A specialized Hsp90 co-chaperone network regulates steroid hormone receptor response to ligand

Backe, Sarah J.; Sager, Rebecca; Regan, Bethany R; Sit, Julian; Major, Lauren A; Bratslavsky, Gennady; Woodford, Mark R.; Bourboulia, Dimitra; Mollapour, Mehdi

doi:10.1016/j.celrep.2022.111039

Cited by 23 publications

(14 citation statements)

References 83 publications

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“…The ten most significant terms (Figure ) according to biological functions (biological process, cellular component, molecular function, reactome pathway, wiki pathway) revealed that those found in cluster 1 were related to glucose metabolism; binding of sperm to zona pellucida, also found in cluster 2; HSP90-mediated steroid hormone receptor activation; HSF1-mediated controls. These results are in agreement with a higher expression profile in the zone of cysts with late spermatids as glycolysis is one of the main ATP-producing pathways, with oxidative phosphorylation, in spermatozoa and the roles of heat shock transcription factors (HSF1 and HSF2) during spermatogenesis. , HSP90-mediated activation of glucocorticoid and estrogen receptors involves a network of cochaperone proteins, which does not yet appear to have been studied during spermatogenesis. It may also be noted that the term associated with the import of peroxisomal proteins can be related to the fact that a defect in peroxisomes induces spermatogenesis arrest at the spermatid stage in mice …”

Section: Resultsmentioning

confidence: 99%

Comparative Proteome Analysis of Four Stages of Spermatogenesis in the Small-Spotted Catshark (Scyliorhinus canicula), Using High-Resolution NanoLC-ESI-MS/MS

Jeanne,

Bernay,

Sourdaine

2023

J. Proteome Res.

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Spermatogenesis is a highly specialized process of cell proliferation and differentiation leading to the production of spermatozoa from spermatogonial stem cells. Due to its testicular anatomy, Scyliorhinus canicula is an interesting model to explore stage-based changes in proteins during spermatogenesis. The proteomes of four testicular zones corresponding to the germinative niche and to spermatocysts (cysts) with spermatogonia (zone A), cysts with spermatocytes (zone B), cysts with young spermatids (zone C), and cysts with late spermatids (zone D) have been analyzed by nanoLC-ESI-MS/MS. Gene ontology and KEGG annotations were also performed. A total of 3346 multiple protein groups were identified. Zone-specific protein analyses highlighted RNA-processing, chromosome-related processes, cilium organization, and cilium activity in zones A, D, C, and D, respectively. Analyses of proteins with zone-dependent abundance revealed processes related to cellular stress, ubiquitin-dependent degradation by the proteasome, post-transcriptional regulation, and regulation of cellular homeostasis. Our results also suggest that the roles of some proteins, such as ceruloplasmin, optineurin, the pregnancy zone protein, PA28β or the Culling-RING ligase 5 complex, as well as some uncharacterized proteins, during spermatogenesis could be further explored. Finally, the study of this shark species allows one to integrate these data in an evolutionary context of the regulation of spermatogenesis. Mass spectrometry data are freely accessible via iProX-integrated Proteome resources () for reuse purposes.

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Section: Resultsmentioning

confidence: 99%

Comparative Proteome Analysis of Four Stages of Spermatogenesis in the Small-Spotted Catshark (Scyliorhinus canicula), Using High-Resolution NanoLC-ESI-MS/MS

Jeanne,

Bernay,

Sourdaine

2023

J. Proteome Res.

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“…Furthermore, FNIP1, FNIP2, and Tsc1 alone and in combination enhance steroid hormone receptor ligand binding and activity. He proposed a model where the formation of different populations of Hsp90: cochaperone: client complexes provides differentially primed pools of client proteins ready to act in different cellular environments (Backe et al 2022 ).…”

