A somatic cell hybrid panel for pig regional gene mapping characterized by molecular cytogenetics

Yerle, Martine M.; Echard, G.; Robic, Annie; Mairal, Aline; Dubut-Fontana, C.; Riquet, Juliette; Pinton, Philippe; Milan, Denis; Lahbib-Mansais, Y.; Gellin, Joël

doi:10.1159/000134338

Cited by 279 publications

(188 citation statements)

References 5 publications

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“…The Minnesota porcine Radiation Hybrid (IMpRH) panels (26) was used to map porcine chemerin. Primer pair P5-P6 complementary to intron 4 of chemerin, was designed to obtain an 182 bp fragment.…”

Section: Chromosome Localization By Radiation Hybrid Mappingmentioning

confidence: 99%

Cloning of porcine chemerin, ChemR23 and GPR1 and their involvement in regulation of lipogenesis

Huang¹,

Zhang²,

Lei³

et al. 2010

BMB Reports

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Chemerin is a novel adipokine which is abundant in adipose tissue to promote adipocyte differentiation and with significant relativity to BMI and insulin sensitivity. We report here the molecular characterization of porcine chemerin and its receptors ChemR23 and GPR1, as well as their transcriptional regulation during lipogenesis. Chemerin was mainly expressed in liver, intestine, kidney and adipose tissue, consistent with the expression pattern of GPR1, but not ChemR23, which was predominantly present in spleen and temperately in adipose tissue. We further investigated the lipogenesis-related transcriptional activation of PPARγ and KLF15 on chemerin and its receptors. The data showed that KLF15, but not PPARγ, can up-regulate the mRNA level of chemerin, ChemR23 and GPR1, which was consistent with the results of luciferase assay that confirmed the effect of KLF15 on ChemR23 promoter. Taken together, our data provide basic molecular information for the further investigation on the function of chemerin in lipogenesis. [BMB reports 2010; 43(7): 491-498]

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Section: Chromosome Localization By Radiation Hybrid Mappingmentioning

confidence: 99%

Cloning of porcine chemerin, ChemR23 and GPR1 and their involvement in regulation of lipogenesis

Huang¹,

Zhang²,

Lei³

et al. 2010

BMB Reports

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“…To localize the genes for SP-A and SP-D on the porcine genome, two PCRs were developed to amplify the porcine surfactant genes from a porcine/ rodent somatic cell hybrid panel of 27 cell lines (35). The PCRs were optimized for temperature, magnesium concentration, and the number of cycles to specifically amplify the porcine gene only.…”

Section: Chromosomal Localization Of Sp-a and Sp-dmentioning

confidence: 99%

Porcine Lung Surfactant Protein D: Complementary DNA Cloning, Chromosomal Localization, and Tissue Distribution

Eijk

Haagsman

Skinner

et al. 2000

The Journal of Immunology

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Porcine organs and lung surfactant have medically important applications in both xenotransplantation and therapy. We have started to characterize porcine lung surfactant by cloning the cDNA of porcine surfactant protein D (SP-D). SP-D and SP-A are important mediators in innate immune defense for the lung and possibly other mucosal surfaces. Porcine SP-D will also be an important reagent for use in existing porcine animal models for human lung infections. The complete cDNA sequence of porcine SP-D, including the 5′ and 3′ untranslated regions, was determined from two overlapping bacteriophage clones and by PCR cloning. Three unique features were revealed from the porcine sequence in comparison to SP-D from other previously characterized species, making porcine SP-D an intriguing species addition to the SP-D/collectin family. The collagen region contains an extra cysteine residue, which may have important structural consequences. The other two differences, a potential glycosylation site and an insertion of three amino acids, lie in the loop regions of the carbohydrate recognition domain, close to the carbohydrate binding region and thus may have functional implications. These variations were ruled out as polymorphisms or mutations by confirming the sequence at the genomic level in four different pig breeds. Porcine SP-D was shown to localize primarily to the lung and with less abundance to the duodenum, jejunum, and ileum. The genes for SP-D and SP-A were also shown to colocalize to a region of porcine chromosome 14 that is syntenic with the human and murine collectin loci.

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“…The PCR conditions were 95 °C for 5 min and 34 cycles of 94 °C for 30 s, 56-65 °C for 30 s and 72 °C for 20 s, and a final extension of 72 °C for 5 min. The PCR results for the five porcine SLC2A genes were analyzed by the tools provided at http://www.toulouse.inra.fr/lgc/pig/ hybrid.htm for INRA-SCHP and http://imprh.toulouse.inra.fr/ for IMpRH mapping [2,3] .…”

Section: Somatic Cell Hybrid and Radiation Hybrid Mappingmentioning

confidence: 99%

Assignment of solute carrier family 2 (facilitated glucose transporter), members SLC2A2, SLC2A3, SLC2A5, SLC2A8 and SLC2A12 to porcine chromosomes by somatic cell and radiation hybrid panel mapping (Brief report)

et al. 2007

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Abstract. The transport of glucose plays an important role in cellular glucose homeostasis and metabolism [1]. Due to the hydrophilic character of glucose, the transport of glucose in and out of cells requires specific carrier proteins. The mammalian facilitative glucose transport family, which contains the energy-independent transporters (gene symbol SLC2A, protein symbol GLUT), catalyzes the entry of glucose into mammalian cells by facilitative diffusion down a concentration gradient. Thirteen members of mammalian GLUT family have been now characterized [1]. In swine, the chromosomal locations for the five genes (SLC2A2, SLC2A3, SLC2A5, SLC2A8 and SLC2A12) have not yet been determined. In this study, as the first step to better understand of the roles of these GLUTs in pigs which could subsequently be beneficial for pig production, we report the mapping of the five genes using both porcine somatic cell hybrid panel (INRA-SCHP) and radiation hybrid panel (IMpRH).

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A somatic cell hybrid panel for pig regional gene mapping characterized by molecular cytogenetics

Cited by 279 publications

References 5 publications

Cloning of porcine chemerin, ChemR23 and GPR1 and their involvement in regulation of lipogenesis

Cloning of porcine chemerin, ChemR23 and GPR1 and their involvement in regulation of lipogenesis

Porcine Lung Surfactant Protein D: Complementary DNA Cloning, Chromosomal Localization, and Tissue Distribution

Assignment of solute carrier family 2 (facilitated glucose transporter), members <i>SLC2A2</i>, <i>SLC2A3</i>, <i>SLC2A5</i>, <i>SLC2A8</i> and <i>SLC2A12</i> to porcine chromosomes by somatic cell and radiation hybrid panel mapping (Brief report)

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