A Single Standard to Determine Multi-Components Method Coupled with Chemometric Methods for the Quantification, Evaluation and Classification of Notopterygii Rhizoma et Radix from Different Regions

Abstract

An ultra-high-performance liquid chromatography-quadrupole/time of flight mass spectrometry is used to identify 33 compounds in Notopterygii rhizoma and radix, after which a single standard to determine multi-components method is established for the simultaneous determination of 19 compounds in Notopterygii rhizoma and radix using chlorogenic acid and notopterol as the internal standard. To screen the potential chemical markers among Notopterygii rhizoma and radix planted in its natural germination area and in others, the quantitative data of 19 compounds are analyzed via partial least-squares discriminant analysis (PLS–DA). Depending on the variable importance parameters (VIP) value of PLS–DA, six compounds are selected to be the potential chemical markers for the discrimination of Notopterygii rhizoma and radix planted in the different regions. Furthermore, the Fisher’s discriminant analysis is used to build the models that are used to classify Notopterygii rhizoma and radix from the different regions based on the six chemical markers. Experimental results indicate that Notopterygii rhizoma and radix planted in the Sichuan province are distinguished successfully from those in other regions, reaching a 96.0% accuracy rating. Therefore, a single standard to determine multi-components method combined with a chemometrics method, which contains the advantages such as simple, rapid, economical and accurate identification, offers a new perspective for the quantification, evaluation and classification of Notopterygii rhizoma and radix from the different regions.

Database-aided UHPLC-Q-orbitrap MS/MS Strategy Putatively Identifies 52 Compounds from Wushicha Granule to Propose Anti-counterfeiting Quality-markers for Pharmacopoeia

“…According to the principle, one Q-marker candidate should not be easily obtained via industrialization. Therefore, 9 identi ed compounds were rstly excluded as Q-marker candidate, including D-gluconic acid (1), quinic acid (2), gallic acid (3), protocatechuic acid (4), methyl gallate (6), linoleic acid (46), palmitic acid (47), oleic acid (48), ethyl palmitate (49), and ethyl stearate (50). This is because these natural compounds could be industrially synthesized as well [75],[76], [77], [78, 79], [79][80][81] .…”

Fingerprint analysis of phenolic acid extract of Salvia miltiorrhiza by digital reference standard analyzer with one or two reference standards

“…Quantitative methods include the relative correction factor method [ 9 – 12 ] and the quantitative ERS technique [ 9 – 11 ]. These methods not only promote the application of multi-components determination and fingerprint analysis for quality control of medicines but also have been proven to be more economical and simple [ 13 – 25 ]. However, the substitute RS method used in the holistic quality control of medicines still has some problems.…”

Fingerprint Analysis of Phenolic Acid Extract of Salvia Miltiorrhiza by Digital Reference Standard Analyzer With One or Two Reference Standards

“…Quantitative methods include the relative correction factor method [9][10][11][12] and the quantitative ERS technique [9][10][11]. These methods not only promote the application of multi-components determination and ngerprint analysis for quality control of medicines but also have been proven to be more economical and simple [13][14][15][16][17][18][19][20][21][22][23][24][25]. However, the substitute RS method used in the holistic quality control of medicines still has some problems.…”