A spectrophotometric method for the determination of zinc, copper, and cobalt ions in metalloproteins using Zincon

Säbel, Crystal E.; Neureuther, Joseph M.; Siemann, Stefan

doi:10.1016/j.ab.2009.10.037

Cited by 177 publications

(125 citation statements)

References 52 publications

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“…• The absorbance of the solutions was measured on UV-VIS V-630 spectrophotometer (JASCO, Japan) equipped with quartz cuvettes at 230.0 and 241.4 nm for EDDS and EDTA, respectively, due to the maximum absorbance of the investigated complexes (Welcher 1958;Säbel et al 2010). …”

Section: Pb Content In the Incubation Mediummentioning

confidence: 99%

The effect of EDTA and EDDS on lead uptake and localization in hydroponically grown Pisum sativum L.

et al. 2013

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Pisum sativum plants were treated for 3 days with an aqueous solution of 100 lM Pb(NO 3 ) 2 or with a mixture of lead nitrate and ethylenediaminetetraacetic acid (EDTA) or [S,S]-ethylenediaminedisuccinic acid (EDDS) at equimolar concentrations. Lead decline from the incubation media and its accumulation and localization at the morphological and ultrastructural levels as well as plant growth parameters (root growth, root and shoot dry weight) were estimated after 1 and 3 days of treatment. The tested chelators, especially EDTA, significantly diminished Pb uptake by plants as compared to the lead nitrate-treated material. Simultaneously, EDTA significantly enhanced Pb translocation from roots to shoots. In the presence of both chelates, plant growth parameters remained considerably higher than in the case of uncomplexed Pb. Considerable differences between the tested chelators were visible in Pb localization both at the morphological and ultrastructural level. In Pb?EDTA-treated roots, lead was mainly located in the apical parts, while in Pb?EDDS-exposed material Pb was evenly distributed along the whole root length.Transmission electron microscopy and EDS analysis revealed that in meristematic cells of the roots incubated in Pb?EDTA, large electron-dense lead deposits were located in vacuoles and small granules were rarely noticed in cell walls or cytoplasm, while after Pb?EDDS treatment metal deposits were restricted to the border between plasmalemma and cell wall. Such results imply different ways of transport of those complexed Pb forms.

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Section: Pb Content In the Incubation Mediummentioning

confidence: 99%

The effect of EDTA and EDDS on lead uptake and localization in hydroponically grown Pisum sativum L.

et al. 2013

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“…This formazan dye has been shown to serve as an excellent chromophore for the quantification of both elements in aqueous solutions of particular relevance in biological systems such as metalloproteins. 19 Although zincon could be used as a spectrophotometric reagent for zinc and copper, at a pH above 8 it reacts only with zinc.…”

Section: Introductionmentioning

confidence: 99%

On-line Determination of Zinc in Water and Biological Samples after Its Preconcentration onto Zincon Anchored Polyurethane Foam

2015

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A fast and sensitive on-line procedure for the determination of zinc in water and biological samples was developed. Zinc was preconcentrated in a mini-column packed with polyurethane foam (PUF) chemically modified with zincon via -N=N-bonding. The optimal conditions for preconcentration were pH 8.5 and sample flow rate of 4.0 mL min -1 . Quantitative desorption of Zn(II) was obtained by 0.1 mol L -1 hydrochloric acid and subsequent spectrophotmetric determination using 4-(2-pyridylazo)-resorcinol at 498 nm. The obtained detection limit was found to be 3.0 ng mL -1 , precision (RSD) was 4.8 and 6.7% at 20 and 110 ng mL -1 , respectively, for 60 s preconcentration time and enrichment factor was 31. The linearity range was from 10 to 120 ng mL -1 and maximum sample throughput was 20 h -1 . Finally, the method was successfully applied to the determination of zinc in tap water, Nile River water and human urine samples with RSD in the range of 1.1 -8.3%.

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“…Over the years, UV-Vis spectrophotometry has been widely used for the determination of metals [2][3][4][5][6], because it is an available and inexpensive technique which can be found in most laboratories. However, due to low concentration of heavy metals, insufficient sensitivity of the instrument, and matrix interferences, there are some difficulties for determination of traces of such analytes using this technique.…”

Section: Introductionmentioning

confidence: 99%

Determination of Zinc, Copper, and Mercury in Water Samples by Using Novel Micro Cloud Point Extraction and UV-Vis Spectrophotometry

Ghasemi¹,

Kaykhaii²

2016

Eurasian J Anal Chem

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A novel micro cloud point extraction (MCPE) was developed as a fast, simple, and economical preconcentration method for spectrophotometric determination of Zn, Cu, and Hg in water samples. In contrary to traditional cloud point extraction, this method does not need heating. To achieve the cloud point in room temperature, the MCPE procedure was carried out in brine. Triton X-114 was employed as a non-ionic surfactant and the analytes were chelated by 4-(2-pyridylazo) resorcinol prior to extraction. The important factors influencing the extraction efficiency were investigated and optimized. Under the optimized condition, calibration curve was found to be linear in the concentration range of 0.15-0.60, 0.02-0.10, and 0.30-0.80 mg.L -1 for Zn, Cu, and Hg respectively with a limit of detection of 51.7, 9.8, and 13.1 μg.L -1 .

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A spectrophotometric method for the determination of zinc, copper, and cobalt ions in metalloproteins using Zincon

Cited by 177 publications

References 52 publications

The effect of EDTA and EDDS on lead uptake and localization in hydroponically grown Pisum sativum L.

The effect of EDTA and EDDS on lead uptake and localization in hydroponically grown Pisum sativum L.

On-line Determination of Zinc in Water and Biological Samples after Its Preconcentration onto Zincon Anchored Polyurethane Foam

Determination of Zinc, Copper, and Mercury in Water Samples by Using Novel Micro Cloud Point Extraction and UV-Vis Spectrophotometry

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