A split-GAL4 driver line resource for<i>Drosophila</i>neuron types

Meissner, Geoffrey W; Vannan, Allison; Jeter, Jennifer; Close, Kari; DePasquale, Gina M; Dorman, Zachary; Forster, Kaitlyn; Beringer, Jaye Anne; Gibney, Theresa V; Hausenfluck, Joanna H; He, Yisheng; Henderson, Kristin; Johnson, Lauren; Johnston, Rebecca M; Ihrke, Gudrun; Iyer, Nirmala; Lazarus, Rachel; Lee, Kelley; Li, Hsing-Hsi; Liaw, Hua-Peng; Melton, Brian; Miller, Scott; Motaher, Reeham; Novak, Alexandra; Ogundeyi, Omotara; Petruncio, Alyson; Price, Jacquelyn; Protopapas, Sophia; Tae, Susana; Taylor, Jennifer; Vorimo, Rebecca; Yarbrough, Brianna; Zeng, Kevin Xiankun; Zugates, Christopher T; Dionne, Heather; Angstadt, Claire; Ashley, Kelly; Cavallaro, Amanda; Dang, Tam; Gonzalez, Guillermo A; Hibbard, Karen L; Huang, Cuizhen; Kao, Jui-Chun; Laverty, Todd; Mercer, Monti; Perez, Brenda; Pitts, Scarlett; Ruiz, Danielle; Vallanadu, Viruthika; Zheng, Grace Zhiyu; Goina, Cristian; Otsuna, Hideo; Rokicki, Konrad; Svirskas, Robert R; Cheong, Han SJ; Dolan, Michael-John; Ehrhardt, Erica; Feng, Kai; Galfi, Basel El; Goldammer, Jens; Huston, Stephen J; Hu, Nan; Ito, Masayoshi; McKellar, Claire; Minegishi, Ryo; Namiki, Shigehiro; Nern, Aljoscha; Schretter, Catherine E; Sterne, Gabriella R; Venkatasubramanian, Lalanti; Wang, Kaiyu; Wolff, Tanya; Wu, Ming; George, Reed; Malkesman, Oz; Aso, Yoshinori; Card, Gwyneth M; Dickson, Barry J; Korff, Wyatt; Ito, Kei; Truman, James W; Zlatic, Marta; Rubin, Gerald M; ,

doi:10.1101/2024.01.09.574419

Cited by 9 publications

(3 citation statements)

References 100 publications

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“…We aligned the expression pattern in the VNC using the Computational Morphometry Toolkit (CMTK; Neuroimaging Informatics Tools and Resources Clearinghouse) to a female VNC template 64 in Fiji 65 . Confocal stacks of the claw line, one of the hook flexion lines (driver line 1), and the web line were downloaded from the GAL4 and split-GAL4 collections on FlyLight 66,67 .…”

Section: Star Methodsmentioning

confidence: 99%

Presynaptic inhibition selectively suppresses leg proprioception in behavingDrosophila

Dallmann,

Luo,

Agrawal

et al. 2023

Preprint

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The sense of proprioception is mediated by internal mechanosensory neurons that detect joint position and movement. To support a diverse range of functions, from stabilizing posture to coordinating movements, proprioceptive feedback to limb motor control circuits must be tuned in a context-dependent manner. How proprioceptive feedback signals are tuned to match behavioral demands remains poorly understood. Using calcium imaging in behavingDrosophila, we find that the axons of position-encoding leg proprioceptors are active across behaviors, whereas the axons of movement-encoding leg proprioceptors are suppressed during walking and grooming. Using connectomics, we identify a specific class of interneurons that provide GABAergic presynaptic inhibition to the axons of movement-encoding proprioceptors. These interneurons are active during self-generated but not passive leg movements and receive input from descending neurons, suggesting they are driven by predictions of leg movement originating in the brain. Predictively suppressing expected proprioceptive feedback provides a mechanism to attenuate reflexes that would otherwise interfere with voluntary movement.

