide polymorphism was genotyped in 69 lung cancer patients and 75 healthy controls by direct sequencing. The correlation between rs10877887 genotypes and the susceptibility to lung cancer was evaluated using an unconditional logistic regression model. Populations with the CT+CC genotype had a significantly increased AC risk compared to those with the TT genotype (CT+CC vs TT: P = 0.043, OR = 2.032, 95%CI = 1.018-4.054). Furthermore, the risk effect was greater in subgroups of females over 60 years old (CT+CC vs TT: OR = 6.857, 95%CI = 1.425-33.008, P = 0.012), and the C allele were confirmed to be a risk factor related to lung cancer in these females (P = 0.012). The singlenucleotide polymorphism rs10877887 in the promoter region of the let-7 family was found to be responsible for the susceptibility to lung adenocarcinoma cancer in Chinese individuals. This association was significantly stronger in females who were more than 60 years old.

Down-regulation of let-7 microRNA increased K-ras expression in lung damage induced by radon

“…More recently, Shen et al (2015) reported that the single-nucleotide polymorphism in the promoter region of let-7 family is strongly associated with susceptibility to lung adenocarcinoma in Chinese individuals, especially in females who are more than 60 years old. In addition, aberrant expression of let-7 has also been observed in many other human malignancies (Ma et al, 2014), with a distinct expression pattern in cancer development (Boyerinas et al, 2010;Wagner et al, 2014).…”

Genetic polymorphisms of miRNA let7a‐2 and pri‐mir‐34b/c are associated with an increased risk of papillary thyroid carcinoma and clinical/pathological features

“…Wang et al demonstrated that the let7a‐2 rs10877887 polymorphism might be associated with a lower risk of PTC and the rs13293512 polymorphism could be correlated with lymph node metastasis in patients with PTC . In addition, several reports have described the association between let‐7 family polymorphisms and different cancers, such as head and neck cancer, hepatocellular carcinoma, and lung cancer …”

A single nucleotide polymorphism in the promoter region (rs10877887) of let-7 is associated with hepatocellular carcinoma in a Chinese population

“…Although the effect of let-7 on cancer and its mechanism involves a number of processes, SNPs are believed to play a prominent role in all. Two SNPs identified in the promoter region of let-7 have been associated with hepatocellular carcinoma and lung cancer susceptibility (Shen et al, 2015).…”