A spectrophotometric method for determination of glucose in blood serum. A freshman laboratory experiment for medically and biologically oriented students

Abstract

A freshman laboratory experiment for medically and biologically oriented students Colorimetric or spectrophotometric methods based upon the reaction of glucose with primary aromatic amines in glacial acetic acid have been utilized clinically with different degrees of success.1,2 Perhaps the most successful is the selective reaction of glucose with o-toluidine, a primary ar-

Effect of Developed Low Cost Minimal Medium on Lipid and Exopolysaccharide Production by Lipomyces starkeyi Under Repeated Fed-batch and Continuous Cultivation

“…Following 2 h incubation in the freezer, the solution was centrifuged at 12,000 g for 10 min (SorvallStratos Benchtop Centrifuge -Thermo Fisher Scientific, U.S.A.), and the pellet (if any) was separated from the supernatant. The supernatant was analyzed for glucose concentration using the Daines colorimetric method 20 , and a calibration curve relating absorbance and glucose concentration was used to report glucose concentration (g glucose L -1 ). The pellet was suspended in deionized water and analyzed for polysaccharides using the phenol-sulfuric acid method 21 .…”