A Stable Bioluminescent Construct of
            <i>Escherichia coli</i>
            O157:H7 for Hazard Assessments of Long-Term Survival in the Environment

Ritchie, Jennifer M.; Campbell, G.R.; Shepherd, Jill; Beaton, Yvonne; Jones, Davey L.; Killham, Ken; Artz, Rebekka

doi:10.1128/aem.69.6.3359-3367.2003

Cited by 67 publications

(74 citation statements)

References 52 publications

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“…For instance, two E. coli strains, C278 and C279 (different by enterobacterial repetitive intergenic consensus sequence (ERIC)-PCR-based genomic fingerprinting), survived differently in soil amended with swine manure (Topp et al, 2003). Moreover, basal production of toxin (Stx) by haemolytic uraemic syndrome-associated E. coli was higher than that by bovineassociated E. coli strains (Ritchie et al, 2003). In addition, a reconsideration of the work of Kudva et al (1998) showed that at 23 1C a toxin-negative strain of E. coli O157:H7 grew and survived better than its toxin-positive counterpart, whereas no such differences were found at lower temperatures.…”

Section: Presence and Diversity Of An Indigenous Microfloramentioning

confidence: 99%

“…E. coli O157:H7 is dangerous because of its resistance to low pH (B2.5), which allows passage through the stomach, its low infective dose (as few as 10 cells) and its high pathogenicity (Tilden et al, 1996). Following tissue invasion, it may even cause death (Ritchie et al, 2003). Moreover, the stx genes were found to be transferable to non-pathogenic E. coli strains, allowing these to enhance their virulence (Herold et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Survival of Escherichia coli in the environment: fundamental and public health aspects

et al. 2010

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In this review, our current understanding of the species Escherichia coli and its persistence in the open environment is examined. E. coli consists of six different subgroups, which are separable by genomic analyses. Strains within each subgroup occupy various ecological niches, and can be broadly characterized by either commensalistic or different pathogenic behaviour. In relevant cases, genomic islands can be pinpointed that underpin the behaviour. Thus, genomic islands of, on the one hand, broad environmental significance, and, on the other hand, virulence, are highlighted in the context of E. coli survival in its niches. A focus is further placed on experimental studies on the survival of the different types of E. coli in soil, manure and water. Overall, the data suggest that E. coli can persist, for varying periods of time, in such terrestrial and aquatic habitats. In particular, the considerable persistence of the pathogenic E. coli O157:H7 is of importance, as its acid tolerance may be expected to confer a fitness asset in the more acidic environments. In this context, the extent to which E. coli interacts with its human/animal host and the organism's survivability in natural environments are compared. In addition, the effect of the diversity and community structure of the indigenous microbiota on the fate of invading E. coli populations in the open environment is discussed. Such a relationship is of importance to our knowledge of both public and environmental health.

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Section: Presence and Diversity Of An Indigenous Microfloramentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Survival of Escherichia coli in the environment: fundamental and public health aspects

et al. 2010

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“…In both microcosm experiments, after allowing 30 d for the establishment and stabilization of the microbial community at a comparable level across all of the treatments, an invading bacterial pathogen [i.e., the genetically marked, nonpathogenic E. coli O157:H7 derivative strain Tn5 luxCDAEB (20)] was introduced into the soil microcosms at approximately 10 8 cfu g −1 dry soil. This established a soil moisture content of 75% of WHC.…”

Section: Methodsmentioning

confidence: 99%

“…The fate of the enterohemorrhagic Escherichia coli (EHEC) O157:H7 in soil is of major concern (18)(19)(20). In this context, the microbial communities that established after soil fumigation were shown to determine the fate of the invading species, whereby reduction in microbial diversity due to progressively enhanced fumigation depths resulted in higher pathogen persistence in soil (21).…”

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Microbial diversity determines the invasion of soil by a bacterial pathogen

Elsas

Chiurazzi

Mallon

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

826

566

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Natural ecosystems show variable resistance to invasion by alien species, and this resistance can relate to the species diversity in the system. In soil, microorganisms are key components that determine life support functions, but the functional redundancy in the microbiota of most soils has long been thought to overwhelm microbial diversity–function relationships. We here show an inverse relationship between soil microbial diversity and survival of the invading species Escherichia coli O157:H7, assessed by using the marked derivative strain T. The invader's fate in soil was determined in the presence of ( i ) differentially constructed culturable bacterial communities, and ( ii ) microbial communities established using a dilution-to-extinction approach. Both approaches revealed a negative correlation between the diversity of the soil microbiota and survival of the invader. The relationship could be explained by a decrease in the competitive ability of the invader in species-rich vs. species-poor bacterial communities, reflected in the amount of resources used and the rate of their consumption. Soil microbial diversity is a key factor that controls the extent to which bacterial invaders can establish.

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Effect of granular activated carbon concentration on the content of organic matter and salt, influencing E. coli activity and survival in fluidized bed disinfection reactor

Racyte

Langenhoff

Ribeiro³

et al. 2014

Biotech & Bioengineering

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Granular activated carbon (GAC) is used in water treatment systems, typically to remove pollutants such as natural organic matter, volatile organic compounds, chlorine, taste, and odor. GAC is also used as a key component of a new technology that combines a fluidized bed reactor with radio frequency electric fields for disinfection. So far, the effects of GAC on bacteria in these fluidized bed reactors are unclear. This paper describes a systematic study of the physico-chemical changes in five microbial media compositions caused by different concentrations (23-350 g/L) of GAC, and the effects of these physico-chemical changes on the metabolic activity and survival of a model microorganism (Escherichia coli YMc10) in a fluidized bed reactor. The chemical adsorption taking place in suspensions with specific GAC changed nutritional, osmotic, and pH conditions in the investigated microbial media (LB, diluted LB, PBS, diluted PBS, and tap water), leading to a decay of the metabolic activity and survival of E. coli. Especially media that are poor in organic and mineral compounds (e.g., PBS) with suspended GAC showed a concentration decay of 3.5 Log CFU/mL E. coli after 6 h. Organic compounds depletion and severe pH variation were enhanced in the presence of higher GAC concentrations.

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A Stable Bioluminescent Construct of Escherichia coli O157:H7 for Hazard Assessments of Long-Term Survival in the Environment

Cited by 67 publications

References 52 publications

Survival of Escherichia coli in the environment: fundamental and public health aspects

Survival of Escherichia coli in the environment: fundamental and public health aspects

Microbial diversity determines the invasion of soil by a bacterial pathogen

Effect of granular activated carbon concentration on the content of organic matter and salt, influencing E. coli activity and survival in fluidized bed disinfection reactor

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