A smartphone-based fiber-optic aptasensor for label-free detection of <i>Plasmodium falciparum</i> glutamate dehydrogenase

Sanjay, M; Singh, Naveen K.; Ngashangva, Lightson; Goswami, Pranab

doi:10.1039/c9ay02406a

Cited by 29 publications

(12 citation statements)

References 45 publications

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“…The performances reached in this paper still leave room for improvements, especially to achieve more user-friendly interfaces. However, the many assets of OFs and their ease of implementation would enable substantial alternatives to the traditional equipment currently used at a research level, for instance, their combination with smartphones and portable devices, the elaboration of online bioprofiling databases [30].…”

Section: Discussion Of the Performances And Tunability Of The Lab-on-fiber Technologymentioning

confidence: 99%

“…This original experimental study on Plasmodium falciparum cultures could open the door towards the integration of these OF probes into smaller biochips or bio-sticks connected to smartphone or specifically designed microfluidic packages. This could be of great interest for an innovative, portable and cost-effective diagnosis method [30][31][32].…”

Section: Introductionmentioning

confidence: 99%

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PfHRP2 detection using plasmonic optrodes: performance analysis

Loyez

Wells

Hambye

et al. 2021

Malar J

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Background Early malaria diagnosis and its profiling require the development of new sensing platforms enabling rapid and early analysis of parasites in blood or saliva, aside the widespread rapid diagnostic tests (RDTs). Methods This study shows the performance of a cost-effective optical fiber-based solution to target the presence of Plasmodium falciparum histidine-rich protein 2 (PfHRP2). Unclad multimode optical fiber probes are coated with a thin gold film to excite Surface Plasmon Resonance (SPR) yielding high sensitivity to bio-interactions between targets and bioreceptors grafted on the metal surface. Results Their performances are presented in laboratory conditions using PBS spiked with growing concentrations of purified target proteins and within in vitro cultures. Two probe configurations are studied through label-free detection and amplification using secondary antibodies to show the possibility to lower the intrisic limit of detection. Conclusions As malaria hits millions of people worldwide, the improvement and multiplexing of this optical fiber technique can be of great interest, especially for a future purpose of using multiple receptors on the fiber surface or several coated-nanoparticles as amplifiers.

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Section: Discussion Of the Performances And Tunability Of The Lab-on-fiber Technologymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

PfHRP2 detection using plasmonic optrodes: performance analysis

Loyez

Wells

Hambye

et al. 2021

Malar J

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“…Enzymes, most of which are proteins, can be detected using aptasensor such as Plasmodium falciparum glutamate dehydrogenase (PfGDH) [ 89 , 90 ] and creatine kinase isoenzymes (CK-MB) [ 91 ]. To further increase the convenience of detection, Sanjay et al [ 92 ] constructed a multi-channel, smartphone-based optic fiber platform for quantitative detection of PfGDH. As depicted in Figure 4 b, the smartphone flashlight and camera were coupled to two ends of optic fiber by a four-channel smartphone accessory.…”

Section: Smartphone-based Aptasensormentioning

confidence: 99%

“…( b ) Schematic illustration of the smartphone-based optic fiber platform. Reprinted with permission from Ref [ 92 ], copyright 2020, RSC Publishing. ( c ) Smartphone dark box comprising (1) a bottom part fitted with a UV-LED light source, (2) a lid fitted with a replaceable smartphone adapter, and (3) a sample plate for seven lateral flow strips.…”

Section: Smartphone-based Aptasensormentioning

confidence: 99%

Applications of Smartphone-Based Aptasensor for Diverse Targets Detection

Tan

et al. 2022

Biosensors

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Aptamers are a particular class of functional recognition ligands with high specificity and affinity to their targets. As the candidate recognition layer of biosensors, aptamers can be used to sense biomolecules. Aptasensors, aptamer-based biosensors, have been demonstrated to be specific, sensitive, and cost-effective. Furthermore, smartphone-based devices have shown their advantages in binding to aptasensors for point-of-care testing (POCT), which offers an immediate or spontaneous responding time for biological testing. This review describes smartphone-based aptasensors to detect various targets such as metal ions, nucleic acids, proteins, and cells. Additionally, the focus is also on aptasensors-related technologies and configurations.

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“…In that context, new-generation of affinity molecules like aptamers are being widely preferred due to their effectiveness and robustness [ 12 ]. Aptamers have already found application in several bioassay modalities such as Quartz crystal microbalance [ 13 , 14 ], Cantilever-based biosensing [ 15 , 16 ], electrochemistry [ 17 ], Surface plasmon resonance [ 18 , 19 ] and spectrophotometric assays [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

An apta-aggregation based machine learning assay for rapid quantification of lysozyme through texture parameters

et al. 2021

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A novel assay technique that involves quantification of lysozyme (Lys) through machine learning is put forward here. This article reports the tendency of the well- documented Ellington group anti-Lys aptamer, to produce aggregates when exposed to Lys. This property of apta-aggregation has been exploited here to develop an assay that quantifies the Lys using texture and area parameters from a photograph of the elliptical aggregate mass through machine learning. Two assay sets were made for the experimental procedure: one with high Lys concentration between 25–100 mM and another with low concentration between 1–20 mM. The high concentration set had a sample volume of 10 μl while the low concentration set had a higher sample volume of 100 μl, in order to obtain the statistical texture values reliably from the aggregate mass. The platform exhibited an experimental limit of detection of 1 mM and a response time of less than 10 seconds. Further, two potential operating modes for the aptamer were hypothesized for this aggregation property and the more accurate mode among the two was ascertained through bioinformatics studies.

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A smartphone-based fiber-optic aptasensor for label-free detection of Plasmodium falciparum glutamate dehydrogenase

Abstract: A novel smartphone-based, multi-channel, optic fiber platform for quantitative detection of Plasmodium falciparum glutamate dehydrogenase (PfGDH) has been explored in this study.

Cited by 29 publications

References 45 publications

PfHRP2 detection using plasmonic optrodes: performance analysis

PfHRP2 detection using plasmonic optrodes: performance analysis

Applications of Smartphone-Based Aptasensor for Diverse Targets Detection

An apta-aggregation based machine learning assay for rapid quantification of lysozyme through texture parameters

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