A Speculative Comparison of Allotypic Suppression and Allotypic Blast Transformation: Control of Expression of T and B Cell Immunoglobulin Allotypic Markers by a B Cell Product

Alternative Hypotheses of Lymphocyte Surface Immunoglobulin Expression, B Lymphocyte Activation and B Lymphocyte Differentiation

“…A similar dose-time blocking effect occurs with anti-Ig stimulation and Ig blocking , Sell 1974. Small lymphocytes that enlarge during the early stages of transformation actually revert back to small lymphocytes after blocking without division (Sell 1974). The results are consistent with the interpretation that most if not all ofthe stimulated cells pass through the stages prior to S at about the same rate and that little or no recruitment of new cells occurs after the initial stimulation time.…”

“…The dose-time kineticsof blocking of Con A stimulated transformation is shown in Figure 2 (Sell & Sheppard 1974. A similar dose-time blocking effect occurs with anti-Ig stimulation and Ig blocking , Sell 1974. Small lymphocytes that enlarge during the early stages of transformation actually revert back to small lymphocytes after blocking without division (Sell 1974).…”

“…Antiallotypic serum produces suppression of the expression of the reactive allotypic markers (allotype suppression). This appears to be induced on the cellular level by endocytosis of the reactive allotype and enhanced expression of the unreactive allelic marker (Sell 1975). Such restriction of expression occurs normally during differentiation of plasma cells.…”

“…The bottom line indicates that stimulation with Con A for 12 h. followed by n-M AM block and restimulation by PHA, results in a much greater effect than the sum of the separate responses in Con A-a-MAM blocked cultures, after delayed PHA addition, or with continuous stimulation by PHA. These results are interpreted to mean that many cells may be "preactivated" by Con A to respond to subsequent stimulation by PHA (for details see Sell et al 1975).Exact blast percentage is difficult to determine due to agglutination of cells by PHA.…”

“…The alternative hypotheses are supported by data obtained by analysis ofthe rabbit system as well as selected studies in mice and germ free guinea pigs. The subject of allotypic suppression, a truly remarkable effect of anti-Ig on B cell expression, was recently reviewed in this series (Sell 1975) and will not be discussed further in this article. BACKGROUND Lymphocyte mitogenicity, the ability of certain exogenous agents to stimulate small lymphocytes to transfer into large "blast" cells and divide was discovered in 1960by Peter Nowell and has been used extensively in many basic and clinical studies.…”