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Section: Star Methodsmentioning

confidence: 99%

Presynaptic inhibition selectively suppresses leg proprioception in behavingDrosophila

Dallmann,

Luo,

Agrawal

et al. 2023

Preprint

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“…Most split-Gal4 screening, sample preparation and imaging were performed by the FlyLight Project Team, using established methods 20,32,62,66 and publicly available protocols (https://www.janelia.org/project-team/flylight/protocols). The FlyLight split-GAL4 project has been summarized in a recent publication 67 .…”

Section: Methodsmentioning

confidence: 99%

Connectome-driven neural inventory of a complete visual system

Nern,

Loesche,

Takemura

et al. 2024

Preprint

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Vision provides animals with detailed information about their surroundings, conveying diverse features such as color, form, and movement across the visual scene. Computing these parallel spatial features requires a large and diverse network of neurons, such that in animals as distant as flies and humans, visual regions comprise half the brain's volume. These visual brain regions often reveal remarkable structure-function relationships, with neurons organized along spatial maps with shapes that directly relate to their roles in visual processing. To unravel the stunning diversity of a complex visual system, a careful mapping of the neural architecture matched to tools for targeted exploration of that circuitry is essential. Here, we report a new connectome of the right optic lobe from a male Drosophila central nervous system FIB-SEM volume and a comprehensive inventory of the fly's visual neurons. We developed a computational framework to quantify the anatomy of visual neurons, establishing a basis for interpreting how their shapes relate to spatial vision. By integrating this analysis with connectivity information, neurotransmitter identity, and expert curation, we classified the ~53,000 neurons into 727 types, about half of which are systematically described and named for the first time. Finally, we share an extensive collection of split-GAL4 lines matched to our neuron type catalog. Together, this comprehensive set of tools and data unlock new possibilities for systematic investigations of vision in Drosophila, a foundation for a deeper understanding of sensory processing.

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“…DNs from LM images were identified by overlaying the EM reconstructed DNs with images of Gal4 lines, mainly from the Namiki collection ( (Namiki et al, 2018) and in preparation Namiki et al 2024), Janelia's Gal4 and Split-Gal4 collections (Jenett et al, 2012;Meissner et al, 2024;Tirian & Dickson, 2017), or via the neuronbridge tool (Clements et al, 2022;Meissner et al, 2023) for MANC DNs. To compare the reconstructions and LM images in the same space, the latter were segmented and transformed into MANC space as described in (H. S. J.…”

Section: Light Microscopy Identificationmentioning

confidence: 99%

Comparative connectomics of the descending and ascending neurons of theDrosophilanervous system: stereotypy and sexual dimorphism

Stürner,

Brooks,

Capdevila

et al. 2024

Preprint

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In most complex nervous systems there is a clear anatomical separation between the nerve cord, which contains most of the final motor outputs necessary for behaviour, and the brain. In insects, the neck connective is both a physical and information bottleneck connecting the brain and the ventral nerve cord (VNC, spinal cord analogue) and comprises diverse populations of descending (DN), ascending (AN) and sensory ascending neurons, which are crucial for sensorimotor signalling and control.Integrating three separate EM datasets, we now provide a complete connectomic description of the ascending and descending neurons of the female nervous system ofDrosophilaand compare them with neurons of the male nerve cord. Proofread neuronal reconstructions have been matched across hemispheres, datasets and sexes. Crucially, we have also matched 51% of DN cell types to light level data defining specific driver lines as well as classifying all ascending populations.We use these results to reveal the general architecture, tracts, neuropil innervation and connectivity of neck connective neurons. We observe connected chains of descending and ascending neurons spanning the neck, which may subserve motor sequences. We provide a complete description of sexually dimorphic DN and AN populations, with detailed analysis of circuits implicated in sex-related behaviours, including female ovipositor extrusion (DNp13), male courtship (DNa12/aSP22) and song production (AN hemilineage 08B). Our work represents the first EM-level circuit analyses spanning the entire central nervous system of an adult animal.

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A split-GAL4 driver line resource forDrosophilaneuron types

Cited by 9 publications

References 100 publications

Presynaptic inhibition selectively suppresses leg proprioception in behavingDrosophila

Presynaptic inhibition selectively suppresses leg proprioception in behavingDrosophila

Connectome-driven neural inventory of a complete visual system

Comparative connectomics of the descending and ascending neurons of theDrosophilanervous system: stereotypy and sexual dimorphism

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