The poultry processing industry struggles to keep up with new developments in meat consumption and livestock breeding. Designing poultry processing systems is becoming increasingly more complex, and an increasing number of iterations of (re)design are required to optimize the product flow in these systems. To address this issue, this study presents a discrete-event simulation-based method for design space exploration of production systems. This method is mostly automated, greatly reducing the time and effort required in the design process. The steps that are automated are iterating on the design, model construction, performing simulation experiments, and interpreting the simulation results. An industrial case study in which a poultry processing system is redesigned is used to validate the effectiveness of the proposed method. A detailed description of this case study is given to showcase the different ways in which this design space exploration method can be used.