A solid-phase enzyme immunoassay for serum ferritin.

Abstract

We describe an enzyme immunoassay for human serum ferritin in which antibody adsorbed on polystyrene tubes is used. Adsorbed gamma-globulins against human ferritin were first allowed to react with ferritin and a second antiferritin antibody, labeled with alkaline phosphatase, was added. The amount of bound enzyme/antibody conjugate was proportional to the ferritin titer in the assay. This method offers stable reagents that can be kept for many months at 4 degrees C. The average values for ferritin in normal men and women were, respectively, 58 and 43 microgram/liter. The lowest detectable concentration was 5 microgram/liter.

Performance and comparability of laboratory methods for measuring ferritin concentrations in human serum or plasma: A systematic review and meta-analysis

“…Eleven thousand eight hundred ninety three records were obtained from the search strategy depicted in Table 1 . From 252 records extracted, 187 studies were included in the qualitative analysis [ 8 , 10 , 15 – 199 ] and 148 in the meta-analysis [ 8 , 10 , 15 , 17 , 18 , 22 , 23 , 26 , 28 , 30 – 32 , 34 – 56 , 58 – 64 , 66 – 68 , 72 , 73 , 75 , 77 – 80 , 82 – 85 , 87 – 92 , 94 – 98 , 100 – 102 , 106 – 109 , 111 – 116 , 120 – 137 , 139 – 143 , 145 , 146 , 148 – 151 , 153 – 157 , 159 – 164 , 166 – 175 , 177 , 179 – ...…”

“…The first reported methods for ferritin determination were radioactive, using radioisotopes to label the antigen (IRMA) or the antibody (RIA) [ 16 , 61 ]. Common colorimetric methods include peroxidase or glycosidase based color development [ 177 , 204 , 205 ]. Methods based in agglutination and light passage could be turbidimetric or nephelometric [ 35 , 206 ].…”