A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses

Priye, Aashish; Bird, Sara W.; Light, Yooli Kim; Ball, Christopher; Negrete, Oscar; Meagher, Robert J.

doi:10.1038/srep44778

Cited by 260 publications

(231 citation statements)

References 46 publications

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“…Furthermore, in this study, positive and negative controls were used in all the reactions to assure not having contamination problems. The advantage of observing the results by real-time allows the diagnosis in less than 30 min, so that treatment and care to avoid transmission of the disease can start immediately [23]. Studies already reported ZIKV transmission through sexual [2], perinatal [3] and blood transfusion [4].…”

Section: Discussionmentioning

confidence: 99%

Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP)

Castro

Sabalza

Barber

et al. 2018

Journal of Oral Microbiology

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Background: Zika virus (ZIKV) is a single-stranded RNA virus and member of the Flaviviridae family. Recent studies have reported that saliva can be an important alternative to detect ZIKV. Saliva requires less processing than blood greatly simplifying the assay. Loop-mediated Isothermal Amplification (LAMP) is a rapid assay that detects nucleic acids, including ZIKV RNA. Aim: The aim of this study was to evaluate the efficacy of saliva and urine to diagnose ZIKV infection in subjects during the acute phase, through ZIKV RNA detection by LAMP. Method: A total of 131 samples (68 saliva and 63 urine samples) from 69 subjects in the acute phase of ZIKV infection, and confirmed positive for ZIKV by blood analysis through real time-PCR, were collected and analyzed by Reverse Transcriptase Loop-mediated Isothermal Amplification (RT-LAMP). Results: From the 68 saliva samples, 45 (66.2%) were positive for ZIKV with an average time to positivity (Tp) of 13.5 min, and from the 63 urine samples, 25 (39.7%) were positive with the average Tp of 15.8 min. Saliva detected more samples (p = 0.0042) and had faster Tp (p = 0.0176) as compared with urine. Conclusion: Saliva proved to be a feasible alternative to diagnose ZIKV infection during the acute phase by LAMP.

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Section: Discussionmentioning

confidence: 99%

Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP)

Castro

Sabalza

Barber

et al. 2018

Journal of Oral Microbiology

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“…[4] While reverse transcription PCR (RT-PCR) is often used as aN AT,R T-LAMP has also been extensively used for virus detection owing to its robustness,h igh sensitivity and specificity,s hort incubation time,a nd simplified thermal management. [7][8][9][10][11][12][13][14] Although RT-LAMP allows virus samples to be used for amplification without sample preparation, the small sample volume and dilution steps involved decrease detection sensitivity. [7][8][9][10][11][12][13][14] Although RT-LAMP allows virus samples to be used for amplification without sample preparation, the small sample volume and dilution steps involved decrease detection sensitivity.…”

mentioning

confidence: 99%

Valve‐Enabled Sample Preparation and RNA Amplification in a Coffee Mug for Zika Virus Detection

et al. 2018

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The recent outbreaks of Zika virus (ZIKV) infection represent ap ublic health challenge.R apid, cost-effective,a nd reliable diagnostic tools for ZIKV detection at the point of care (POC) are highly desirable,e specially for resource-limited nations.T oaddress the need, we have developed an integrated device to achieve sample-to-answer ZIKV detection. The device features innovative ball-based valves enabling the storage and sequential delivery of reagents for virus lysis and apaper-based unit for RNAenrichmentand purification. The paper unit is placed in ac ommercially available coffee mug that provides ac onstant temperature for reverse transcription loop-mediated isothermal amplification (RT-LAMP), followed by colorimetric detection by naked eye or ac ellphone camera. Using the device,w ed emonstrated the reproducible detection of ZIKV in human urine and saliva samples.Supportinginformation and the ORCID identification number(s) for the author(s) of this article can be found under: https://doi.

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“…Automating the S value calculation can be an alternative way of improving PAD sensitivity. Many previous studies have demonstrated the benefits of a smartphone for reading and obtaining a quantitative and objective result for an accurate diagnostic . For the first version of the PAD, an Android‐based application was developed on a smartphone for reproducible and quantitative results from the raw signals.…”

Section: Discussionmentioning

confidence: 99%

Detection of chikungunya virus‐specific IgM on laser‐cut paper‐based device using pseudo‐particles as capture antigen

Theillet

Grard

Galla

et al. 2019

Journal of Medical Virology

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The incidence of arbovirus infections has increased dramatically in recent decades, affecting hundreds of millions of people each year. The Togaviridae family includes the chikungunya virus (CHIKV), which is typically transmitted by Aedes mosquitoes and causes a wide range of symptoms from flu‐like fever to severe arthralgia. Although conventional diagnostic tests can provide early diagnosis of CHIKV infections, access to these tests is often limited in developing countries. Consequently, there is an urgent need to develop efficient, affordable, simple, rapid, and robust diagnostic tools that can be used in point‐of‐care settings. Early diagnosis is crucial to improve patient management and to reduce the risk of complications. A glass‐fiber laser‐cut microfluidic device (paper‐based analytical device [PAD]) was designed and evaluated in a proof of principle context, for the analysis of 30 µL of patient serum. Biological raw materials used for the functionalization of the PAD were first screened by MAC‐ELISA (IgM capture enzyme‐linked immunosorbent assay) for CHIKV Immunoglobulin M (IgM) capture and then evaluated on the PAD using various human samples. Compared with viral lysate traditionally used for chikungunya (CHIK) serology, CHIKV pseudo‐particles (PPs) have proven to be powerful antigens for specific IgM capture. The PAD was able to detect CHIKV IgM in human sera in less than 10 minutes. Results obtained in patient sera showed a sensitivity of 70.6% and a specificity of around 98%. The PAD showed few cross‐reactions with other tropical viral diseases. The PAD could help health workers in the early diagnosis of tropical diseases such as CHIK, which require specific management protocols in at‐risk populations.

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A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses

Cited by 260 publications

References 46 publications

Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP)

Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP)

Valve‐Enabled Sample Preparation and RNA Amplification in a Coffee Mug for Zika Virus Detection

Detection of chikungunya virus‐specific IgM on laser‐cut paper‐based device using pseudo‐particles as capture antigen

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