Section: Hsp90 Interactions With Cochaperones Chaperones and Client P...mentioning

confidence: 99%

Tenth International Symposium on the Hsp90 chaperone machine

Edkins

Zweckstetter

Sawarkar

2023

Cell Stress and Chaperones

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Hsp90 is a molecular chaperone responsible for regulating proteostasis under physiological and pathological conditions. Its central role in a range of diseases and potential as a drug target has focused efforts to understand its mechanisms and biological functions and to identify modulators that may form the basis for therapies. The 10th international conference on the Hsp90 chaperone machine was held in Switzerland in October 2022. The meeting was organized by Didier Picard (Geneva, Switzerland) and Johannes Buchner (Garching, Germany) with an advisory committee of Olivier Genest, Mehdi Mollapour, Ritwick Sawarkar, and Patricija van Oosten-Hawle. This was a much anticipated first in-person meeting of the Hsp90 community since 2018 after the COVID-19 pandemic led to the postponement of the 2020 meeting. The conference remained true to the tradition of sharing novel data ahead of publication, providing unparalleled depth of insight for both experts and newcomers to the field.

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“…In the mammary gland, GR was found strongly localized in the nuclei of myoepithelial cells surrounding lobular and duct units and occasionally localized in the nuclei of stromal and endothelial cells (74). The GR nuclear localization indicates that the receptor is transcriptionally active, since the inactive GR resides mainly in the cytoplasm bound to heat shock proteins and immunophilins (14,75).…”

Section: Gr In Mammary Epithelial Cells: Its Dual Role In Cell Prolif...mentioning

confidence: 99%

“…Here we will focus on PR and GR, two receptors that shared several features ranging from several aspects of ligand-binding to the DNA sequences to which the receptor binds (Figure 1A ii). In the absence of ligands, these receptors are part of a protein complex associated with chaperones and co-chaperones, which increase the affinity of SHRs to their ligands in vivo (14). Early reconstitution experiments with GR (15) and with PR established that the central proteins in the activation pathway include Hsp40, Hsp70, Hsp90, HOP, and p23 (16,17).…”

Section: Introductionmentioning

confidence: 99%

Choosing the right partner in hormone-dependent gene regulation: Glucocorticoid and progesterone receptors crosstalk in breast cancer cells

et al. 2022

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Steroid hormone receptors (SHRs) belong to a large family of ligand-activated nuclear receptors that share certain characteristics and possess others that make them unique. It was thought for many years that the specificity of hormone response lay in the ligand. Although this may be true for pure agonists, the natural ligands as progesterone, corticosterone and cortisol present a broader effect by simultaneous activation of several SHRs. Moreover, SHRs share structural and functional characteristics that range from similarities between ligand-binding pockets to recognition of specific DNA sequences. These properties are clearly evident in progesterone (PR) and glucocorticoid receptors (GR); however, the biological responses triggered by each receptor in the presence of its ligand are different, and in some cases, even opposite. Thus, what confers the specificity of response to a given receptor is a long-standing topic of discussion that has not yet been unveiled. The levels of expression of each receptor, the differential interaction with coregulators, the chromatin accessibility as well as the DNA sequence of the target regions in the genome, are reliable sources of variability in hormone action that could explain the results obtained so far. Yet, to add further complexity to this scenario, it has been described that receptors can form heterocomplexes which can either compromise or potentiate the respective hormone-activated pathways with its possible impact on the pathological condition. In the present review, we summarized the state of the art of the functional cross-talk between PR and GR in breast cancer cells and we also discussed new paradigms of specificity in hormone action.

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A specialized Hsp90 co-chaperone network regulates steroid hormone receptor response to ligand

Cited by 23 publications

References 83 publications

Comparative Proteome Analysis of Four Stages of Spermatogenesis in the Small-Spotted Catshark (Scyliorhinus canicula), Using High-Resolution NanoLC-ESI-MS/MS

Comparative Proteome Analysis of Four Stages of Spermatogenesis in the Small-Spotted Catshark (Scyliorhinus canicula), Using High-Resolution NanoLC-ESI-MS/MS

Tenth International Symposium on the Hsp90 chaperone machine

Choosing the right partner in hormone-dependent gene regulation: Glucocorticoid and progesterone receptors crosstalk in breast cancer cells

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