A specific DNA-nanoprobe for tracking the activities of human apurinic/apyrimidinic endonuclease 1 in living cells

DNA repair is now understood to play a key role in a variety of disease states, most notably cancer. Tools for studying DNA have typically relied on traditional biochemical methods which are often laborious and indirect. Efforts to study the biology and therapeutic relevance of DNA repair pathways can be limited by such methods. Recently, specific fluorescent probes have been developed to aid in the study of DNA repair. Fluorescent probes offer the advantage of being able to directly assay for DNA repair activity in a simple, mix-and-measure format. This review will summarize the distinct classes of probe designs and their potential utility in varied research and preclinical settings.

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“…In one recent example, an on-bead probe of APE 1 was used in cells to explore APE 1 repair activity in tumors. 41 …”

Section: Molecular Beacon Probesmentioning

confidence: 99%

Fluorescent Probes of DNA Repair

Wilson

Kool

2017

ACS Chem. Biol.

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“…Nanoparticles (NPs) had been shown to effectively increase pDNA delivery to target cells or tissues . In addition to highly efficient gene delivery, another advantage of using NPs as a carrier is that NPs can protect pDNA against DNase digestion in blood circulation following intravenous injection . To date no combination of miRNA plasmids and nanomaterials has been proposed to treat ADPKD.…”

Section: Introductionmentioning

confidence: 99%

Safe Nanocomposite‐Mediated Efficient Delivery of MicroRNA Plasmids for Autosomal Dominant Polycystic Kidney Disease (ADPKD) Therapy

Tsai

Teng

Jiang

et al. 2019

Adv Healthcare Materials

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There is currently no cure for gene mutation‐caused autosomal dominant polycystic kidney disease (ADPKD). Over half of patients with ADPKD eventually develop kidney failure, requiring dialysis or kidney transplantation. Current treatment modalities for ADPKD focus on reducing morbidity and mortality from renal and extrarenal complications of the disease. MicroRNA has been shown to be useful in treating ADPKD. This study combines anti‐miRNA plasmids and iron oxide/alginate nanoparticles for conjugation with antikidney antibodies. These nanocomposites can specifically target renal tubular cells, providing a potential treatment for ADPKD. Magnetic resonance imaging and in vivo imaging system results show effective targeting of renal cells. Anti‐miRNA plasmids released from the nanocomposites inhibit cell proliferation and cyst formation in the PKD cellular and animal models. The results suggest the novel combination of the anti‐miRNA plasmids and nanomaterials provides potential clinical implications for ADPKD treatment.

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“…However, these methods tend to be tedious and time-consuming process, and may require specialist instrumentation and/or expertise. Recently, optical strategies for real-time and in-field sensing of biomarkers have been explored, including methods based on green fluorescent protein [14], DNA sequence [15][16][17][18], nanoparticles [19][20][21], quantum dots [22][23][24], organic dyes [25,26] and metal complexes [27]. Fluorescent organic dyes, in particular, have received intense interest for biomarker detection.…”

Section: Introductionmentioning

confidence: 99%

Group 8–9 Metal-Based Luminescent Chemosensors for Protein Biomarker Detection

et al. 2018

J. Anal. Test.

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The development of transition metal complex-based luminescent chemosensors has recently aroused increasing interest for protein biomarker labelling and detection, especially for the real-time diagnosis and treatment of disease. This is owing to their unique photophysical properties, particularly their long-lived and environmentally sensitive emission, which can be easily controlled via the structural modification of ligands. In this overview, we highlight recent examples of protein biomarker detection using group 8-9 metal-based luminescent chemosensors, including the frequently employed ruthenium(II) and iridium(III) complexes. Various mechanisms and sensing modes are described and compared, and the outlook and future directions of this field are discussed as well.

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A specific DNA-nanoprobe for tracking the activities of human apurinic/apyrimidinic endonuclease 1 in living cells

Cited by 56 publications

References 27 publications

Fluorescent Probes of DNA Repair

Fluorescent Probes of DNA Repair

Safe Nanocomposite‐Mediated Efficient Delivery of MicroRNA Plasmids for Autosomal Dominant Polycystic Kidney Disease (ADPKD) Therapy

Group 8–9 Metal-Based Luminescent Chemosensors for Protein Biomarker Detection

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